Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

Abstract

Abstract. Long non-coding RNAs (lncRNAs) are emerging as a fundamental class of biological effect or molecules that perform pivotal functions in the regulation of the genome. With advances in bioinformatics and genomics, extensive identification and characterization of lncRNAs is now possible. They regulate cellular growth, differentiation and apoptosis. Dysregulation of lncRNAs has been associated with numerous types of human cancer. In the present study, the expression profile of differentially expressed genes (DEGs) and lncRNAs in gastric cancer (GC) samples and normal tissue samples was evaluated with bioinformatics. The biological functions of the predicted lncRNA TCONS_00068220 were focused on; the DEGs co-expressed with TCONS_00068220 were enriched in cancer-associated pathways. TCONS_00068220 was demonstrated to be upregulated in GC tissues and cell lines compared with normal controls. In addition, an increased rate of apoptosis was observed in NCI-N87 cells following transfection with small interfering RNA against TCONS_00068220. These data suggest that TCONS_00068220 may be associated with the pathogenesis of GC, and it may serve as a potential therapeutic target.

RETRACTED ARTICLE: LncRNA CASC11 promoted gastric cancer cell proliferation, migration and invasion in vitro by regulating cell cycle pathway

“…Long non-coding RNAs (lncRNAs) are a class of single-stranded RNA molecules which have no protein-coding capacity [7]. In the last few days, investigators discovered that LncRNA can participate in many important biological processes [8][9][10]. Zhang ZY et al unveiled that lncRNA CACS11 was upregulated in colorectal cancer tissues [11].…”

Long Noncoding RNA TCONS_00068220 Promotes Breast Cancer Progression by Regulating Epithelial-Mesenchymal Transition Marker E-Cadherin

“…Indeed, we observed that the proliferation of MCF-7 cells was significantly increased in response to enhanced expression of TCONS_00068220 while MDA-MB-231 cell proliferation was dramatically inhibited after TCONS_00068220 gene knockdown. This was strictly consistent with a previous study reporting that TCONS_00068220 suppresses cancer cell proliferation [ 16 ]. Moreover, migratory and invasive phenotypes were more apparent in cells with TCONS_00068220 overexpression.…”

“…Through microarray analysis, lncRNA TCONS_00068220 was previously identified to be differentially expressed in gastric cancer tissues and co-expressed with interleukin-7 [ 15 ], a putative tumor suppressor which can mitigate tumor growth and induce complete regression [ 28 ], indicating a significant role for TCONS_00068220 in tumor pathogenesis. In accordance with these findings, Zhao et al confirmed that TCONS_00068220 was upregulated in gastric cancer tissues and cell lines using qRT-PCR, and found that TCONS_00068220 gene silencing inhibited gastric cancer cell viability and induced cell apoptosis [ 16 ]. Together, these findings demonstrate that TCONS_00068220 acts as an oncogene in gastric carcinoma.…”

“…It is highly abundant in digestive organs and glands. Nevertheless, neither the effect of expression alterations of TCONS_00068220 nor its potential role, if any, in the pathogenesis of carcinomas has been reported, with the rare exceptions of previous studies suggesting that TCONS_00068220 was overexpressed in gastric cancer samples and that silencing its expression contributed to enhanced cell apoptosis [ 15 , 16 ].…”

Long Noncoding RNA (lncRNA) MIR22HG Suppresses Gastric Cancer Progression through Attenuating NOTCH2 Signaling

“…Furthermore, MALAT1 acts as an oncogene in gastric cancer, whose aberrantly up-regulation increases gastric cancer aggressiveness by regulating HMGB2 [16]. lncRNA TCONS_00068220 also suppresses gastric cancer cell apoptosis rate and it might be involved in the pathogenesis of gastric cancer [17]. These biological targets provide great possibilities for the early diagnosis and effective treatment strategy of gastric cancer.…”