Update On the Frequency of Ile1016 Mutation In Voltage-Gated Sodium Channel Gene of Aedes aegypti In Mexico

Siller, Quetzaly; Ponce, Gustavo; Lozano, Saul; Flores, Adriana E.

doi:10.2987/11-6149.1

Cited by 28 publications

(21 citation statements)

References 57 publications

(30 reference statements)

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“…The presence of the I1,016 mutation in Colombia provides the first evidence of another mechanism of resistance to pyrethroids, in addition to the enzymatic alteration previously described in different populations of other regions of Colombia [5,15,29]. We found relatively low I1,016 homozygote frequencies as compared to other pyrethroid resistant populations from other countries [28,[39][40][41][42]. Saavedra-Rodriguez et al [28], observed that 57.4% of I1,106/ V1016 heterozygotes knocked down at 1 h of exposure to permethrin eventually recovered at 24 h. Thus the rate of recovery following knockdown is inherited as an additive genetic trait with heterozygotes exhibiting an intermediate phenotype which may allow populations to more quickly respond to selection pressure.…”

Section: Discussionmentioning

confidence: 86%

Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Maestre‐Serrano¹,

Gómez‐Camargo²,

Ponce‐García³

et al. 2014

Pesticide Biochemistry and Physiology

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Section: Discussionmentioning

confidence: 86%

Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Maestre‐Serrano¹,

Gómez‐Camargo²,

Ponce‐García³

et al. 2014

Pesticide Biochemistry and Physiology

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“…At present, no other report of this point mutation in another Southeast Asian country has been published. The V1016I and I1011 (M or V) mutations seem to be widely distributed with high frequency in Latin American countries [7], [11], [18]–[21]. It is interesting to speculate why the V1016G point mutation occurs with high frequency in Myanmar and Thailand.…”

Section: Discussionmentioning

confidence: 99%

Co-occurrence of Point Mutations in the Voltage-Gated Sodium Channel of Pyrethroid-Resistant Aedes aegypti Populations in Myanmar

et al. 2014

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BackgroundSingle amino acid substitutions in the voltage-gated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance in insects. The kdr gene has been observed in several mosquito species; however, point mutations in the para gene of Aedes aegypti populations in Myanmar have not been fully characterized. The aim of the present study was to determine the types and frequencies of mutations in the para gene of Aedes aegypti collected from used tires in Yangon City, Myanmar.Methodology/Principal FindingsWe determined high pyrethroid resistance in Aedes aegypti larvae at all collection sites in Yangon City, by using a simplified knockdown bioassay. We showed that V1016G and S989P mutations were widely distributed, with high frequencies (84.4% and 78.8%, respectively). By contrast, we were unable to detect I1011M (or I1011V) or L1014F mutations. F1534C mutations were also widely distributed, but with a lower frequency than the V1016G mutation (21.2%). High percentage of co-occurrence of the homozygous V1016G/S989P mutations was detected (65.7%). Additionally, co-occurrence of homozygous V1016G/F1534C mutations (2.9%) and homozygous V1016G/F1534C/S989P mutations (0.98%) were detected in the present study.Conclusions/SignificancePyrethroid insecticides were first used for malaria control in 1992, and have since been constantly used in Myanmar. This intensive use may explain the strong selection pressure toward Aedes aegypti, because this mosquito is generally a domestic and endophagic species with a preference for indoor breeding. Extensive use of DDT for malaria control before the use of this chemical was banned may also explain the development of pyrethroid resistance in Aedes aegypti.

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“…High frequencies of this resistance allele were subsequently found in collections of Ae. aegypti from 78 sites in Mexico with some of the highest frequencies detected in collections from Veracruz state (Ponce et al 2009, Siller et al 2011). …”

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Wide Spread Cross Resistance to Pyrethroids in <I>Aedes aegypti</I> (Diptera: Culicidae) From Veracruz State Mexico

et al. 2013

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Seven F1 strains of Aedes aegypti (L.) were evaluated by bottle bioassay for resistance to the pyrethroids d-phenothrin, permethrin, deltamethrin, λ-cyalothrin, bifenthrin, cypermethrin, α-cypermethrin and z-cypermethrin. The New Orleans strain was used as a susceptible control. Mortality rates after a 1h exposure and following a 24h recovery period were determined. The resistance ratio between the 50% knockdown values (RRKC50) of the F1 and New Orleans strains indicated high levels of knockdown resistance (kdr). The RRKC50 with α-cypermethrin varied from 10–100 among strains indicating high levels of kdr. Most of the strains had moderate resistance to d-phenothrin. Significant but much lower levels of resistance were detected for λ–cyalothrin, permethrin and cypermethrin. For z-cypermethrin and bifenthrin, only one strain exhibited resistance with RRKC50 values of 10- and 21-fold, respectively. None of the strains showed RRKC50 >10 with deltamethrin, and moderate resistance was seen in three strains, while the rest were susceptible. Mosquitoes from all strains exhibited some recovery from all pyrethroids except d-phenothrin. Regression analysis was used to analyze the relationship between RRLC50 and RRKC50. Both were highly correlated (R2 = 0.84 – 0.97) so that the slope could be used to determine how much additional pyrethroid was needed to insure lethality. Slopes ranged from 0.875 for d-phenothrin (RRLC50 ≃ RRKC50) to 8.67 for λ–cyalothrin (~8.5 fold more insecticide needed to kill). Both RRLC50 and RRKC50 values were highly correlated for all pyrethroids except bifenthrin indicating strong cross resistance. Bifenthrin appears to be an alternative pyrethroid without strong cross resistance that could be used as an alternative to the current widespread use of permethrin in Mexico.

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Update On the Frequency of Ile1016 Mutation In Voltage-Gated Sodium Channel Gene of Aedes aegypti In Mexico

Cited by 28 publications

References 57 publications

Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Co-occurrence of Point Mutations in the Voltage-Gated Sodium Channel of Pyrethroid-Resistant Aedes aegypti Populations in Myanmar

Wide Spread Cross Resistance to Pyrethroids in <I>Aedes aegypti</I> (Diptera: Culicidae) From Veracruz State Mexico

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