UPLC-MS: An Emerging Novel Technology and Its Application in Food Safety

Abstract

Over the past decade, food safety has become an important issue worldwide due to higher incidences of food contamination. Currently, one of the great challenges in food safety is the analysis of emerging food contaminants. Moreover, the scope, relevance, and level of food safety and testing have never been in such complexity than in today's global marketplace. In recent years, a novel technology ultra performance liquid chromatography (UPLC) coupled with mass spectroscopy (MS) has been developed to estimate the food contaminants, as well as food components with better accuracy, sensitivity, precision, and high throughput. UPLC-MS works on van Deemter principle, which states that, the flow rate of smaller particles are much faster in compare with large particles as well as unfolding the correlation of flow rate and plate height. Additionally, various food components as well as food contaminants such as vitamins, amino acid, metabolite identification, adulteration, forensic testing, toxicity studies, phytoconstituents, pesticide in agricul ture, antibiotic residue, hormones, dyes and pigment analysis can be performed using UPLC-MS. Moreover, uniqueness of UPLC-MS and its wide range of application makes it an important tool for food safety laboratory around the world.

Glutathione: Role in Oxidative/Nitrosative Stress, Antioxidant Defense, and Treatments

“…[214] Unique method. [215] Estimation can be done even with lower levels of analytes. [215] Larger linear dynamic range.…”

“…[215] Estimation can be done even with lower levels of analytes. [215] Larger linear dynamic range. [216] Expensive method [216] thus cannot be used in lower budgets projects.…”

Principles and Applications of Ultra-High-Performance Liquid Chromatography

“…Food additives and mycotoxins are among the major toxic treats in different foods consumed worldwide [64]. Food stuffs are vulnerable to infection and contamination in the field or during storage.…”

“…For both qualitative and quantitative research, this novel procedure enables the isolation, purification, and detection of components from a mixture. UHPLC-MS has lately been utilized to estimate food pollutants and components in order to improve food safety [64].…”

Metabolomics as a Tool to Elucidate the Sensory, Nutritional and Safety Quality of Wheat Bread—A Review

“…However, the residues of these contaminants in food crops constitute a health risk, even if they are not permitted in higher concentrations than the limits. For detection and accurate determination of pesticides several studies proposed UHPLC-Orbitrap-MS/UPLC-MS method to detect pesticides in very low amounts from different food matrices, among which there are cereals, honey, eggs [94]. Mastovska et al [95] optimized DSI-LVI-GC-TOFMS and UPLCMS/MS to analyze 180 pesticides between cereals grain.…”