Update in Tuberculosis 2006

Yew, Wing Wai; Leung, Chi Chiu

doi:10.1164/rccm.200611-1653up

Cited by 16 publications

(5 citation statements)

References 51 publications

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“…T cell IFN-c release assays (TIGRA) are more specific and sensitive diagnostic tools for MTB infection than the tuberculin skin test (TST) [26,27,28]. TIGRA measures IFN-c production by mononuclear cells following stimulation with restricted antigens that are absent from BCG or most non-tuberculous Mycobacteria (NTM) [1].…”

Section: Discussionmentioning

confidence: 99%

Identification of Mycobacterium tuberculosis-Specific Th1, Th17 and Th22 Cells Using the Expression of CD40L in Tuberculous Pleurisy

Qiao

et al. 2011

PLoS ONE

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Important advances have been made in the immunodiagnosis of tuberculosis (TB) based on the detection of Mycobacterium tuberculosis (MTB)-specific T cells. However, the sensitivity and specificity of the immunological approach are relatively low because there are no specific markers for antigen-specific Th cells, and some of the Th cells that do not produce cytokines can be overlooked using this approach. In this study, we found that MTB-specific peptides of ESAT-6/CFP-10 can stimulate the expression of CD40L specifically in CD4+ T cells but not other cells from pleural fluid cells (PFCs) in patients with tuberculous pleurisy (TBP). CD4+CD40L+ but not CD4+CD40L− T cells express IFN-γ, IL-2, TNF-α, IL-17 or IL-22 after stimulation with MTB-specific peptides. In addition, CD4+CD40L+ T cells were found to be mostly polyfunctional T cells that simultaneously produce IFN-γ, IL-2 and TNF-α and display an effector or effector memory phenotype (CD45RA−CD45RO+CCR7−CD62L−ICOS−). To determine the specificity of CD4+CD40L+ T cells, we incubated PFCs with ESTA-6/CFP-10 peptides and sorted live CD4+CD40L+ and CD4+CD40L− T cells by flow cytometry. We further demonstrated that sorted CD4+CD40L+, but not CD4+CD40L− fractions, principally produced IFN-γ, IL-2, TNF-α, IL-17 and IL-22 following restimulation with ESTA-6/CFP-10 peptides. Taken together, our data indicate that the expression of CD40L on MTB-specific CD4+ T cells could be a good marker for the evaluation and isolation of MTB-specific Th cells and might also be useful in the diagnosis of TB.

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Section: Discussionmentioning

confidence: 99%

Identification of Mycobacterium tuberculosis-Specific Th1, Th17 and Th22 Cells Using the Expression of CD40L in Tuberculous Pleurisy

Qiao

et al. 2011

PLoS ONE

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“…CD4 1 CD25 high regulatory T cells are thought to modify this process and are increased in number. 16,18 The transcription factor FoxP3 helps to upregulate the numbers of CD25 and cytotoxic T lymphocyte-associated antigen 4 receptors on T regulatory cells. It has been reported that FoxP3 mRNA expression is increased, which is consistent with T regulatory cell suppression or modification of the T H 1 response in tuberculosis.…”

Section: Granulomatous T H 1 Inflammatory Conditionsmentioning

confidence: 99%

“…It has been reported that FoxP3 mRNA expression is increased, which is consistent with T regulatory cell suppression or modification of the T H 1 response in tuberculosis. 16,18 …”

Section: Granulomatous T H 1 Inflammatory Conditionsmentioning

confidence: 99%

7. Immunologic lung disease

Greenberger

2008

Journal of Allergy and Clinical Immunology

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“…TIGRA have been compared to the TST in a number of studies and proven to be superior to the TST in terms of sensitivity (TST 55%-74%, TIGRA 83%-97%) and specificity (TST 56%-98%, TIGRA 92%-100%) [2][3][4]. The advantages of the new tests are the availability of the test result within 24 h independent of a patient return visit, the absence of any influence of prior BCG vaccination, and a lower cross reactivity in cases of NTM infections.…”

Section: T Cell Interferon-c Release Assaysmentioning

confidence: 99%

Suttons’s Law: Local Immunodiagnosis of Tuberculosis

Jafari

Lange

2008

Infection

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Recently, important advances have been made in the immunodiagnosis of tuberculosis. New T cell interferon-gamma release assays (TIGRA) are more specific and more sensitive than the tuberculin skin test (TST) for the diagnosis of Mycobacterium tuberculosis (MTB) infection. However, like the TST, TIGRA are unable to distinguish between active tuberculosis (TB), latent TB infection (LTBI) and treated TB if performed on blood mononuclear cells alone. In active TB, MTB-specific T cells are actively recruited to the site of infection and can rapidly be identified in extrasanguinous fluids, such as pleural effusions, ascites, cerebrospinal fluid, and in bronchoalveolar lavages. This review summarizes recent findings comparing systemic and local immune responses against MTB. Although bacteriological and histological methods have the highest specificity for TB in terms of diagnosing active TB and the number of TB patients in whom extrapulmonary TIGRAs have been evaluated is still limited, a comparison of local and systemic MTB-specific immune responses is a promising technique to rapidly distinguish active TB from latent MTB infection in routine clinical practice.

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Update in Tuberculosis 2006

Cited by 16 publications

References 51 publications

Identification of Mycobacterium tuberculosis-Specific Th1, Th17 and Th22 Cells Using the Expression of CD40L in Tuberculous Pleurisy

Identification of Mycobacterium tuberculosis-Specific Th1, Th17 and Th22 Cells Using the Expression of CD40L in Tuberculous Pleurisy

7. Immunologic lung disease

Suttons’s Law: Local Immunodiagnosis of Tuberculosis

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