Up‐regulated long non‐coding RNA H19 contributes to proliferation of gastric cancer cells

Yang, Feng; Bi, Jiacheng; Xue, Xindong; Zheng, Luming; Zhi, Kangkang; Hua, Jinlian; Fang, Guoen

doi:10.1111/j.1742-4658.2012.08694.x

Cited by 405 publications

(315 citation statements)

References 28 publications

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“…18 Increased expression of H19 has been observed in some cancers, such as gastric 25,30 and bladder cancer, 16 and decreased expression in others, such as HCC, 13 suggesting that H19 involves either oncogenic or tumor suppressor properties. Hao et al 10 introduced a construct expressing the H19 gene into the kidney tumor cells and observed that overexpression of H19 induced a slower growth rate, a much lower anchorage-independent growth rate in soft agar, and no tumor formation in mice, which indicate the tumor suppressor properties of H19 in kidney tumors.…”

Section: Discussionmentioning

confidence: 99%

“…Increased expression of H19 has been observed in some cancers, such as gastric 25,30 and bladder cancer, 16 and decreased expression in others, such as hepatocellular carcinoma (HCC), 13 suggesting that H19 may have either oncogenic or tumor suppressor properties, although the exact mechanism is still elusive. 11 A previous study showed that c-Myc (Myc) significantly induces the expression of H19 in diverse cell types, including breast epithelial, glioblastoma, and fibroblast cells, suggesting that H19 may play an important role in glioblastoma development.…”

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confidence: 99%

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Increased level of H19 long noncoding RNA promotes invasion, angiogenesis, and stemness of glioblastoma cells

Jiang

Yan

et al. 2016

JNS

155

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M alignant gliomas are the most common type of primary malignant brain tumor, and more than half of all gliomas are glioblastomas (Grade IV astrocytoma), one of the most aggressive and lethal types of brain tumor. Glioblastoma cells easily infiltrate into the normal cerebral cortex, ultimately resulting in the death of the patient. Well-defined risk factors for glioblastoma include radiation exposure and certain genetic syndromes. 21Several molecular and genomic datasets have recently been generated that have allowed identification of at least 4 subtypes of glioblastoma: classical, mesenchymal, proneural, and neural.14 Previous studies in glioblastoma geabbreviatioNs FBS = fetal bovine serum; HCC = hepatocellular carcinoma; HUVEC = human umbilical vein endothelial cell; lncRNA = long noncoding RNA; ncRNA = noncoding RNA; RT-qPCR = real-time quantitative reverse transcription polymerase chain reaction; SD = standard deviation. 3 Department of Respiratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing; and 4 Department of Oncology, The Second Affiliated Hospital of Suzhou University, Suzhou, China obJective Increased levels of H19 long noncoding RNA (lncRNA) have been observed in many cancers, suggesting that overexpression of H19 may be important in the development of carcinogenesis. However, the role of H19 in human glioblastoma is still unclear. The object of this study was to examine the level of H19 in glioblastoma samples and investigate the role of H19 in glioblastoma carcinogenesis. methods Glioblastoma and nontumor brain tissue specimens were obtained from tissue obtained during tumor resection in 30 patients with glioblastoma. The level of H19 lncRNA was detected by real-time quantitative reverse transcription polymerase chain reaction. The role of H19 in invasion, angiogenesis, and stemness of glioblastoma cells was then investigated using commercially produced cell lines (U87 and U373). The effects of H19 overexpression on glioblastoma cell invasion and angiogenesis were detected by in vitro Matrigel invasion and endothelial tube formation assay. The effects of H19 on glioblastoma cell stemness and tumorigenicity were investigated by neurosphere formation and an in vivo murine xenograft model. results The authors found that H19 is significantly overexpressed in glioblastoma tissues, and the level of expression was associated with patient survival. In the subsequent investigations, the authors found that overexpression of H19 promotes glioblastoma cell invasion and angiogenesis in vitro. Interestingly, H19 was also significantly overexpressed in CD133 + glioblastoma cells, and overexpression of H19 was associated with increased neurosphere formation of glioblastoma cells. Finally, stable overexpression of H19 was associated with increased tumor growth in the murine xenograft model. coNclusioNs The results of this study suggest that increased expression of H19 lncRNA promotes invasion, angiogenesis, stemness, and tumorigenicity of glioblastoma cells. Taken together, the...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Increased level of H19 long noncoding RNA promotes invasion, angiogenesis, and stemness of glioblastoma cells

Jiang

Yan

et al. 2016

JNS

155

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“…H19 is a member of a highly-conserved cluster of imprinted genes, and was the first lncRNA to be associated with cancer (12). Previous studies have indicated that H19 exhibits dysregulated expression in GC (13,14), hepatocellular carcinoma (15), bladder cancer (16), breast cancer (17) and esophageal cancer (18), and that itmay cause tumor promoting and suppressing effects (19). Niu et al (20) screened lncRNA expression profilesfrom colorectal cancer and adenoma samples, and identified a novel lncRNA, AK027294, which was associated with cell proliferation, migration and apoptosis in colorectal cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

et al. 2017

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Abstract. Long non-coding RNAs (lncRNAs) are emerging as a fundamental class of biological effect or molecules that perform pivotal functions in the regulation of the genome. With advances in bioinformatics and genomics, extensive identification and characterization of lncRNAs is now possible. They regulate cellular growth, differentiation and apoptosis. Dysregulation of lncRNAs has been associated with numerous types of human cancer. In the present study, the expression profile of differentially expressed genes (DEGs) and lncRNAs in gastric cancer (GC) samples and normal tissue samples was evaluated with bioinformatics. The biological functions of the predicted lncRNA TCONS_00068220 were focused on; the DEGs co-expressed with TCONS_00068220 were enriched in cancer-associated pathways. TCONS_00068220 was demonstrated to be upregulated in GC tissues and cell lines compared with normal controls. In addition, an increased rate of apoptosis was observed in NCI-N87 cells following transfection with small interfering RNA against TCONS_00068220. These data suggest that TCONS_00068220 may be associated with the pathogenesis of GC, and it may serve as a potential therapeutic target.

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“…Previously, lncRNA was thought to be 'junk nucleotides', but recent evidence indicates that lncRNA is as important in epigenetic modulation as miRNA (74). Aberrant expression of certain types of lncRNA has been associated with poorer prognosis in breast cancer (75), hepatocellular carcinoma (76) and lung carcinoma (77); and it has recently been demonstrated to possess clinical significance in GC (78)(79)(80)(81)(82)(83)(84)(85) (Table III). lncRNA in plasma is reportedly detectable in patients with GC, as with miRNA (86).…”

Section: Long Non-coding Rna (Lncrna)mentioning

confidence: 99%

Epigenetic modulation associated with carcinogenesis and prognosis of human gastric cancer

et al. 2017

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Abstract. Gastric cancer (GC) is a leading cause of cancer-related death, particularly in Asia. Epidemiological and other clinical studies have identified an association between a number of risk factors, including Helicobacter pylori, and GC. A number of studies have also examined genetic changes associated with the development and progression of GC. When considering the clinical significance of the expression of a specific gene, its epigenetic modulation should be considered. Epigenetic modulation appears to be a primary driver of changes in gastric tissue that promotes carcinogenesis and progression of GC and other neoplasms. The role of epigenetic modulation in GC carcinogenesis and progression has been widely studied in recent years. In the present review, recent results of epigenetic modulation associated with GC and their effects on clinical outcome are examined, with particular respect to DNA methylation, histone modulation and non-coding RNA. A number of studies indicate that epigenetic changes in the expression of specific genes critically affect their clinical significance and further study may reveal epigenetic changes as the basis for targeted molecular therapy or novel biomarkers that predict GC prognosis or extension of this often fatal disease.

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Up‐regulated long non‐coding RNA H19 contributes to proliferation of gastric cancer cells

Cited by 405 publications

References 28 publications

Increased level of H19 long noncoding RNA promotes invasion, angiogenesis, and stemness of glioblastoma cells

Increased level of H19 long noncoding RNA promotes invasion, angiogenesis, and stemness of glioblastoma cells

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

Epigenetic modulation associated with carcinogenesis and prognosis of human gastric cancer

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