Up-regulation of miR-326 regulates pro-inflammatory cytokines targeting TLR-4 in buffalo granulosa cells

Chaurasiya, Vaishali; Kumari, Sneha; Onteru, Suneel Kumar; Singh, Dheer

doi:10.1016/j.molimm.2020.01.019

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…PRRs comprise TLRs, RIG-I-like receptors (RLRs), and NOD-like receptors (NLRs). TLRs activate viral responses by recognizing pathogenic microorganisms such as viruses, bacteria, and mycoplasmas to release inflammatory cytokines [ 10 ]. TLRs are important constituents of the innate immune system, which were applied to recognize and defend against foreign pathogens.…”

Section: Introductionmentioning

confidence: 99%

“…TLRs are conserved and approximately comprised 10 functional TLRs (TLR1 to TLR10) in humans and 12 (TLR1 to TLR9, TLR11 to TLR13) in mice. TLR1, TLR2, TLR4, TLR5, TLR6, and TLR10 are expressed on the human cell surface, while TLR3, TLR7, TLR8, and TLR9 are expressed on the membrane of intracellular vesicles [ 9 , 10 ]. TLR9 is a member of the TLR family whose function is triggered by the unmethylated CpG motif of microbial DNA, which initiates the immune response by inducing cytokines and many molecules [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

Liu

Zhou

et al. 2022

Computational and Mathematical Methods in Medicine

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Swine pneumonia commonly known as swine pasteurellosis is an infectious disease of swine caused by Pasteurella multocida infection. It has been reported that Toll-like receptors (TLRs) play a vital role in swine pneumonia progression. However, the underlying mechanism has not been elucidated. This research was aimed at investigating the molecular mechanism by which TLR9 regulates swine pneumonia progression. Our findings illustrated that the HD-13 strain of Pasteurella multocida D (HD-13) accelerated TLR9 expression in porcine alveolar macrophage 3D4/21 cells; HD-13 activated the inflammatory response via accelerating TLR9 expression. Mechanistically, HD-13 activated mitogen-activated protein kinase (MAPK) and nuclear factor kB (NF-κB) signals. In conclusion, HD-13 may activate MAPK and NF-κB pathways via accelerating TLR9 expression, thereby accelerating the inflammatory response in the progression of swine pneumonia. TLR9 may serve as a novel therapeutic target for swine pneumonia. Our research may provide a theoretical basis for the prevention and treatment of swine pneumonia.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

Liu

Zhou

et al. 2022

Computational and Mathematical Methods in Medicine

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“…It has been reported that a variety of miRNAs contained in exosomes can regulate the expression of microglia Toll-like receptors (TLRs), thus acting on NF-κB to regulate microglia polarization ( Hajinejad and Sahab-Negah, 2021 ). miR-223, miR-101-3p, miR-26a -5p, miR-326, miR-182, miR-17-5p, miR-140-5p, miR-9, miR-let7, and miR-181c play a role in inhibiting M1 microglia polarization by downregulating TLR expression including TLR2 and TLR4 ( Kumar et al, 2015 ; Wang et al, 2015 ; Li et al, 2016 ; Li et al, 2017 ; Qian et al, 2017 ; Li et al, 2019 ; Li et al, 2020a ; Yang et al, 2020a ; Chaurasiya et al, 2020 ; Huang et al, 2020 ). In addition, miR-26b-5p inhibited the TLR signaling pathway by regulating the reduction of CH25H protein expression, which in turn inhibited microglia M1 polarization ( Li et al, 2020b ).…”

Section: Exosome and Microgliamentioning

confidence: 99%

Microglia Polarization: A Novel Target of Exosome for Stroke Treatment

Wan

Zhao

Gao

et al. 2022

Front. Cell Dev. Biol.

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The vast majority of cells in the human body are capable of secreting exosomes. Exosomes have become an important vehicle for signaling between cells. Exosomes secreted by different cells have some of the structural and functional properties of that cell and thus have different regulatory functions. A large number of recent experimental studies have shown that exosomes from different sources have different regulatory effects on stroke, and the mechanisms still need to be elucidated. Microglia are core members of central intrinsic immune regulatory cells, which play an important regulatory role in the pathogenesis and progression of stroke. M1 microglia cause neuroinflammation and induce neurotoxic effects, while M2 microglia inhibit neuroinflammation and promote neurogenesis, thus exerting a series of neuroprotective effects. It was found that there is a close link between exosomes and microglia polarization, and that exosome inclusions such as microRNAs play a regulatory role in the M1/M2 polarization of microglia. This research reviews the role of exosomes in the regulation of microglia polarization and reveals their potential value in stroke treatment.

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“…In response to DNA damage, Chk1 and Chk2 are activated by PI3 kinase-related kinases ATM and ATR, respectively, aiming at many downstream substrates that coordinate cell cycle checkpoint activation, DNA restitution, and apoptosis [11]. Moreover, Chk1/2 has also been implicated in anaphase entry, chromosome condensation, and maintenance of genome integrity in somatic cells in the absence of DNA damage [12].…”

Section: Introductionmentioning

confidence: 99%

Chk1/2 inhibitor AZD7762 blocks the growth of preantral follicles by inducing apoptosis, suppressing proliferation, and interfering with the cell cycle in granulosa cells

Liu

Chen

Zhang

et al. 2022

Preprint

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Background Checkpoint kinases 1/2 (Chk1/2) has an important role in somatic cells development and oocyte meiotic maturation. However, the role of Chk1/2 in folliculogenesis has not been fully elucidated. The aim of this study was to assess the effects of Chk1/2 inhibition on ovarian folliculogenesis and granulosa cells development in mice. Methods Preantral follicles (100 µm- 120 µm) and granulosa cells from pre-ovulatory follicles (pre-GCs) of mice were isolated and cultured with or without Chk1/2 inhibitor AZD7762. Preantral follicles were cultured for 96 h. Then, follicle morphology and follicular growth were assessed every 48 h. Granulosa cells were cultured for 48 h with or without AZD7762, after which cell apoptosis, cell proliferation, and cell cycle analysis were assessed; meanwhile, the mRNA expression of PCNA and Bax were measured by real-time RT-PCR, and PCNA and Bax protein were measured by Western blot. Results Compared with control follicles, AZD7762 inhibited growth of preantral follicles. Furthermore, inhibition of Chk1/2 significantly induced apoptosis and inhibited the proliferation of granulosa cells, arrested cell cycle at S and G2/M phases, and decreased G1 phase fraction. Also, the expression of PCNA mRNA and protein were significantly reduced, while Bax mRNA and protein were significantly increased post AZD7762 treatment in granulosa cells. Conclusions This study revealed that Chk1 and Chk2 have a crucial role during preantral follicular development by regulating the proliferation and apoptosis of granulosa cells.

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Up-regulation of miR-326 regulates pro-inflammatory cytokines targeting TLR-4 in buffalo granulosa cells

Cited by 8 publications

References 37 publications

HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

Microglia Polarization: A Novel Target of Exosome for Stroke Treatment

Chk1/2 inhibitor AZD7762 blocks the growth of preantral follicles by inducing apoptosis, suppressing proliferation, and interfering with the cell cycle in granulosa cells

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