Upgrading Methylation Method for Structural Studies of Polysaccharides: Case Analysis of a Bioactive Polysaccharide from<i>Acacia tortilis</i>

Nagar, Shipra; Lakhera, Ajeet Kumar; Kumar, Vineet

doi:10.1080/22311866.2020.1767688

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…The permethylated derivatives (5 mg) were hydrolyzed with 4 mL of 2 M TFA at 120 °C for 2 h followed by evaporation to dryness. The residue was successively reduced with excess NaBH4 and acetylated with anhydride acetic/pyridine (1:1 v/v) solution at 100 °C in 20 min. − The methylated alditol acetate sugars were analyzed by using the GC–MS Shimadzu system (2010) with a DB-5 capillary, with helium as the carrier gas and a linear velocity of 2 mL/min. The temperature program used was an initial temperature of 65 °C in 1 min, increasing by 8 °C/min to 250 °C and staying in 10 min, and increasing by 2 °C/min to 280 °C and staying in 5 min.…”

Section: Methodsmentioning

confidence: 99%

Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Thuy

Tran

et al. 2020

ACS Omega

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This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated Lactobacillus plantarum W1 (EPS-W1), which belongs to genus Lactobacillus, is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopolysaccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of →6)-d-Glcp-(1→; →3)-d-Manp-(1→; →3)-d-Glcp-(1→ and a branch of →6)-d-Manp-(1→; →2)-d-Glcp-(1→. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Thuy

Tran

et al. 2020

ACS Omega

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“…The obtained sample was subjected to 2 mL of solution of anhydride acetic and pyridine (1.1, v/v) at 100 °C for 20 min and dried under a N 2 stream. The final product was redissolved in CHCl 3 for GC–MS analysis. − …”

Section: Methodsmentioning

confidence: 99%

Rich d-Fructose-Containing Polysaccharide Isolated from Myxopyrum smilacifolium Roots toward a Superior Antioxidant Biomaterial

Sơn

Nguyen

et al. 2022

ACS Omega

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The presented study attempts to unveil and evaluate the antioxidant activity of a novel heteropolysaccharide separated from the roots of Myxopyrum smilacifolium (denoted as PS-MSR). The molecular weight of PS-MSR is found to be 1.88 × 104 Da and contains two principal sugars, which are d-glucose and d-fructose, in the backbone. Decoding the structure of the obtained PS-MSR sample has disclosed a novel polysaccharide for the first time. Indeed, the PS-MSR is composed of (1 → 3)-linked glucosyl units and (2 → 3)-linked fructosyl units. In addition, the 1D and 2D NMR spectra of the PS-MSR sample display the repeating unit of the isolated polysaccharide, [→3)-α-d-Glcp-(1 → 3)-β-d-Frucf-(2 → 3)-β-d-Frucf-2 → 3)-)-β-d-Frucf-β-(2→] n . Interestingly, the PS-MSR sample exhibits outstanding antioxidant activity, signifying the potential utilization of the explored polysaccharide for antioxidant-based material.

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Unfolding the structure of polysaccharide isolated from Myxopyrum smilacifolium leaves toward a robust antioxidant

Nguyen

Sơn

et al. 2023

Bioactive Carbohydrates and Dietary Fibre

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Upgrading Methylation Method for Structural Studies of Polysaccharides: Case Analysis of a Bioactive Polysaccharide fromAcacia tortilis

Cited by 5 publications

References 25 publications

Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Rich d-Fructose-Containing Polysaccharide Isolated from Myxopyrum smilacifolium Roots toward a Superior Antioxidant Biomaterial

Unfolding the structure of polysaccharide isolated from Myxopyrum smilacifolium leaves toward a robust antioxidant

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