Updated taxonomy on Gerronema (Porotheleaceae, Agaricales) with three new taxa and one new record from China

Abstract

Only three Gerronema (Porotheleaceae) species have been previously recorded in China. Here, we report collections of a fourth species in China: G. nemorale Har. Takah., which is widely distributed in Chinese temperate to subtropical zones. We also formally describe three new species, collected from Anhui, Fujian, and Zhejiang provinces: G. baishanzuensesp. nov., G. microcarpumsp. nov., and G. zhujiansp. nov. Furthermore, we include the results of a phylogenetic analysis of Porotheleaceae based on a multi-locus (ITS + nLSU) dataset. The results, which indicate that Gerronema is polyphyletic, support the taxonomic recognition of the three new species. Morphological descriptions, photographs, line drawings, and comparisons with closely related taxa are presented for the new and newly recorded species. A key to the seven species of Gerronema in China is also provided.

Four new species of Mycena sect. Calodontes (Agaricales, Mycenaceae) from northeast China

“…where a-c represent a basidiospores of b basidiomata from c specimens measured; d and h are the minimum and maximum length (5% extremum), respectively, e and g indicate the range of values for the remaining 90% of the spores and f is the average length; width (i-m) and Q values (n-q) are expressed in a similar manner; and r and s are the average Q value and its standard deviation, respectively (Ge et al 2021;Liu et al 2021;Na et al 2021;Na et al 2022). The measurement of basidia, cystidia and other characters were each based on 20 observations.…”

“…The PCR reactions were performed in a total volume of 25 μl containing 2 μl DNA template, 1 μl for each primer, 8.5 μl nuclease-free H 2 O and 12.5 μl 2× Utaq PCR MasterMix (ZomanBio, Beijing, China). The PCR protocol for amplification of the ITS region was as follows: 94 °C for 4 min, then 34 cycles of 94 °C for 45 s, 52 °C for 45 s and 72 °C for 1 min, with a final extension of 72 °C for 10 min (Na et al 2022). The PCR protocol for amplification of the rpb1 and tef1 regions followed that of Harder et al (2013): 94 °C for 60 s, then 10 cycles of 94 °C for 35 s, 53 °C for 45 s and 72 °C for 45 s; then 25 cycles of 94 °C for 35 s, 56 °C for 45 s, 72 °C for 45 s and final extension of 72 °C for 10 min.…”

Eoscyphella luciurceolata gen. and sp. nov. (Agaricomycetes) Shed Light on Cyphellopsidaceae with a New Lineage of Bioluminescent Fungi

“…From previous phylogenetic analyses, G. viridilucens has been proposed within Porotheleaceae [70,71]. However, our LSU rDNA phylogram (Figure 1b…”

“…Existing sequences of known bioluminescent fungi were also downloaded from GenBank to compose a final dataset that includes all known bioluminescent and cyphelloid lineages. The LSU rDNA sequence most likely misnamed G. viridilucens (EF514207), which is available on GenBank and was used in the phylogenetic analyses by Vizzini et al [70] and Na et al [71], is 99.7% identical to sequence M. lucentipes (DED7828) [72]. For this reason, we excluded the sequence EF514207 from our phylogenetic analyses and included a new one of G. viridilucens (DED7822), originally from type locality and morphologically described and confirmed [22].…”

 Pseudosperma arenarium (Inocybaceae), a new poisonous species from Eurasia, based on morphological, ecological, molecular and biochemical evidence

“…The number of measured basidiospores is given as an abbreviation [n/m/p], which denotes n spores measured from m basidiomata of p collections. The measurements and Q values are given as (a)b-c(d), "b-c" covers a minimum of 90% of the measured values, "a" and "d" represent the extreme values; Q means the ratio of length/width in an individual basidiospore, Q m is the average Q of all basidiospores ± sample standard deviation (Ge et al 2021;Na et al 2022). Colour designations follow Kornerup and Wanscher (1978).…”