Updating of the correlation between lpELISA titers and protection from virus challenge for the assessment of the potency of polyvalent aphtovirus vaccines in Argentina

Maradei, Eduardo; Torre, J.L. La; Robiolo, Blanca; Esteves, J; Seki, Cristina; Pedemonte, Andrea; Iglesias, Marcela; D’Aloia, Ricardo; Mattion, Nora

doi:10.1016/j.vaccine.2008.09.033

Cited by 58 publications

(71 citation statements)

References 25 publications

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“…Not only were the LPB-ELISA results more capable to distinguish between vaccines, they were also more reproducible, as they are not influenced by variations in tissue culture susceptibility. Although neutralizing antibodies are often considered in relation to protection, LPB-ELISA titers are also correlated with protection (36,37). Therefore, the LPB-ELISA seems to perform better than the neutralization tests to discriminate between vaccines.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

Tekleghiorghis¹,

Weerdmeester²,

Hemert-Kluitenberg³

et al. 2014

Clin. Vaccine Immunol.

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Vaccination has been one of the most important interventions in disease prevention and control. The impact of vaccination largely depends on the quality and suitability of the chosen vaccine. To determine the suitability of a vaccine strain, antigenic matching is usually studied by in vitro analysis. In this study, we performed three in vitro test methods to determine which one gives the lowest variability and the highest discriminatory capacity. Binary ethylenimine inactivated vaccines, prepared from 10 different foot-and-mouth disease (FMD) virus serotype A strains, were used to vaccinate cattle (5 animals for each strain). The antibody titers in blood serum samples 3 weeks postvaccination (w.p.v.) were determined by a virus neutralization test, neutralization index test, and liquid-phase blocking enzyme-linked immunosorbent assay (ELISA). The titers were then used to calculate relationship coefficient (r 1 ) values. These r 1 values were compared to the genetic lineage using receiver operating characteristic (ROC) analysis. In the two neutralization test methods, the median titers observed against the test strains differed considerably, and the sera of the vaccinated animals did not always show the highest titers against their respective homologous virus strains. When the titers were corrected for test strain effect (scaling), the variability (standard error of the mean per vaccinated group) increased because the results were on a different scale, but the discriminatory capacity improved. An ROC analysis of the r 1 value calculated on both observed and scaled titers showed that only r 1 values of the liquid-phase blocking ELISA gave a consistent statistically significant result. Under the conditions of the present study, the liquid-phase blocking ELISA showed less variation and still had a higher discriminatory capacity than the other tests.

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Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that there is a strong relationship between antibody response and protection (36)(37)(38). The precision of this relationship can be improved slightly by including other immunological techniques (39,40).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

Tekleghiorghis¹,

Weerdmeester²,

Hemert-Kluitenberg³

et al. 2014

Clin. Vaccine Immunol.

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“…Such cutoff values are particular for each strain and were calculated from correlations involving large numbers of animals, combining results of in vivo challenge tests and in vitro serology assays (26,27). This concept, however, diminishes the importance of other relevant aspects of immunity, such as the respiratory mucosal response that may play an important role during aerogenous FMDV infection in cattle.…”

Section: Discussionmentioning

confidence: 99%

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Pega

Giacomo

Bucafusco

et al. 2015

J Virol

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Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the prescapular lymph node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in prescapular LN, while FMDV-specific ASC were detected in all lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals (n ‫؍‬ 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virusspecific B lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective virus. F oot-and-mouth disease (FMD) is a highly contagious and acute viral disease affecting a wide range of economically important livestock species (1). All domestic biungulates are susceptible to infection with the FMD virus (FMDV); in addition, a number of wildlife species may act as reservoirs for the virus under particular ecological conditions (2). Lethality has been described for young animals and certain FMDV strains (3). However, the main disruptive potential of this transboundary disease is the high morbidity rate and the numerous indirect losses associated with its incursions into territories with susceptible populations. Consequently, FMD outbreaks may result in severe and far-reaching economic losses due to the interruption of regional and international trade in developed countries (4, 5), but also importantly, due to the loss of animals, production efficiency, and genetic diversity in developing regions (6).Cattle are highly susceptible to FMDV, and virus usually gains entry through the respiratory tract of the animals (3). The soft palate and pharynx were identified as primary sites of FMDV replication and persistence in bovines infected through the oronasal route (7,8). FMDV infection progresses through replication in pneumocytes, permitting the virus to accomplish an extensi...

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“…In South America, a cut-off of a 75% (EPP-75%) is used to estimate protective vaccine-induced responses, statistically predicted from challenge experiments in cattle, using either VNT or LPBE titres. These cuts-off values are considered when assessing herd immunity (Maradei et al 2008;Robiolo et al 2010b).…”

Section: Introductionmentioning

confidence: 99%

“…LPBE has been validated for bovine samples, as being as reliable and precise as the VNT (Robiolo et al 1995;Maradei et al 2008;Robiolo et al 2010b) and is routinely used to assess herd immunity (Leon et al 2014). A single-dilution LPBE-Mab has also been validated for the four vaccine strains included in the Argentinean commercial vaccine (Robiolo et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis)

Sala

Trotta

Mansilla

et al. 2017

Journal of Applied Animal Research

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Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using the same Enzyme-linked immunosorbent assays (ELISAs) applied for bovine sera, assuming an agreement between the ELISA's diagnostic results and those obtained using the virus neutralization test (VNT). Here we evaluated the accuracy of different ELISA tests to assess vaccine-induced antibodies against FMDV in buffalo's sera classified according to their VNT titres. Currently used liquid-phase blocking ELISA yielded very low specificity, producing high titres for many samples with low VNT titres. To increase specificity, we developed an indirect ELISA using purified 140S viral particles and an avidity single-dilution ELISA, which includes a urea washing step after the incubation of the diluted serum sample, to detach weak binders. Combining these two highthroughput single-dilution tests, an excellent concordance with VNT was achieved. This is the first study analysing the diagnostic agreement of traditional and novel serological tests with VNT for the indirect assessment of antibodies against FMDV capsid proteins in buffalo serum samples. ARTICLE HISTORY

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Updating of the correlation between lpELISA titers and protection from virus challenge for the assessment of the potency of polyvalent aphtovirus vaccines in Argentina

Cited by 58 publications

References 25 publications

Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis)

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