Unusual sensitization to parvalbumins from certain fish species

Raith, Marianne; Klug, Christoph; Sesztak‐Greinecker, Gabriele; Balic, Nadja; Focke, Margarete; Linhart, Birgit; Hemmer, Wolfgang; Swoboda, Ines

doi:10.1016/j.anai.2014.08.001

Cited by 17 publications

(16 citation statements)

References 7 publications

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“…This study highlights region‐ and species‐specific IgE reactivity to fish, leading possible to false‐negative diagnostics . Species‐specific sensitization was also demonstrated in other studies for salmonids, tilapia, catfish and angler . However, the patient‐ and fish species‐dependant reactivity requires further investigations with a larger patient cohort and region‐specific species.…”

Section: Discussionsupporting

confidence: 72%

Variability of allergens in commercial fish extracts for skin prick testing

Ruethers

Taki

Nugraha

et al. 2019

Allergy

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Background: Commercial allergen extracts for allergy skin prick testing (SPT) are widely used for diagnosing fish allergy. However, there is currently no regulatory requirement for standardization of protein and allergen content, potentially impacting the diagnostic reliability of SPTs. We therefore sought to analyse commercial fish extracts for the presence and concentration of fish proteins and in vitro IgE reactivity using serum from fish-allergic patients.Methods: Twenty-six commercial fish extracts from five different manufacturers were examined. The protein concentrations were determined, protein compositions analysed by mass spectrometry, followed by SDS-PAGE and subsequent immunoblotting with antibodies detecting 4 fish allergens (parvalbumin, tropomyosin, aldolase and collagen). IgE-reactive proteins were identified using serum from 16 children with confirmed IgE-mediated fish allergy, with focus on cod, tuna and salmon extracts. Results:The total protein, allergen concentration and IgE reactivity of the commercial extracts varied over 10-fold between different manufacturers and fish species. The major fish allergen parvalbumin was not detected by immunoblotting in 6/26 extracts. In 7/12 extracts, five known fish allergens were detected by mass spectrometry. For cod and tuna, almost 70% of patients demonstrated the strongest IgE reactivity to collagen, tropomyosin, aldolase A or β-enolase but not parvalbumin.

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Section: Discussionsupporting

confidence: 72%

Variability of allergens in commercial fish extracts for skin prick testing

Ruethers

Taki

Nugraha

et al. 2019

Allergy

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“…Patients with monosensitization to salmonid fishes have specific IgE antibodies recognizing the salmonid parvalbumins only . A similar case has been reported for food allergy to pangasius . Clinical reactivity to fish is not necessarily linked to the presence of specific IgE to parvalbumins.…”

mentioning

confidence: 62%

Cross‐reactivity to fish and chicken meat – a new clinical syndrome

Kuehn

Codreanu‐Morel

Lehners‐Weber

et al. 2016

Allergy

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“…In doubt, an oral food challenge test can clarify cases of negative or low IgE to Ara h 2. If no specific IgE to any seed storage protein can be identified, a clinically relevant peanut allergy is unlikely, although it cannot be ruled out completely in the presence of sufficient clinical suspicion (diagnostic gap due to, e.g., oleosins (Ara h 10/11, 14/15) and defensins (Ara h 12/13) ). Specific IgE to the nsLTP Ara h 9 should be additionally determined in patients with systemic reactions but negative to the storage proteins of peanut.…”

Section: Clinical Relevance Diagnosis and Managementmentioning

confidence: 99%

EAACI Molecular Allergology User's Guide

Matricardi

Kleine-Tebbe²,

Hoffmann

et al. 2016

Pediatric Allergy Immunology

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870

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The availability of allergen molecules ('components') from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled 'component-resolved diagnosis' (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology User's Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, milk, egg, meat, fish, and shellfish), inhalants (pollen, mold spores, mites, and animal dander), and Hymenoptera venom. Diagnostic algorithms and short case histories provide useful information for the clinical workup of allergic individuals targeted for CRD. Part C covers protein families containing ubiquitous, highly cross-reactive panallergens from plant (lipid transfer proteins, polcalcins, PR-10, profilins) and animal sources (lipocalins, parvalbumins, serum albumins, tropomyosins) and explains their diagnostic and clinical utility. Part D lists 100 important allergen molecules. In conclusion, IgE-mediated reactions and allergic diseases, including allergic rhinoconjunctivitis, asthma, food reactions, and insect sting reactions, are discussed from a novel molecular perspective. The EAACI MAUG documents the rapid progression of molecular allergology from basic research to its integration into clinical practice, a quantum leap in the management of allergic patients.

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Unusual sensitization to parvalbumins from certain fish species

Cited by 17 publications

References 7 publications

Variability of allergens in commercial fish extracts for skin prick testing

Variability of allergens in commercial fish extracts for skin prick testing

Cross‐reactivity to fish and chicken meat – a new clinical syndrome

EAACI Molecular Allergology User's Guide

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