Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients

Abstract

Abstract:In this study, we compared plasma levels and the diagnostic utility of hematopoietic growth factors (HGFs) with SCC-Ag in cervical cancer patients in relation to control groups and cervical intraepithelial neoplasia (CIN) patients and healthy subjects. Pretreatment plasma levels of HGFs (SCF, GM-CSF, G-CSF and M-CSF) were determined by the use of immunoenzyme assay (ELISA), and SCC-Ag by chemiluminescent microparticle immunoassay (CMIA). Significantly different concentrations of GM-CSF, G-CSF and M-CSF were observed in the group of patients with cervical cancer and CIN compared to the healthy controls. Significant differences in plasma levels of GM-CSF and M-CSF between cervical cancer and benign lesions patients were also found. The HGFs and SCC-Ag diagnostic specificities received high values. The diagnostic sensitivity and the predictive value of a positive and negative test result were higher for M-CSF than for antigen SCC in the cancer group. The M-CSF area under the ROC curve (AUC) was the largest from hematopoietic cytokines and SCC-Ag. These results suggest the potential utility of M-CSF as a good candidate for a marker of cervical cancer as well as benign lesions of this organ (CIN).

Diagnostic Power of Cytokine M-CSF, Metalloproteinase 2 (MMP-2) and Tissue Inhibitor-2 (TIMP-2) in Cervical Cancer Patients Based on ROC Analysis

“…Only the plasma levels of TIMP-2 were statistically significantly lower when compared to healthy controls. These findings are in agreement with our previous study showing that the median levels of M-CSF in cervical cancer patients are higher (510.55 pg/mL) than in healthy subjects (290.82 pg/mL) [32]. Our observation is supported by data regarding M-CSF from a previous report by Ławicki et al [33] in breast cancer patients.…”

The plasma concentration of VEGF, HE4 and CA125 as a new biomarkers panel in different stages and sub-types of epithelial ovarian tumors

“…Results of this study suggest that combining VEGF with CA125 and HE4 measurements might enable improved early detection of this cancer as compared with use of either marker alone or of both comparative tumor markers. This observation is in accordance to our previous papers, in which we evaluated diagnostic criteria of selected cytokines and commonly used tumor markers in various gynecological malignancies [51,57,58]. Several studies have found sensitivity to be greater than in either marker alone: VEGF and CEA (carcinoembrionic antigen) in colorectal [59] or HE4 and CA125 in ovarian cancer patients [5,45].…”

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

“…Several studies have confirmed this observation - they found sensitivity to be greater than in either marker used alone: MMP-7, CCL18 (CC chemokine 18), CCL11 (CC chemokine 11) and CA125 in ovarian cancer (SE in the early stages 94.4%) [46]. This conclusion is also in accordance with our previous papers in which the diagnostic criteria of selected cytokines and aforementioned tumor markers were evaluated in various gynecological malignancies [47, 48]. Diagnostic specificity (SP) reached very high and equal values for all biomarkers studied and this was in accordance [40, 43] with the available literature in the course of various malignant and benign diseases.…”