Unusual profile of leukocyte recruitment in mice induced by a skin secretion of the tree frog Phyllomedusa hypochondrialis

Conceição, Kátia; Bruni, Fernanda Miriane; Pareja-Santos, Alessandra; Antoniazzi, Marta Maria; Jared, Carlos; Lopes-Ferreira, Mônica; Lima, Carla; Pimenta, Daniel C.

doi:10.1016/j.toxicon.2006.10.009

Cited by 8 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies by our group reported that the skin secretion of P. hypochondrialis (although we now know that we were working with P. nordestina at the time) induces increased leukocyte rolling flux followed by a gradual increase in cells firmly adherent to the endothelium in intravital experiments (25). Taking our previous results together with the present findings (characterization of BRP), one can conclude that they corroborate the previously described local inflammatory effect of P. nordestina skin administration in mice.…”

Section: Discussionsupporting

confidence: 92%

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Conceição¹,

Bruni²,

Sciani³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

ABSTRACT:Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrinetype serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was prefractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was possible to identify three novel bradykininrelated peptides, namely: KPLWRL-NH 2 (Pnor 3), RPLSWLPK (Pnor 5) and VPPKGVSM (Pnor 7) presenting vascular activities as assessed by intravital microscopy. Pnor 3 and Pnor 7 were able to induce vasodilation. On the other hand, Pnor 5 was a potent vasoconstrictor. These effects were reproduced by their synthetic analogues.

show abstract

Section: Discussionsupporting

confidence: 92%

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Conceição¹,

Bruni²,

Sciani³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…In our study, the effects of SEPr in the formalin test showed that the extract acts by inhibiting both the acute and inflammatory phases of formalin-induced nociception. In contrast, previous studies have shown that the crude skin extract of animals from the Phyllomedusinae subfamily alone, such as the Phyllomedusa hypochondrialis, injected directly in the paw induce nociception and increase inflammatory parameters, such as leukocyte migration and vascular permeability [ 21 , 22 ]. Locomotor activity was also compromised by systemic administration of P. hypochondrialis skin extract [ 22 ], corroborating previous work from Daly et al [ 11 ] with crude skin extract from P. bicolor.…”

Section: Discussionmentioning

confidence: 98%

Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura)

Malpezzi-Marinho

Zanoni

Molska

et al. 2020

Toxins

View full text Add to dashboard Cite

Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus Phyllomedusa represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of Phyllomedusa rohdei in rodent models of pain. The crude skin extract of P. rohdei was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE2 (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of P. rohdei exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE2-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of P. rohdei skin extract as a new therapeutic tool in pain management.

show abstract

“…The Hylidae family has hundred of species that have been extensively studied mainly due to the hallucinogenic properties of their skin secretion and constituents. In fact, several molecules have been described in frog skins (Broccardo et al, 1981;Conceição et al, 2006Conceição et al, , 2007aConceição et al, , 2007bLeite et al, 2005;Mor and Nicolas, 1994), most of them with analgesic and antimicrobial effects. Most of these studies are focused on the isolation of peptides and evaluation of their biological and pharmacological properties.…”

Section: Discussionmentioning

confidence: 99%

“…Activity against grampositive and gram-negative bacteria, yeast and fungi were reported for dermaseptins (Mor et al, 1991(Mor et al, , 1994, while antibacterial activity and antiparasitic activity against Trypanosoma cruzi were demonstrated for phylloseptins (Leite et al, 2005). In addition to these reports, studies dedicated to characterize themain biological effects of crude P. hypochondrialis skin secretion showed that, at low doses, it is able to induce edema and inflammation in the cremaster mice (Conceição et al, 2007a). In addition, the same research team also observed pain, edema, and necrosis, 48 h after intraperitoneal injection in mice (personal communication).…”

Section: Introductionmentioning

confidence: 99%

Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Neiva

Vargas

Conceição

et al. 2013

Toxicon

View full text Add to dashboard Cite

The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. The marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. The transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution.

show abstract

Unusual profile of leukocyte recruitment in mice induced by a skin secretion of the tree frog Phyllomedusa hypochondrialis

Cited by 8 publications

References 25 publications

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura)

Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Contact Info

Product

Resources

About