Upregulated Expression of TUBA1C Predicts Poor Prognosis and Promotes Oncogenesis in Pancreatic Ductal Adenocarcinoma via Regulating the Cell Cycle

Albahde, Mugahed Abdullah Hasan; Zhang, Haining; Zhang, Qiuqiang; Li, Guoqi; Wang, Weilin

doi:10.3389/fonc.2020.00049

Cited by 29 publications

(36 citation statements)

References 35 publications

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“…The researches exposed that microtubules combined with many microtubule-related proteins such as g α-, β-, and γ-tubulin aggregate to achieve various cellular functions in the cell cycle (mitosis and meiosis) [ 17 , 18 ]. Moreover, TUBA1C, a subtype of α-tubulin, which is composed of microtubule structure, was reported to be overexpressed and promotes oncogenesis in pancreatic ductal adenocarcinoma via Regulating the cell cycle [ 19 ]. In this study, we also found that cells transfected with SARS-COV-2 N protein usually had a higher TUBA1C expression.…”

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of the SARS-COV-2 nucleocapsid protein

Gao

Liu

et al. 2021

BMC Microbiol

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Background A severe form of pneumonia, named coronavirus disease 2019 (COVID-19) by the World Health Organization is widespread on the whole world. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was proved to be the main agent of COVID-19. In the present study, we conducted an in depth analysis of the SARS-COV-2 nucleocapsid to identify potential targets that may allow identification of therapeutic targets. Methods The SARS-COV-2 N protein subcellular localization and physicochemical property was analyzed by PSORT II Prediction and ProtParam tool. Then SOPMA tool and swiss-model was applied to analyze the structure of N protein. Next, the biological function was explored by mass spectrometry analysis and flow cytometry. At last, its potential phosphorylation sites were analyzed by NetPhos3.1 Server and PROVEAN PROTEIN. Results SARS-COV-2 N protein composed of 419 aa, is a 45.6 kDa positively charged unstable hydrophobic protein. It has 91 and 49% similarity to SARS-CoV and MERS-CoV and is predicted to be predominantly a nuclear protein. It mainly contains random coil (55.13%) of which the tertiary structure was further determined with high reliability (95.76%). Cells transfected with SARS-COV-2 N protein usually show a G1/S phase block company with an increased expression of TUBA1C, TUBB6. At last, our analysis of SARS-COV-2 N protein predicted a total number of 12 phosphorylated sites and 9 potential protein kinases which would significantly affect SARS-COV-2 N protein function. Conclusion In this study, we report the physicochemical properties, subcellular localization, and biological function of SARS-COV-2 N protein. The 12 phosphorylated sites and 9 potential protein kinase sites in SARS-COV-2 N protein may serve as promising targets for drug discovery and development for of a recombinant virus vaccine.

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Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of the SARS-COV-2 nucleocapsid protein

Gao

Liu

et al. 2021

BMC Microbiol

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“…Previous studies have also reported that TUBA1C is associated with cell proliferation, and it also regulates cell cycle in many types of cancers. Furthermore, the expression of TUBA1C was reported to be correlated with p53 expression in pancreatic ductal adenocarcinoma [ 12 ]. Previous studies have reported that tubulin regulates cell metabolism and glucose stress response, reducing the dependence of cells on glycolysis.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, α-tubulin is also associated with the development of astrocytoma and chemotherapeutic resistance of liver cancer [ 11 ]. Moreover, TUBA1C is a subtype of α-tubulin, and its overexpression is associated with the poor prognosis of hepatocellular carcinoma (HCC) and pancreatic ductal adenocarcinoma [ 12 , 13 ]. However, no previous study has elucidated how the overexpression of TUBA1C affects LUAD patients.…”

Section: Introductionmentioning

confidence: 99%

Prognostic biomarker TUBA1C is correlated to immune cell infiltration in the tumor microenvironment of lung adenocarcinoma

et al. 2021

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Background TUBA1C is a microtubule component that is involved in a variety of cancers. Our main objective was to investigate TUBA1C expression, its prognostic value, its potential biological functions, and its impact on the immune system of patients with lung adenocarcinoma (LUAD). Methods The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA) and Immunohistochemistry Analysis were used to analyze TUBA1C expression, its clinicopathology, overall survival (OS), and disease-free survival (DFS) in LUAD patients. We also determined the correlation between TUBA1C and tumor-infiltrating immune cells (TIICs) by using CIBERSORT and GEPIA databases. To determine the expression of TUBA1C in LUAD, we analyzed a collection of immune infiltration levels and cumulative survival of LUAD tissues in TIMER database. By using UALCAN, STRING, and GeneMANIA databases, we investigated the protein-coding genes related to TUBA1C and its co-expression genes in LUAD tissues. Gene set enrichment analysis (GSEA) was performed by using the TCGA dataset. Results The mRNA and the protein expression of TUBA1C were found to be up-regulated in LUAD tissues. The univariate analysis indicated that an increased expression of TUBA1C was significantly correlated to the following parameters: age, stage, and lymph node metastasis. An over-expression of TUBA1C was associated with a poor prognosis of LUAD. In TIMER and CIBERSORT databases, we found that TUBA1C is correlated with 13 types of TIICs: activated B cell, activated CD4 T cell, central memory CD4 T cell, effector memory CD8 T cell, eosinophils, immature B cell, gamma-delta T cell, immature dendritic cell, mast cell, memory B cell, natural killer T cell, regulatory T cell, and type 2T helper cell. By performing GSEA, we found that TUBA1C is closely correlated to cell cycle, p53 signaling pathway, glycolysis, and gluconeogenesis. Conclusions Our findings indicate that TUBA1C is associated with TIICs in tumor microenvironment. Therefore, it serves as a novel prognostic biomarker and a target for future treatment methods of LUAD.

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“…ST6GALNAC2 has functions in the PI3K/AKT pathway (Ren et al, 2014), which is involved in many cellular processes, including proliferation, differentiation, apoptosis, cell cycle progression, and cell motility (Bellacosa et al, 2005;Engelman et al, 2006). SLC25A4 has been implicated to have many transport functions for various molecules on the mitochondrial membrane, including ATP/ADP and amino acids, which are molecules constituting cellular energy sources (Albahde et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Role of Sulfur Metabolism Gene and High-Sulfur Gene Expression in Wool Growth Regulation in the Cashmere Goat

Chai

Sun

Liu³

et al. 2021

Front. Genet.

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Sulfur, an essential mineral element for animals, mainly exists in the form of organic sulfur-containing amino acids (SAAs), such as cystine, methionine, and cysteine, within the body. The content, form, and structure of sulfur play an important role in determining the wool fiber quality. In addition, keratin-associated proteins, one of the most crucial wool fiber components, are rich in SAAs. However, sulfur metabolism from the blood to the skin and hair follicles remains unclear. In this study, we analyzed high-sulfur protein gene and sulfur metabolism genes in the cashmere goat and explored the effects of melatonin on their expression. In total, 53 high-sulfur protein genes and 321 sulfur metabolism genes were identified. We found that high-sulfur protein genes were distributed in the 3–4 and 144M regions of chromosome 1 and the 40–41M region of chromosome 19 in goats. Moreover, all year round, allele-specific expression (ASE) is higher in the 40–41M region of chromosome 19 than in the other regions. Total of 47 high-sulfur protein genes showed interaction with transcription factors and cofactors with ASE. These transcription factors and cofactors were inhibited after melatonin implantation. The network analysis revealed that melatonin may activate the sulfur metabolism process via the regulation of the genes related to cell energy metabolism and cell cycle in the skin, which provided sufficient SAAs for wool and cashmere growth. In conclusion, our findings provide a new insight into wool growth regulation by sulfur metabolism genes and high-sulfur protein genes in cashmere goats.

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Upregulated Expression of TUBA1C Predicts Poor Prognosis and Promotes Oncogenesis in Pancreatic Ductal Adenocarcinoma via Regulating the Cell Cycle

Cited by 29 publications

References 35 publications

Identification and functional analysis of the SARS-COV-2 nucleocapsid protein

Identification and functional analysis of the SARS-COV-2 nucleocapsid protein

Prognostic biomarker TUBA1C is correlated to immune cell infiltration in the tumor microenvironment of lung adenocarcinoma

Role of Sulfur Metabolism Gene and High-Sulfur Gene Expression in Wool Growth Regulation in the Cashmere Goat

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