UPLC–Q-TOF/MS-based untargeted metabolomics coupled with chemometrics approach for Tieguanyin tea with seasonal and year variations

Zhou, Peng; Hu, Ou; Fu, Haiyan; Ouyang, Li-Qun; Gong, Xuedong; Meng, Ping; Wang, Zheng; Dai, Ming; Guo, Xiaoming; Wang, Ying

doi:10.1016/j.foodchem.2019.01.050

Cited by 84 publications

(35 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a first step in assessing how tea metabolites are related to diverse genetic backgrounds, we sought to expand our knowledge of tea metabolites by untargeted metabolomic analysis. Previous untargeted analyses were limited to processed tea products or fresh leaves of a small group of closely related tea cultivars 20 , 26 , 28 , 30 , 47 , 48 . We anticipate that many tea metabolites were missed in these analyses.…”

Section: Resultsmentioning

confidence: 99%

Metabolite signatures of diverse Camellia sinensis tea populations

et al. 2020

View full text Add to dashboard Cite

The tea plant (Camellia sinensis) presents an excellent system to study evolution and diversification of the numerous classes, types and variable contents of specialized metabolites. Here, we investigate the relationship among C. sinensis phylogenetic groups and specialized metabolites using transcriptomic and metabolomic data on the fresh leaves collected from 136 representative tea accessions in China. We obtain 925,854 high-quality single-nucleotide polymorphisms (SNPs) enabling the refined grouping of the sampled tea accessions into five major clades. Untargeted metabolomic analyses detect 129 and 199 annotated metabolites that are differentially accumulated in different tea groups in positive and negative ionization modes, respectively. Each phylogenetic group contains signature metabolites. In particular, CSA tea accessions are featured with high accumulation of diverse classes of flavonoid compounds, such as flavanols, flavonol mono-/di-glycosides, proanthocyanidin dimers, and phenolic acids. Our results provide insights into the genetic and metabolite diversity and are useful for accelerated tea plant breeding.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolite signatures of diverse Camellia sinensis tea populations

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Tieguanyin is a typical oolong tea, and its production process also determines that it belongs to the category of lightly fermented oolong tea. Among the large number of different teas in China, Tieguanyin is very popular, even in East and Southeast Asia (Li, Lei, Yang, & Liu, ; Zhou et al, ).…”

Section: Discussionmentioning

confidence: 99%

Chemical characterization and bioactivity of phenolics from Tieguanyin oolong tea

et al. 2019

View full text Add to dashboard Cite

Phenolics are the main bioactive components in tea and greatly contribute to human health. Three phenolic‐enriched extracts, the ethyl acetate fraction (TEF), n‐butanol fraction (TBF), and water fraction (TWF), were obtained from Tieguanyin oolong tea, which is considered a typical type of semi‐fermented tea. The chemicals in the extracts and their antioxidant activity and cytotoxicity against 4T1 breast cancer cells were investigated in the present work. TEF was found to have the highest contents of phenolics, flavonoids, procyanidins, sugars, and catechin monomers. Meanwhile, TEF exhibited the strongest antioxidant capacity, which may be due to its abundant bioactive compounds, as validated by Pearson correlation and hierarchical clustering analysis. Furthermore, TEF showed greater inhibition of the growth of 4T1 murine breast cancer cells than TBF and TWF. Practical applications Fermentation during the processing of oolong tea causes many alterations in polyphenols, leading to different bioactivities. In the present work, three phenolic‐enriched extracts, the ethyl acetate fraction (TEF), n‐butanol fraction (TBF), and water fraction (TWF), were obtained from Tieguanyin oolong tea. Further tests showed that TEF and TBF from Tieguanyin oolong tea possessed remarkable antioxidant activity and inhibitory potential inhibition of the growth of 4T1 murine breast cancer cells in vitro due to their main bioactive compounds, including phenolics and flavonoids. Thus, the phenolic‐enriched extracts from Tieguanyin tea are expected to have a potential application in the food and pharmaceutical industries after further study.

show abstract

“…These representative types of tea contain substantial amounts of phenolic compounds, of which catechins, theaflavins, flavonols, and flavones account for approximately 15%, 4%, 0.4%, and 0.1% of the dry weight (DW), respectively [ 1 , 2 ]. One study simultaneously analyzed over 45 compounds in tea, including catechins and flavonols, using ultra high-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) [ 3 ]. Recently, 145 compounds such as hydroxycinnamic acids, catechins, and flavonols in GT and pu-erh tea were further characterized using UPLC-Q-Orbitrap-MS/MS in order to discriminate the tea sources by matching the chemical profiles [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Advanced analytical instruments and methods, such as high-resolution MS, chemometric analysis, and targeted or non-targeted metabolomics approaches, have provided a wealth of information on the potential health benefits of bioactive phenolics in agricultural plant resources [ 3 , 5 , 6 ]. Several reports suggested that phenolic compounds in GT and processed teas have anticancer, anti-inflammatory, anti-obesity, and/or antioxidative effects [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chemometric Analysis of Extracts and Fractions from Green, Oxidized, and Microbial Fermented Teas and Their Correlation to Potential Antioxidant and Anticancer Effects

Rha

Jung

Lee³

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Previous reports on phytochemicals in green tea (GT) and processed teas mainly focused on more representative compounds such as catechins. Here, we focus on the insignificantly studied non-catechin components in tea extracts, and explore the multivariate correlation between diverse phenolic compounds in tea and the in vitro antioxidant and anticancer effects. Extracts from GT and four types of processed teas were further divided into hydrophilic and hydrophobic fractions, whose phenolic compositions and antioxidant capacities were quantified using HPLC-MS and three antioxidant assays, respectively. For three types of teas, the anticancer effects of their extracts and fractions were assessed using cancer cell lines. The hydrophobic fractions had lower antioxidant capacities than the corresponding hydrophilic fractions, but exhibited superior antiproliferative effects on cancer cells compared with the whole extract and the hydrophilic fraction. Partial least squares-discriminant analysis revealed a strong correlation between the anticancer effects and the theaflavins and flavonols. Therefore, in addition to catechins, the hydrophobic fraction of tea extracts may have beneficial health effects.

show abstract

UPLC–Q-TOF/MS-based untargeted metabolomics coupled with chemometrics approach for Tieguanyin tea with seasonal and year variations

Cited by 84 publications

References 38 publications

Metabolite signatures of diverse Camellia sinensis tea populations

Metabolite signatures of diverse Camellia sinensis tea populations

Chemical characterization and bioactivity of phenolics from Tieguanyin oolong tea

Chemometric Analysis of Extracts and Fractions from Green, Oxidized, and Microbial Fermented Teas and Their Correlation to Potential Antioxidant and Anticancer Effects

Contact Info

Product

Resources

About