Decoding the Capability of <i>Lactobacillus plantarum</i> W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Thuy, Thi Bich; Tran, Bao Khanh; Tran, Thi Van Thi; Le, Trung Hieu; Cnockaert, Margo; Vandamme, Peter; Nguyen, Thi Hong Chuong; Nguyen, Chinh Chien; Hong, Sung Hwan; Kim, Soo Young; Le, Quyet Van

doi:10.1021/acsomega.0c05256

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Moreover, the presence of multiple signal peaks at δ 4.5-5.5 ppm implied that the isolated EPS was a heteropolysaccharide consisting of multiple monosaccharides. The EPS produced by L. plantarum W1 had six ectopic proton signals (δ 5.76, 5.67, 5.59, 5.59, 5.56, 5.55, 5.37 ppm) in the anomalous region (δ 4.5-5.5 ppm), showing that the EPS samples were composed of seven monosaccharides of heteropolysaccharides (Do et al, 2020), which was different from the results of this test. Therefore, the isolated EPS was inferred to be a pyranose consisting of α-(1 → 6) and α-(1 → 3) glycosidic bonds.…”

Section: Nuclear Magnetic Resonance Spectroscopy Analysiscontrasting

confidence: 85%

“…In addition, EPSs produced by some LAB are heteropolysaccharides composed of different monosaccharide structures. Do et al (2020) found that EPS-W1 produced by L. plantarum was a heteropolysaccharide composed of glucose and mannose. The SSC-12 EPS produced by Pseudomonas pentosus was a heteropolysaccharide composed of glucose, mannose, galactose, arabinose, and rhamnose ( Fan et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

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Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by Lactiplantibacillus plantarum HDC-01 isolated from sauerkraut

et al. 2023

Front. Microbiol.

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In this study, an exopolysaccharide (EPS)-producing strain of Lactiplantibacillus plantarum HDC-01 was isolated from sauerkraut, and the structure, properties and biological activity of the studied EPS were assessed. The molecular weight of the isolated EPS is 2.505 × 106 Da. Fourier transform infrared spectrometry (FT-IR) and nuclear magnetic resonance (NMR) results showed that the EPS was composed of glucose/glucopyranose subunits linked by an α-(1 → 6) glycosidic bond and contained an α-(1 → 3) branching structure. X-ray diffraction (XRD) analysis revealed the amorphous nature of the EPS. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that the isolated EPS had a smooth and compact surface with several protrusions of varying lengths and irregularly shaped material. Moreover, the studied EPS showed good thermal stability, water holding capacity, and milk coagulation ability and promoted the growth of probiotics. L. plantarum EPS may be used as prebiotics in the fields of food and medicine.

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Section: Nuclear Magnetic Resonance Spectroscopy Analysiscontrasting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by Lactiplantibacillus plantarum HDC-01 isolated from sauerkraut

et al. 2023

Front. Microbiol.

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“…It turns out that Fraction I, which contained the highest amount of neutral carbohydrates (96%), was concentrated, lyophilized, and purified on a Sephadex G-100 column (10 mm × 600 mm) before being eluted with deionized water, NaCl 0.1 M, and NaCl 0.3 M eluents (flow rate, 0.2 mL/min), respectively. Finally, the achieved PS was obtained for further investigations. , …”

Section: Methodsmentioning

confidence: 99%

“…Finally, the achieved PS was obtained for further investigations. 30,31 2.4. Determination of Molecular Mass.…”

Section: Extraction Of Psmentioning

confidence: 99%

Rich d-Fructose-Containing Polysaccharide Isolated from Myxopyrum smilacifolium Roots toward a Superior Antioxidant Biomaterial

Sơn

Nguyen

et al. 2022

ACS Omega

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The presented study attempts to unveil and evaluate the antioxidant activity of a novel heteropolysaccharide separated from the roots of Myxopyrum smilacifolium (denoted as PS-MSR). The molecular weight of PS-MSR is found to be 1.88 × 104 Da and contains two principal sugars, which are d-glucose and d-fructose, in the backbone. Decoding the structure of the obtained PS-MSR sample has disclosed a novel polysaccharide for the first time. Indeed, the PS-MSR is composed of (1 → 3)-linked glucosyl units and (2 → 3)-linked fructosyl units. In addition, the 1D and 2D NMR spectra of the PS-MSR sample display the repeating unit of the isolated polysaccharide, [→3)-α-d-Glcp-(1 → 3)-β-d-Frucf-(2 → 3)-β-d-Frucf-2 → 3)-)-β-d-Frucf-β-(2→] n . Interestingly, the PS-MSR sample exhibits outstanding antioxidant activity, signifying the potential utilization of the explored polysaccharide for antioxidant-based material.

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“…Soybean whey, as a byproduct produced of L. plantarum W1 named EPS-W1 marked by phenylalanyl-tRNA sequencing method using a set of primers for PCR amplification, shows remarkable properties in production aspects, such as in functional foods, food industries, and biomedical applications [119]. Some other LAB spp.…”

Section: Lactobacillus Eps As An Immunomodulatory Agentmentioning

confidence: 99%

Recent Advances in the Production of Exopolysaccharide (EPS) from Lactobacillus spp. and Its Application in the Food Industry: A Review

et al. 2021

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Exopolysaccharide (EPS) show remarkable properties in various food applications. In this review paper, EPS composition, structural characterization, biosynthesis pathways, and recent advancements in the context of application of EPS-producing Lactobacillus spp. in different food industries are discussed. Various chemical and physical properties of Lactobacillus EPS, such as the structural, rheological, and shelf-life enhancement of different food products, are mentioned. Moreover, EPSs play a characteristic role in starter culture techniques, yogurt production, immunomodulation, and potential prebiotics. It has been seen that the wastes of fermented and non-fermented products are used as biological food for EPS extraction. The main capabilities of probiotics are the use of EPS for technological properties such as texture and flavor enhancement, juiciness, and water holding capacities of specific food products. For these reasons, EPSs are used in functional and fermented food products to enhance the healthy activity of the human digestive system as well as for the benefit of the food industry to lower product damage and increase consumer demand. Additionally, some pseudocereals such as amaranth and quinoa that produce EPS also play an important role in improving the organoleptic properties of food-grade products. In conclusion, more attention should be given to sustainable extraction techniques of LAB EPS to enhance structural and functional use in the developmental process of food products to meet consumer preferences.

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Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Cited by 8 publications

References 34 publications

Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by Lactiplantibacillus plantarum HDC-01 isolated from sauerkraut

Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by Lactiplantibacillus plantarum HDC-01 isolated from sauerkraut

Rich d-Fructose-Containing Polysaccharide Isolated from Myxopyrum smilacifolium Roots toward a Superior Antioxidant Biomaterial

Recent Advances in the Production of Exopolysaccharide (EPS) from Lactobacillus spp. and Its Application in the Food Industry: A Review

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