Upregulated GAPLINC predicts a poor prognosis in bladder cancer patients and promotes tumor proliferation and invasion

Zheng, Zijie; Zhu, Dingjun; Zhao, Fengli; Han, Jing; Chen, Haicheng; Cai, Yuhong; Xie, Wenlian

doi:10.3892/ol.2018.8158

Cited by 4 publications

(6 citation statements)

References 31 publications

(28 reference statements)

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“…In gastric cancer, GAPLINC was shown to promote cell proliferation and migration by regulating CD44 expression [18] . Pro-oncogenic effects of GAPLINC in other cancers, including CRC [49] , NSCLC [50] and bladder cancer [51] have also been reported. We identi ed GAPLINC as our hub lncRNA, and it was determined that GAPLINC is a risk factor for CCA and a predictor for poor prognosis.…”

Section: Discussionmentioning

confidence: 94%

Identification and Validation of lncRNA-Related Prognostic Signatures in Cholangiocarcinoma

Zhang

Huang

et al. 2021

Preprint

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Background and Aims: Cholangiocarcinoma (CCA), the second most common hepatobiliary cancer, is associated with poor prognosis. Therefore, there is a need to elucidate on the pathogenic mechanisms of CCA. In this study, we aimed at identifying lncRNA-related prognostic signatures for CCA through bioinformatics analysis and further validated their functions in CCA tumorigenesis and progression.Methods: The RNA-seq data of CCA were downloaded from public databases. Differentially expressed lncRNAs (DElncRNAs) were screened using R packages. Then, candidate OS- and DFS-related DElncRNAs were selected through Kaplan–Meier survival analysis. Furthermore, LASSO regression analyses were performed to establish two prognostic signatures, termed the OS and DFS signatures, respectively. Multivariate COX models and nomograms for overall survival (OS) and disease-free survival (DFS) were established based on OS/DFS signature and clinical data. Hub lncRNAs were identified and enrichment analyses performed to explore their potential functions. Finally, in vitro and in vivo models were used to validate the effects of the hub lncRNAs in CCA tumorigenesis and progression.Results: A total of 925 DElncRNAs were selected, from which six candidate OS-related lncRNAs and 15 candidate DFS-related lncRNAs were identified. The OS and DFS signatures were then established using four lncRNAs, respectively. We found that the OS signature and vascular invasion were significant independent risk factors for OS outcomes of CCA, while the DFS signature, vascular invasion and CA19-9 were significant independent risk factors for DFS outcomes of CCA. MiR4435-2HG and GAPLINC were selected as hub lncRNAs because they were included in both OS and DFS signatures. GO and KEGG enrichment analyses revealed that the two hub lncRNAs were involved in CCA tumorigenesis and progression. Finally, we constructed in vitro and in vivo models and revealed that the lncRNAs, MiR4435-2HG and GAPLINC can prompt CCA proliferation and migration in vitro and in vivo.Conclusions: The established OS and DFS signatures, which were based on DElncRNAs, are independent risk factors for OS and DFS of CCA patients, respectively. MIR4435-2HG and GAPLINC were identified as hub lncRNAs. In vitro and in vivo models revealed that MiR4435-2HG and GAPLINC can prompt CCA progression, which might be novel prognostic biomarkers and therapeutic targets for CCA.

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Section: Discussionmentioning

confidence: 94%

Identification and Validation of lncRNA-Related Prognostic Signatures in Cholangiocarcinoma

Zhang

Huang

et al. 2021

Preprint

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“…Moreover, several evidences showed that GAPLINC was also usually highly expressed in colorectal cancer tissues compared with adjacent normal tissues [ 13–15 ]. Besides, Zheng et al [ 16 ] observed GAPLINC express was elevated in bladder cancer tissue compared with normal tissues through TCGA database, and the high levels of GAPLINC expression were confirmed in 80 pairs of bladder cancer tissues and adjacent normal tissues by RT-qPCR. However, the expression status of GAPLINC in osteosarcoma is still unknown.…”

Section: Discussionmentioning

confidence: 99%

“…In colorectal cancer patients, Yang et al [ 14 ] found that high levels of GAPLINC expression were associated with large tumor size, advanced tumor stage (T classification), and advanced lymph node stage (N classification). Moreover, Zheng et al [ 16 ] indicated that levels of GAPLINC expression were correlared with tumor stage in bladder cancer cases.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the prognostic value of GAPLINC expression has been suggested in gastric cancer [ 12 , 20 ], colorectal cancer [ 14 ], and bladder cancer [ 16 ]. Zheng et al [ 16 ] demonstrated that bladder cancer patients in the high GAPLINC expression group had significantly shorter overall survival time than patients in the low GAPLINC expression group. Moreover, Hu et al [ 12 ] and Liu et al [ 20 ] congruously indicated that high-expression of GAPLINC was correlated with unfavorable prognosis in gastric cancer cases.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, GAPLINC regulated tumor cell proliferation and migration through competing with CD44 for miR-211-3p [ 12 ]. Subsequently, the clinical significance and biological function of GAPLINC was also explored in colorectal [ 13–15 ] and bladder cancers [ 16 ]. However, the role of GAPLINC in osteosarcoma is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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GAPLINC is a predictor of poor prognosis and regulates cell migration and invasion in osteosarcoma

2018

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Gastric adenocarcinoma predictive long intergenic non-coding (GAPLINC) is a novel long non-coding RNA (lncRNA) and has been found to function as an oncogenic lncRNA in gastric cancer, colorectal cancer, and bladder cancer. The expression status and biological function of GAPLINC in osteosarcoma are still unknown. Thus, we analyzed the association between GAPLINC expression and clinicopathological characteristics in osteosarcoma clinical samples, and conducted loss-of-function study in osteosarcoma cell lines. In our results, GAPLINC expression is elevated in osteosarcoma tissues and cell lines, and correlated with advanced Enneking stage, present distant metastasis, and poor histological grade. Survival analyses indicated that GAPLINC expression was negatively associated with overall survival, and GAPLINC high-expression was an independent risk factor in osteosarcoma patients. The in vitro studies showed knockdown of GAPLINC depressed osteosarcoma cell migration and invasion via inhibiting CD44 expression, but no effect on cell proliferation. In conclusion, GAPLINC may serve as a potential biomarker for predicting prognosis and developing therapy for osteosarcoma.

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Identification of a four immune‐related genes signature based on an immunogenomic landscape analysis of clear cell renal cell carcinoma

Gào

Yang

Chen

2020

Journal Cellular Physiology

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Renal clear cell carcinoma (ccRCC) is the most common type of renal cell carcinoma, which has strong immunogenicity. A comprehensive study of the role of immune‐related genes (IRGs) in ccRCC is of great significance in finding ccRCC treatment targets and improving patient prognosis. In this study, we comprehensively analyzed the expression of IRGs in ccRCC based on The Cancer Genome Atlas datasets. The mechanism of differentially expressed IRGs in ccRCC was analyzed by bioinformatics. In addition, Cox regression analysis was used to screen prognostic related IRGs from differentially expressed IRGs. We also identified a four IRGs signature consisting of four IRGs (CXCL2, SEMA3G, PDGFD, and UCN) through lasso regression and multivariate Cox regression analysis. Further analysis results showed that the four IRGs signature could effectively predict the prognosis of patients with ccRCC, and its predictive power is independent of other clinical factors. In addition, the correlation analysis of immune cell infiltration showed that this four IRGs signature could effectively reflect the level of immune cell infiltration of ccRCC. We also found that the expression of immune checkpoint genes CTLA‐4, LAG3, and PD‐1 in the high‐risk group was higher than that in the low‐risk group. Our research revealed the role of IRGs in ccRCC, and developed a four IRGs signature that could be used to evaluate the prognosis of patients with ccRCC, which will help to develop personalized treatment strategies for patients with ccRCC and improve their prognosis. In addition, these four IRGs may be effective therapeutic targets for ccRCC.

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Upregulated GAPLINC predicts a poor prognosis in bladder cancer patients and promotes tumor proliferation and invasion

Cited by 4 publications

References 31 publications

Identification and Validation of lncRNA-Related Prognostic Signatures in Cholangiocarcinoma

Identification and Validation of lncRNA-Related Prognostic Signatures in Cholangiocarcinoma

GAPLINC is a predictor of poor prognosis and regulates cell migration and invasion in osteosarcoma

Identification of a four immune‐related genes signature based on an immunogenomic landscape analysis of clear cell renal cell carcinoma

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