UPLC–PDA‐ESI–QTOF–MS/MS and GC‐MS analysis of Iranian <i>Dracocephalum moldavica</i> L.

Fattahi, Azin; Shakeri, Abolfazl; Tayarani‐Najaran, Zahra; Kharbach, Mourad; Segers, Karen; Heyden, Yvan Vander; Taghizadeh, Seyedeh Faezeh; Rahmani, Hanieh; Asili, Javad

doi:10.1002/fsn3.2396

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…Similarly, a phenolic acid cinnamoyl glucose 9 was found at m/z 309.09. Compound 1 showed peaks at m/z 127.03 and had a molecular formula of C 6 H 6 O 3 ; thus, it was identified as pyrogallol, consistent with previous reports (Cunha et al, 2017;Balkrishna et al, 2021;Brito et al, 2021;Fattahi et al, 2021). This analysis tentatively identified a total of five flavonoids.…”

Section: Phytochemical Compositionsupporting

confidence: 87%

Evaluation of the anti-inflammatory, antioxidant, and cytotoxic potential of Cardamine amara L. (Brassicaceae): A comprehensive biochemical, toxicological, and in silico computational study

et al. 2023

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Introduction:Cardamine amara L. (Brassicaceae) is an important edible plant with ethnomedicinal significance. This study aimed at evaluating the phytochemical composition, anti-inflammatory, antioxidant and cytotoxicity aspects of the hydro-alcoholic extract of C. amara (HAECA).Methods: The phytochemical composition was evaluated through total phenolic contents (TPC), total flavonoid contents (TFC) determination and UPLC-QTOF-MS profiling. Anti-inflammatory evaluation of HAECA was carried out through the carrageenan induced paw edema model. Four in vitro methods were applied in the antioxidant evaluation of HAECA. MTT assay was used to investigate the toxicity profile of the species against human normal liver cells (HL7702), human liver cancer cell lines (HepG2) and human breast cancer cell lines (MCF-7). Three major compounds (Gentisic acid, skullcapflavone and conidendrine) identified in UPLC-Q-TOF-MS analysis were selected for in silico study against cyclooxygenase (COX-I and COX-II).Results and Discussion: The findings revealed that HAECA is rich in TPC (39.32 ± 2.3 mg GAE/g DE) and TFC (17.26 ± 0.8 mg RE/g DE). A total of 21 secondary metabolites were tentatively identified in UPLC-Q-TOF-MS analysis. In the MTT cytotoxicity assay, the extract showed low toxicity against normal cell lines, while significant anticancer activity was observed against human liver and breast cancer cells. The carrageenan induced inflammation was inhibited by HAECA in a dose dependent manner and showed a marked alleviation in the levels of oxidative stress (catalase, SOD, GSH) and inflammatory markers (TNF-α, IL-1β). Similarly, HAECA showed maximum antioxidant activity through the Cupric reducing power antioxidant capacity (CUPRAC) assay (31.21 ± 0.3 mg TE/g DE). The in silico study revealed a significant molecular docking score of the three studied compounds against COX-I and COX-I. Conclusively the current study encourages the use of C. amara as a novel polyphenolic rich source with anti-inflammatory and antioxidant potential and warrants further investigations on its toxicity profile.

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Section: Phytochemical Compositionsupporting

confidence: 87%

Evaluation of the anti-inflammatory, antioxidant, and cytotoxic potential of Cardamine amara L. (Brassicaceae): A comprehensive biochemical, toxicological, and in silico computational study

et al. 2023

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“…According to referenced data from the literature about DMEO chemical composition ( Table 8 ), as well as cluster analysis performed using this data for the construction an unrooted phylogenetic tree ( Figure 4 ), it could be assumed that the largest number of accessions belonged to the geranial + neral + geranyl acetate chemotype [ 2 , 9 , 10 , 12 , 17 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. The D. moldavica plants grown and utilized in this study also belonged to the above chemotype.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition, Antioxidant, and Antimicrobial Activity of Dracocephalum moldavica L. Essential Oil and Hydrolate

Aćimović

Šovljanski

Šeregelj

et al. 2022

Plants

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Steam distillation was used for the isolation of Dracocephalum moldavica L. (Moldavian dragonhead) essential oil (DMEO). This aromatic herbaceous plant is widespread across the Northern Hemisphere regions and has been utilized in health-improving studies and applications. In addition to the DMEO, the hydrolate (DMH), a byproduct of the distillation process, was also collected. The DMEO and DMH were analyzed and compared in terms of their chemical composition, as well as their in vitro biological activities. The main component in DMEO was geranyl acetate, while geranial was dominant in DMH. The DMEO demonstrated better antioxidant and antimicrobial activities compared with the DMH against Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, which represent sources of food-borne illness at the global level. The DMEO and DMH show promise as antioxidant and antimicrobial additives to various products.

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“…Peak 1 gave the mass spectrum ion at 116.0702 (C 5 H 9 NO 2 ) with MS/MS fragment of 70.0647 (C 4 H 7 N) [M+H-HCOOH] + , which was detected to be D-proline [14][15][16] [25]. Peaks 18,21,23,24,25,29,31,32,33,39,42,43,49,50,52, ] − ) were the same regular, which were identified as gallic acid [20], protocatechuic acid [23], acetylsalicylic acid [26], caftaric acid [20], gentisic acid [23], 4-hydroxybenzoic acid [27], p-coutaric acid [28], m-coumaric acid [28], coutaric acid [28], vanillic acid [27], fertaric acid [28], caffeic acid [28], desaminotyrosine [29], 2-hydroxybenzoic acid [28], 3-hydroxy-4-methoxybenzoic acid [30,31], p-coumaric acid [28], homogentisic acid [23], ellagic acid [32] and dihydrocaffeic acid [33]. Peak 79 had the major first-order mass spectrum at m/z 270.0662 (C 11 H 12 O 4 ).…”

Section: Identification Of 94 Components From Red Winementioning

confidence: 99%

“…were identified as the type of ion of 151.0037 (C 7 H 4 O 4 ), which were the characteristic fragment of flavonoid components. Indeed, they were epicatechin gallate [23], epicatechin [23], myricetin-3-O-galactoside [23], quercitrin [28], quercetin-3-O-glucuronide [28], laricitrin-3-O-glucoside [37], luteolin-3'-glucoside [23], astilbin [38], isoquercitrin [22], astragalin [38], myricetin [21], isorhamnetin-3-O-glucoside [28], quercetin [22], laricitrin [37], naringenin [23], kaempferol [22] and isorhamnetin [28]. Peak 45 had the mass spectrum ion at 197.0454 (C 9 H 10 O 5 ) with MS/MS ions at 182.0256 [M-H-…”

Section: Identification Of 94 Components From Red Winementioning

confidence: 99%

Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

Gao

Wang

et al. 2021

Molecules

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According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.

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UPLC–PDA‐ESI–QTOF–MS/MS and GC‐MS analysis of Iranian Dracocephalum moldavica L.

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 17 publications

References 33 publications

Evaluation of the anti-inflammatory, antioxidant, and cytotoxic potential of Cardamine amara L. (Brassicaceae): A comprehensive biochemical, toxicological, and in silico computational study

Evaluation of the anti-inflammatory, antioxidant, and cytotoxic potential of Cardamine amara L. (Brassicaceae): A comprehensive biochemical, toxicological, and in silico computational study

Chemical Composition, Antioxidant, and Antimicrobial Activity of Dracocephalum moldavica L. Essential Oil and Hydrolate

Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

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