Up-regulation of lncRNA NEAT1 in cerebral ischemic stroke promotes activation of astrocytes by modulation of miR-488-3p/RAC1

Zheng, Haijian; Gai, Zheng; Liu, Guanglan; Wang, Ling

doi:10.1007/s00221-022-06519-z

Cited by 8 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 58 Hsa-miR-488-3p is also involved in regulating the activation of astrocytes in IS. 59 In this study, we also found that hsa-miR-3150b-3p, hsa-miR-541-5p, hsa-miR-184 and hsa-miR-488-3p were abnormally expressed in IS. Therefore, we speculate that hsa-miR-3150b-3p, hsa-miR-541-5p, hsa-miR-184 and hsa-miR-488-3p may target the regulation of RRAS2, PPM1B, CLDN7 and ADCY3 in the progression of IS.…”

Section: Discussionsupporting

confidence: 64%

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Zhang¹,

Han²,

Xu³

et al. 2023

IJGM

View full text Add to dashboard Cite

Background:The specific molecular mechanistic link between atherosclerotic plaques and ischemic stroke (IS) is not clear. The aim of this study is to explore the potential molecular relationship between atherosclerotic plaques and IS. Methods: All data were downloaded from the Gene Expression Omnibus (GEO) database. Key hub differentially expressed mRNAs (DEmRNAs) related to atherosclerotic plaques and IS were identified by differential expression analysis and least absolute shrinkage and selection operator (LASSO) analysis. Subsequently, a diagnostic model was established based on the expression of key hub DEmRNAs and logistic regression. In order to understand the molecular mechanism of key hub DEmRNAs, the transcription factor (TF) regulatory network and mRNA-miRNA-lncRNA regulatory network were also constructed. In addition, functional enrichment analysis and single-sample Gene Set Enrichment Analysis (ssGSEA) analysis were also performed. Results: Four key hub DEmRNAs (ADCY3, CLDN7, PPM1B and RRAS2) were identified by differential expression analysis and LASSO analysis. Moreover, the diagnostic model based on four key hub DEmRNAs has excellent diagnostic accuracy. We also found that Type 1 T helper cell may be associated with IS caused by atherosclerosis based on ssGSEA analysis. In the mRNA-miRNA-lncRNA regulatory network, we found that multiple signaling axes such as RRAS2-hsa-miR-3150b-3p-ILF3-AS1, PPM1B-hsa-miR -541-5p-LINC00294, CLDN7-hsa-miR-184-LINC00467 and ADCY3-hsa-miR-488-3p-URB1-AS1 may play an important role in the progression of IS. In addition, some signaling pathways, including chemokine signaling pathway, MAPK signaling pathway and cAMP signaling pathway, may be involved in regulating IS. Conclusion:The identified key molecules, signaling pathways and immune cells may help to provide a theoretical basis for exploring the relationship between atherosclerotic plaque and the progression of IS.

show abstract

Section: Discussionsupporting

confidence: 64%

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Zhang¹,

Han²,

Xu³

et al. 2023

IJGM

View full text Add to dashboard Cite

show abstract

“…Another noteworthy example of a PhenomeX substance use-linked candidate gene with altered expression in all three major glial prototypes, including astrocytes, microglia and ODC/OPC (Fig. 5 ) is the nuclear paraspeckles-associated long non-coding RNA, NEAT1 , which has been broadly linked to astrocytic and microglial activation 50 , 51 , and has been previous reported as up-regulated in ventral striatum of heroin abusers 52 . Consistent with these findings, multiple inflammatory response- and cell activation- and migration-associated GOs involving NEAT1 , and additional PhenomeX substance use associated glial DEGs such as ATP1B3 , BARD1 and HESX1 were significantly enriched among the glial DEGs of the present study (Fig.…”

Section: Resultsmentioning

confidence: 98%

Single nucleus transcriptomics of ventral midbrain identifies glial activation associated with chronic opioid use disorder

Wei,

Lambert,

Valada

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Dynamic interactions of neurons and glia in the ventral midbrain mediate reward and addiction behavior. We studied gene expression in 212,713 ventral midbrain single nuclei from 95 individuals with history of opioid misuse, and individuals without drug exposure. Chronic exposure to opioids was not associated with change in proportions of glial and neuronal subtypes, however glial transcriptomes were broadly altered, involving 9.5 − 6.2% of expressed genes within microglia, oligodendrocytes, and astrocytes. Genes associated with activation of the immune response including interferon, NFkB signaling, and cell motility pathways were upregulated, contrasting with down-regulated expression of synaptic signaling and plasticity genes in ventral midbrain non-dopaminergic neurons. Ventral midbrain transcriptomic reprogramming in the context of chronic opioid exposure included 325 genes that previous genome-wide studies had linked to risk of substance use traits in the broader population, thereby pointing to heritable risk architectures in the genomic organization of the brain’s reward circuitry.

show abstract

“…9h). 122,123 We identified regulatory networks involving 12 lncRNAs, 16 miRNAs, and 6 mRNAs that play critical roles in PD. By elucidating the regulatory interactions within this network, researchers can uncover novel pathways and processes involved in disease development and progression.…”

Section: Discussionmentioning

confidence: 99%

Elucidating the Role of a Shared lncRNA-miRNA-mRNA Network in Exacerbating Parkinson’s Disease Symptoms in the Context of COVID-19 Infection

Yousefi,

Hashemi,

Peymani

et al. 2024

Gene Expr

View full text Add to dashboard Cite

Background and objectives: Parkinson's disease (PD) is a common neurodegenerative disorder with unclear molecular mechanisms. Noncoding RNAs, such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), have been identified as critical regulators of gene expression. This study aimed to investigate the triple network of lncRNA-miRNA-mRNA, known as competing endogenous RNAs (ceRNAs), and to identify essential lncRNAs that regulate PD-related gene expression through their target miRNAs. The study also identified a common triple network between COVID-19 and PD that may contribute to exacerbating PD symptoms. Methods:A bioinformatics approach was employed to construct a PD ceRNA network using common PD genes, miRNAs and lncRNAs with the highest interaction with their targets. Also, a PD-COVID-19 triple network was constructed by integrating PD network nodes into the COVID-19 network. Results:The PD ceRNA network comprised 34 nodes, including 12 lncRNAs, 16 miRNAs with interconnections and six mRNAs, some of which were related to COVID-19. The network showed parallel expression of the SNCA and PARK7 genes as well as the NEAT1 and MALAT1 lncRNAs in both PD and COVID-19. Conclusions:This study provide insights into the molecular mechanisms underlying the worsening of symptoms in PD patients with COVID-19. The PD and COVID-19 ceRNA network indicates that coronavirus could worsen PD symptoms by altering the expression of some genes related to PD. Therefore, COVID-19 could dysregulate the common RNAs involved in PD through lncRNAs, miRNAs.

show abstract

Up-regulation of lncRNA NEAT1 in cerebral ischemic stroke promotes activation of astrocytes by modulation of miR-488-3p/RAC1

Cited by 8 publications

References 25 publications

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Single nucleus transcriptomics of ventral midbrain identifies glial activation associated with chronic opioid use disorder

Elucidating the Role of a Shared lncRNA-miRNA-mRNA Network in Exacerbating Parkinson’s Disease Symptoms in the Context of COVID-19 Infection

Contact Info

Product

Resources

About