Up-regulation of VEGF, c-fms and COX-2 expression correlates with severity of cervical cancer precursor (CIN) lesions and invasive disease

Hammes, Luciano Serpa; Tekmal, Rajeshwar Rao; Naud, Paulo; Edelweiss, Maria Isabel Albano; Kirma, Nameer B.; Valente, Philip T.; Syrjänen, Karí; Cunha-Filho, João Sabino

doi:10.1016/j.ygyno.2008.04.038

Cited by 52 publications

(35 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, which is a continuation of our earlier investigation, a higher level of M-CSF was also observed [25]. Furthermore, the key role of this factor and its receptor in the initiation of carcinogenesis and tumor growth stimulation has been suggested in previous publications by other authors [9,10].…”

Section: Discussionsupporting

confidence: 90%

“…Interestingly, in our study M-CSF levels in the cancer group were significantly higher compared to CIN patients. Our results confirm the findings of other researchers [9,32]. On the other hand, this data contradicts that of Punnonen et al [23] who postulated that malignant and benign tumors generally induce similar levels of cytokine production.…”

Section: Discussionsupporting

confidence: 84%

“…www.fhc.viamedica.pl M-CSF, its receptor (M-CSFR) and c-fms (protooncogene encodes a transmembrane glycoprotein which is identical to the receptor for M-CSF) overexpressions were confirmed on the lines of cervical cancer in comparison with the patients with benign lesions (CIN) and to the control group. The blockade of M-CSFR resulted in the inhibition of tumor growth and the intensification of the apoptosis processes in cancer cells [9,10]. The production of G-CSF and GM-CSF has been demonstrated in a number of nonhematologic malignancies including tumors of the cervix [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients

Ławicki

Będkowska

Gacuta-Szumarska

et al. 2012

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:In this study, we compared plasma levels and the diagnostic utility of hematopoietic growth factors (HGFs) with SCC-Ag in cervical cancer patients in relation to control groups and cervical intraepithelial neoplasia (CIN) patients and healthy subjects. Pretreatment plasma levels of HGFs (SCF, GM-CSF, G-CSF and M-CSF) were determined by the use of immunoenzyme assay (ELISA), and SCC-Ag by chemiluminescent microparticle immunoassay (CMIA). Significantly different concentrations of GM-CSF, G-CSF and M-CSF were observed in the group of patients with cervical cancer and CIN compared to the healthy controls. Significant differences in plasma levels of GM-CSF and M-CSF between cervical cancer and benign lesions patients were also found. The HGFs and SCC-Ag diagnostic specificities received high values. The diagnostic sensitivity and the predictive value of a positive and negative test result were higher for M-CSF than for antigen SCC in the cancer group. The M-CSF area under the ROC curve (AUC) was the largest from hematopoietic cytokines and SCC-Ag. These results suggest the potential utility of M-CSF as a good candidate for a marker of cervical cancer as well as benign lesions of this organ (CIN).

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients

Ławicki

Będkowska

Gacuta-Szumarska

et al. 2012

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…The staining was evaluated as previously described (24). The B7-H3 immunostaining densities were assessed according to the H-score method described by Hammes et al (25). Assessment of the infiltration densities of CD8 + T cells and CD68 + macrophages was performed in both the tumor stroma and tumor nest.…”

Section: Methodsmentioning

confidence: 99%

Cancer cell‑expressed B7‑H3 regulates the differentiation of tumor‑associated macrophages in human colorectal carcinoma

et al. 2017

View full text Add to dashboard Cite

Abstract. Co-stimulatory molecule B7 homolog 3 protein (B7-H3) has been described as an important tumor antigen in various human tumors. The exact role of B7-H3 in tumor progression and its receptor are still ambiguous. The phenotype and the function of tumor-associated macrophages (TAMs) in human solid tumors are complicated and could contribute to the shaping of the tumor microenvironment. In the present study, B7-H3 expression and lymphocyte infiltration were investigated by immunohistochemistry in 117 colorectal carcinoma (CRC) patients. B7-H3 expression was positively associated with the infiltrating density of macrophage in CRC tissues, and B7-H3 expression and the infiltrating density of macrophages were negatively associated with the overall survival rate of patients. The putative B7-H3 receptor was found on activated monocytes and macrophages, indicating the direct function of B7-H3 signal on macrophages. Additional results revealed that during the differentiation of TAMs, B7-H3 promoted the polarization of type 2 macrophages (M2s) and switch of the M1 phenotype to the M2 phenotype. Thus, B7-H3 signaling promotes M2 differentiation via the putative receptor on monocytes and macrophages. Targeting the manipulation of TAMs through the B7-H3 pathway may be valuable for the development of novel immunotherapeutic strategies against human CRC.

show abstract

“…VEGF, by binding with its receptor, acts on vascular endothelial cells and tumor cells in paracrine and autocrine manners, and then influences the proliferation of vascular endothelial cells, basal membrane hydrolysis, the formation of blood vessels, and the biological behavior of tumor cells (Bremnes et al, 2006). The synthesis and secretion of VEGF (Hammes et al, 2008;Samouelian et al, 2008) increases in the normal cervical epithelium, inducing cervical epithelium damage, gradually increasing the number of cancerous cells. In addition, its angiogenic capacity increases significantly along with the elevated expression of VEGF.…”

Section: Discussionmentioning

confidence: 99%

Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer

Liu

Wei

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. This study aimed to investigate the inhibition of vascular endothelial growth factor (VEGF) by small hairpin RNA (shRNA) interference combined with radiotherapy on the growth of cervical cancer SiHa cell xenografts in nude mice. The effective pVEGFshRNA plasmid was screened by reverse transcription-polymerase chain reaction (RT-PCR), and the cell apoptosis rate was estimated by flow cytometry. A nude mouse cervical xenograft model was established and all models were divided into four groups: blank control, VEGF shRNA, radiotherapy, and combined treatment. We calculated the tumor growth curve and the inhibitory rate. The histopathological changes of the microvascular density and VEGF protein expression were observed by immunohistochemistry. The expressions of VEGF and hypoxia inducible factor-1α (HIF-1α) proteins in the tumor tissue were analyzed via Western blot. VEGF shRNA interference inhibited the expression of radiation-induced VEGF (P < 0.05), induced apoptosis (P < 0.05), downregulated the HIF-1α protein, and reduced angiogenesis. Compared with the other three groups, the combined treatment group showed the most significant effects (P < 0.01). VEGF shRNA interference combined with radiotherapy promotes the radiosensitivity of tumors via improvement of the hypoxic microenvironment.

show abstract

Up-regulation of VEGF, c-fms and COX-2 expression correlates with severity of cervical cancer precursor (CIN) lesions and invasive disease

Cited by 52 publications

References 39 publications

Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients

Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients

Cancer cell‑expressed B7‑H3 regulates the differentiation of tumor‑associated macrophages in human colorectal carcinoma

Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer

Contact Info

Product

Resources

About