Unveiling the potential of deep eutectic solvents to improve the conformational and colloidal stability of immunoglobulin G antibodies

Dhiman, Diksha; Marques, Ana Sofia C; Bisht, Meena; Tavares, Ana; Freire, Mara G.; Venkatesu, Pannuru

doi:10.1039/d2gc03415h

Cited by 11 publications

(4 citation statements)

References 60 publications

(90 reference statements)

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“…The samples were not filtered to study the insoluble/ irreversible aggregation of IgG Figure a distinctly enunciate that the native IgG in buffer solution has d H 252 nm with ζ-potential −0.80 mV at pH 7.0 at 25 °C which is consistent with the literature , (Table S7). In comparison to the control, the d H of IgG has not significantly changed in the presence of formulations F1, F2, and F3.…”

Section: Resultssupporting

confidence: 82%

Impact of ZIF-8, ArgHCl, and Ionic Liquid-Based Formulations on the Conformational and Colloidal Stability of Antibodies

et al. 2023

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Various formulations consisting of biomaterials zirconium imidazolate framework-8 (ZIF-8), choline acetate ([Ch][Ac]), and arginine hydrochloride (argHCl) are optimized to study the stability of antibody, Immunoglobulin G (IgG). We have performed several instrumentations including UV−visible spectroscopy, dynamic light scattering (DLS), circular dichroism (far UV CD), and atomic force microscopy (AFM) in the presence of all the formulations to investigate the conformational and colloidal stability of the antibodies. Alongside, the packing efficiency of all the formulations was also explored by storing IgG at 4 °C for 3 months. We have tried to investigate the interactions between biomaterials and antibodies with the motive of designing aggregation-resistant formulations. The overall stability of IgG was improved in the presence of [Ch][Ac]; however, ZIF-8 and argHCl cause relatively more aggregation, although the structure was retained in all the formulations. The key aspect of this study is that the presence of [Ch][Ac] increases ZIF-8@IgG's thermal stability and resistance to IgG-argHCl aggregation. All over, for the first time, with different experimental approaches, the impact of each biomaterial individually and in combination is explored to study their effect on the stability of antibodies. Thus, better efficient formulations can be designed for the storage/packaging of IgG-based drugs which ultimately will have more applicability in pharmaceuticals.

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Section: Resultssupporting

confidence: 82%

Impact of ZIF-8, ArgHCl, and Ionic Liquid-Based Formulations on the Conformational and Colloidal Stability of Antibodies

et al. 2023

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“…prepared cholinium‐based eutectic systems for improving the conformational and colloidal stability of IgG antibodies without adding any excipients. Furthermore, the IgG exhibited a relatively long‐term stability (20 days) at rt [135] . Altogether, these studies demonstrate that DESs are promising solvents for many therapeutic molecules and can serve as vehicles for transdermal delivery of therapeutic proteins.…”

Section: Role Of Deep Eutectic Solvents In Drug Engineeringmentioning

confidence: 64%

“…Furthermore, the IgG exhibited a relatively long-term stability (20 days) at rt. [135] Altogether, these studies demonstrate that DESs are promising solvents for many therapeutic molecules and can serve as vehicles for transdermal delivery of therapeutic proteins. Table 6 gives some examples about the improvement of solubility, stability, and the therapeutic outcomes of several APIs and biomacromolecules dissolved in DESs.…”

Section: Des As a Solvent For Active Pharmaceutical Ingredientsmentioning

confidence: 87%

Deep Eutectic Solvents: An Eco‐friendly Design for Drug Engineering

et al. 2023

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In the spirit of circular economy and sustainable chemistry, the use of environmentally friendly chemical products in pharmacy has become a hot topic. In recent years, organic solvents have been the subject of a great range of restriction policies due to their harmful effects on the environment and toxicity to human health. In parallel, deep eutectic solvents (DESs) have emerged as suitable greener solvents with beneficial environmental impacts and a rich palette of physico‐chemical advantages related to their low cost and biocompatibility. Additionally, DESs can be accompanied by a remarkable solubilizing effect for several active pharmaceutical ingredients (APIs), thus forming therapeutic DESs (TheDESs). In this work, special attention is paid to DESs, presenting a precise definition, classification, methods of preparation, and characterization. A description of natural DESs (NaDESs), i.e., eutectic solvents present in natural sources, is also reported. Moreover, the present review article is the first one to detail the different approaches for judiciously selecting the constituents of DESs in order to minimize the number of experiments. The role of DESs in the biomedical and pharmaceutical sectors and their impact on the development of successful therapies are also discussed.

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“…Arora et al 66 deliberated on maintaining the structural and thermal stability of hemoglobin protein using choline chloride-based DESs and concluded that DESs are potential green, non-harmful and substantial solvents that improve the thermal and structural stabilities of hemoglobin. Very recently, Dhiman et al 67 unveiled the potential use of DESs to enhance the colloidal and conformational stabilities of immunoglobulin G antibodies. Park et al 68 studied the stabilizing effects of DESs on the activity and structure of lysozyme protein.…”

Section: Gokana Mohana Ranimentioning

confidence: 99%

Deep eutectic solvents induced changes in the phase transition behavior of smart polymers: a sustainable future approach

Umapathi

Ghoreishian

Kumar

et al. 2023

Phys. Chem. Chem. Phys.

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Unveiling the potential of deep eutectic solvents to improve the conformational and colloidal stability of immunoglobulin G antibodies

Abstract: Deep Eutectic solvent as a suitable solvent system to design a final pharmaceutical formulation of immunoglobulin G antibodies.

Cited by 11 publications

References 60 publications

Impact of ZIF-8, ArgHCl, and Ionic Liquid-Based Formulations on the Conformational and Colloidal Stability of Antibodies

Impact of ZIF-8, ArgHCl, and Ionic Liquid-Based Formulations on the Conformational and Colloidal Stability of Antibodies

Deep Eutectic Solvents: An Eco‐friendly Design for Drug Engineering

Deep eutectic solvents induced changes in the phase transition behavior of smart polymers: a sustainable future approach

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