Up-Regulation of MiR-452 Inhibits Metastasis of Non-Small Cell Lung Cancer by Regulating BMI1

Abstract

Background/Aims: MicroRNAs (miRNAs) have been regarded as a new class of regulators in cellular processes in non-small cell lung cancer (NSCLC). However, the relationship between miR-452 and the development of NSCLC remains unclear. Methods: qRT-PCR was used to detect the expression of miR-452 and its target gene in NSCLC samples (n=60). The transwell assay was used to test the cell invasion capability. The regulation mechanism was confirmed by luciferase reporter assay and western blot assay. Results: In the current study, a relatively lower miR-452 and higher BMI1 expression levels were confirmed to be associated with advanced tumor stage and more extent of lymph nodes metastasis. In vitro, down-regulated miR-452 could enhance cell invasion capability. Furthermore, miR-452 modulated BMI1 expression by binding to its 3ʹ-UTR. The enhancement of cell invasion capability induced by down-regulated miR-452 was eliminated by repression of BMI1. Conclusions: Our results suggest that miR-452 plays a vital role in development of NSCLC, and this miR-452-BMI1 pathway might generate a novel insight into the treatment of NSCLC.

MicroRNA-452 suppresses pancreatic cancer migration and invasion by directly targeting B-cell-specific Moloney murine leukemia virus insertion site 1

“…miR-452 has been studied in numerous types of human cancer (15,16,25). For example, He et al (15) reported that the expression levels of miR-452 were decreased in non-small-cell lung cancer, and low expression levels of miR-452 were associated with advanced tumor stage and more extent of lymph nodes metastasis.…”

“…For example, He et al (15) reported that the expression levels of miR-452 were decreased in non-small-cell lung cancer, and low expression levels of miR-452 were associated with advanced tumor stage and more extent of lymph nodes metastasis. Functional studies have revealed that under-expression of miR-452 may induce cell invasion of non-small-cell lung cancer (15).…”

“…For example, He et al (15) reported that the expression levels of miR-452 were decreased in non-small-cell lung cancer, and low expression levels of miR-452 were associated with advanced tumor stage and more extent of lymph nodes metastasis. Functional studies have revealed that under-expression of miR-452 may induce cell invasion of non-small-cell lung cancer (15). In addition, miR-452 was revealed to negatively regulate BMI1 expression by binding to its 3'UTR, and the enhanced cell invasion induced by downregulated miR-452 may be eliminated by knockdown of BMI1 (15).…”

“…Functional studies have revealed that under-expression of miR-452 may induce cell invasion of non-small-cell lung cancer (15). In addition, miR-452 was revealed to negatively regulate BMI1 expression by binding to its 3'UTR, and the enhanced cell invasion induced by downregulated miR-452 may be eliminated by knockdown of BMI1 (15). In breast cancer, miR-452 was downregulated in adriamycin-resistant MCF-7 cells compared with in parental MCF-7 cells.…”

“…miR-452 has been studied in several types of cancer, including non-small cell lung cancer (15), breast cancer (16), bladder cancer (17) and hepatocellular carcinoma (18). However, the expression, biological function and molecular mechanism of miR-452 in pancreatic cancer remains to be fully elucidated.…”