Unveiling Cells’ Local Environment during Cryopreservation by Correlative <i>In Situ</i> Spatial and Thermal Analyses

Qin, Kankan; Eschenbrenner, Corentin; Ginot, Félix; Dedovets, Dmytro; Coradin, Thibaud; Deville, Sylvain; Fernandes, Francisco M.

doi:10.1021/acs.jpclett.0c01729

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…Soft objects can deform (and heal or not), coalesce, grow, shrink, or lose their integrity (cells) upon interacting with the front. Their survival and functionality in the final complex composites are directly related to their physicochemical environment during freezing, as we recently reported with yeast cells frozen in the presence of alginate. The list of questions that must be addressed expands rapidly.…”

Section: Current Trends and Possible Futuresmentioning

confidence: 53%

Complex Composites Built through Freezing

2022

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Conspectus Using a limited selection of ordinary components and at ambient temperature, nature has managed to produce a wide range of incredibly diverse materials with astonishingly elegant and complex architectures. Probably the most famous example is nacre, or mother-of-pearl, the inner lining of the shells of abalone and certain other mollusks. Nacre is 95% aragonite, a hard but brittle calcium carbonate mineral, that exhibits fracture toughness exceedingly greater than that of pure aragonite, when tested in the direction perpendicular to the platelets. No human-made composite outperforms its constituent materials by such a wide margin. Nature’s unique ability to combine the desirable properties of components into a material that performs significantly better than the sum of its parts has sparked strong interest in bioinspired materials design. Inspired by this complex hierarchical architecture, many processing routes have been proposed to replicate one or several of these features. New processing techniques point to a number of laboratory successes that hold promise in mimicking nacre. We pioneered one of them, ice templating, in 2006. When a suspension of particles is frozen, particles are rejected by the growing ice crystals and concentrate in the space between the crystals. After the ice is freeze-dried, the resulting scaffold is a porous body that can eventually be pressed to increase the density and then be infiltrated with a second phase, providing multilayered, lamellar complex composites with a microstructure reminiscent of nacre. The composites exhibit a marked crack deflection during crack propagation, enhancing the damage resistance of the materials, offering an interesting trade-off of strength and toughness. Freezing as a path to build complex composites has turned out to be a rich line of research and development. Understanding and controlling the freezing routes and associated phenomena has become a multidisciplinary endeavor. A step forward in the complexity was achieved with the use of anisotropic particles. Ice-induced segregation and alignment of platelets can yield dense, inorganic composites (nacre-like alumina) with a complex architecture and microstructure, replicating several of the morphological features of nacre. Now, a different class of complex composites is quickly arising: engineered living materials, developed in the soft matter and biology communities. The material-agnostic nature of the freezing routes, the use of an aqueous system, the absence of organic solvents, and the low temperatures being used are all strong assets for the development of such complex composites. More complex building blocks, such as cells or bacteria, can be frozen. Understanding the fundamental mechanisms controlling the interactions between the ice crystals and the objects as well as the interactions between the soft objects themselves and their fate is essential in this context. In this Account, we highlight our efforts over the past decade to achieve the controlled synthesis of nacre-like composites...

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Section: Current Trends and Possible Futuresmentioning

confidence: 53%

Complex Composites Built through Freezing

2022

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“…Part of the water remains unfrozen and bound to the solute in a vitreous or glass-like phase 192,193 . Ice can adopt different morphologies that range from fully isotropic up to highly anisotropic lamellar crystals 190 .…”

Section: Controlled Porosity Through Freezingmentioning

confidence: 99%

“…These parameters can be set to encourage cell infiltration and suitable mechanical properties for tissue regeneration 201 . Numerous methods have been developed to control ice formation and growth, including static copper platform 202 , rotational freezing 196 , controlled dipping 195,203 , and translational plane freezing 193,204 . These methods offer to control the macroscopic geometry of the scaffolds (by the design of relevant molds) and, simultaneously allow for a fine adjustment of the macroporosity within the materials via a set of well-defined thermal boundary conditions.…”

Section: Controlled Porosity Through Freezingmentioning

confidence: 99%

Biomimetic materials to replace tubular tissues

Martinier,

Trichet,

Fernandes

2024

Preprint

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Repairing tubular tissues—the trachea, the esophagus, urinary and gastrointestinal tracts, and the circulatory system—from trauma or severe pathologies that require resection, calls for new, more effective graft materials. Currently, the relatively narrow family of materials available for these applications relies on synthetic polymers that fail to reproduce the biological and physical cues found in native tissues. Mimicking the structure and the composition of native tubular tissues to elaborate functional grafts is expected to outperform the materials currently in use, but remains one of the most challenging goals in the field of biomaterials. Despite their apparent diversity, tubular tissues share extensive compositional and structural features. Here, we assess the current state of the art through a dual layer model, reducing each tissue to an inner epithelial layer and an outer muscular layer. Based on this model, we examine the current strategies developed to mimic each layer and we underline how each fabrication method stands in providing a biomimetic material for future clinical translation. The analysis provided here, addressed to materials chemists, biomaterials engineers and clinical staff alike, sets new guidelines to foster the elaboration of new biomimetic materials for effective tubular tissue repair.

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“…These agents demonstrated comparable ice recrystallization inhibition (IRI) activities. [21][22][23][24][25] For example, 0.1% PVA was observed to slow down the growth of ice crystals and improve post-thaw cell recovery, in the case of sheep and human erythrocytes. 26 The potential of these macromolecules in cell cryopreservation was realized by discovering the cryoprotective activities of polyethylene glycol (PEG) in 1954, and PVP and dextran in 1955.…”

Section: Advait Bhagwatmentioning

confidence: 99%