Upcycling umbilical cords: bridging regenerative medicine with neonatology

Moreira, Alvaro; Alayli, Yasmeen; Balgi, Saloni; Winter, Caitlyn; Kahlenberg, Samuel; Mustafa, Shamimunisa B.; Hornsby, Peter J.

doi:10.1080/14767058.2017.1405387

Cited by 12 publications

(5 citation statements)

References 73 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The perinatal period offers an opportune time to collect tissues, in a non-invasive approach, that are rich in hematopoietic and mesenchymal stem cells [Weiss, and Troyer, 2006; Messerli et al, 2013; Yousefifard et al, 2016; Moreira et al, 2017]. In this study, we sought to evaluate characteristics in umbilical cord-derived cells isolated from preterm and term infants.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Preterm and Term Wharton’s Jelly-Derived Mesenchymal Stem Cell Properties in Different Oxygen Tensions

Balgi-Agarwal¹,

Winter²,

Corral³

et al. 2018

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have shown promise as therapeutic agents in treating morbidities associated with premature birth. MSCs derived from the human umbilical cord are easy to isolate and have low immunogenicity and a robust ability to secrete paracrine factors. To date, there are no studies evaluating preterm versus term umbilical cord tissue-derived MSCs. Therefore, our aim was twofold: (1) to compare stem cell properties in preterm versus term MSCs and (2) to examine the impact of oxygen tension on stem cell behavior. Umbilical cord tissue was obtained from 5 preterm and 5 term neonates. The cells were isolated and characterized as MSCs in accordance with the International Society for Cellular Therapy. We exposed MSCs to different oxygen tensions to examine the impact of environmental factors on cell performance. We studied the following stem cell properties: (i) motility, (ii) proliferation, (iii) senescence, (iv) cell viability, (v) colony-forming unit efficiency, and (vi) inflammatory cytokine expression. Under normoxia (21% O₂), cells from preterm and term infants had similar properties. Under hypoxic conditions (1% O₂), term MSCs had better cell proliferation; however, cells exposed to hyperoxia (90% O₂) had the slowest motility and lowest cell viability (p < 0.05). There was no difference in the expression of senescence or cytokine expression between the groups. The term cells demonstrated more colony-forming efficiency than the preterm cells. In sum, our preliminary findings suggest that MSCs derived from term and preterm umbilical cords have similar characteristics, offering the potential of future autologous/allogeneic MSC transplants in neonates.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Preterm and Term Wharton’s Jelly-Derived Mesenchymal Stem Cell Properties in Different Oxygen Tensions

Balgi-Agarwal¹,

Winter²,

Corral³

et al. 2018

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

show abstract

“…To characterize the cells as MSCs, we subjected the cells to flow cytometric analysis and in vitro differentiation as we have previously described 31,32 . The cells were positive for CD90, CD73, and CD105 and negative for CD11b, as expected for MSCs 33 .…”

Section: Isolation and Growth Of Human Mscsmentioning

confidence: 99%

The Route by Which Intranasally Delivered Stem Cells Enter the Central Nervous System

et al. 2018

Self Cite

View full text Add to dashboard Cite

Intranasal administration is a promising route of delivery of stem cells to the central nervous system (CNS). Reports on this mode of stem cell delivery have not yet focused on the route across the cribriform plate by which cells move from the nasal cavity into the CNS. In the current experiments, human mesenchymal stem cells (MSCs) were isolated from Wharton’s jelly of umbilical cords and were labeled with extremely bright quantum dots (QDs) in order to track the cells efficiently. At 2 h after intranasal delivery in immunodeficient mice, the labeled cells were found under the olfactory epithelium, crossing the cribriform plate adjacent to the fila olfactoria, and associated with the meninges of the olfactory bulb. At all times, the cells were separate from actual nerve tracts; this location is consistent with them being in the subarachnoid space (SAS) and its extensions through the cribriform plate into the nasal mucosa. In their location under the olfactory epithelium, they appear to be within an expansion of a potential space adjacent to the turbinate bone periosteum. Therefore, intranasally administered stem cells appear to cross the olfactory epithelium, enter a space adjacent to the periosteum of the turbinate bones, and then enter the SAS via its extensions adjacent to the fila olfactoria as they cross the cribriform plate. These observations should enhance understanding of the mode by which stem cells can reach the CNS from the nasal cavity and may guide future experiments on making intranasal delivery of stem cells efficient and reproducible.

show abstract

“…23 Unlike embryonic stem cells, human Umbilical Cord MSCs (hUC-MSCs) transplantation has not been shown to increase the risk of teratoma formation. 36 In addition, compared to other MSC sources, UC-MSCs are recognized as a premier source of stem cells for transplantation. 31 hUC-MSCs act as a potential cell-based treatment for degenerative diseases.…”

Section: Umbilical Cord Mscsmentioning

confidence: 99%

“…UC‐MSCs are a little positive for embryonic stem cell markers (Oct4, NANOG, SOX2, and KLF4), indicating the more preliminary of these cells than adult stem cells 23 . Unlike embryonic stem cells, human Umbilical Cord MSCs (hUC‐MSCs) transplantation has not been shown to increase the risk of teratoma formation 36 . In addition, compared to other MSC sources, UC‐MSCs are recognized as a premier source of stem cells for transplantation 31 .…”

Section: Resources For Mscs Isolationmentioning

confidence: 99%

Application of mesenchymal stem cells in regenerative medicine: A new approach in modern medical science

Ebrahimi,

Pirouzmand,

Cosme Pecho

et al. 2023

Biotechnology Progress

View full text Add to dashboard Cite

Mesenchymal Stem Cells (MSCs) are non‐hematopoietic and multipotent stem cells, which have been considered in regenerative medicine. These cells are easily separated from different sources, such as bone marrow (BM), umbilical cord (UC), adipose tissue (AT), and etc. MSCs have the differentiation capability into chondrocytes, osteocytes, and adipocytes; This differentiation potential along with the paracrine properties have made them a key choice for tissue repair. MSCs also have various advantages over other stem cells, which is why they have been extensively studied in recent years. The effectiveness of MSCs‐based therapies depend on several factors, including differentiation status at the time of use, concentration per injection, delivery method, the used vehicle, and the nature and extent of the damage. Although, MSCs have emerged promising sources for regenerative medicine, there are potential risks regarding their safety in their clinical use, including tumorigenesis, lack of availability, aging, and sensitivity to toxic environments. In this study, we aimed to discuss how MSCs may be useful in treating defects and diseases. To this aim, we will review recent advances of MSCs action mechanisms in regenerative medicine, as well as the most recent clinical trials. We will also have a brief overview of MSCs resources, differences between their sources, culture conditions, extraction methods, and clinical application of MSCs in various fields of regenerative medicine.

show abstract

Upcycling umbilical cords: bridging regenerative medicine with neonatology

Cited by 12 publications

References 73 publications

Comparison of Preterm and Term Wharton’s Jelly-Derived Mesenchymal Stem Cell Properties in Different Oxygen Tensions

Comparison of Preterm and Term Wharton’s Jelly-Derived Mesenchymal Stem Cell Properties in Different Oxygen Tensions

The Route by Which Intranasally Delivered Stem Cells Enter the Central Nervous System

Application of mesenchymal stem cells in regenerative medicine: A new approach in modern medical science

Contact Info

Product

Resources

About