Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Conceição, Kátia; Bruni, FM; Sciani, Juliana Mozer; Konno, Katsuhiro; Melo, R. I.; Antoniazzi, MM; Jared, C; Lopes-Ferreira, Mônica; Pimenta, Daniel C.

doi:10.1590/s1678-91992009000400004

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…These bradykinin-like related peptides (BRPs) transcripts identified here may possibly contribute for the increased permeability and vasodilatation leading to edematogenic process. These findings were supported by a recent report of two novel BRPs isolated from P. nordestina skin secretion, which were able to induce vasodilatation (Conceição et al, 2009).…”

Section: Discussionsupporting

confidence: 79%

“…A high structural diversity of BRPs in the skin secretions of frogs and toads was described (Chen et al, 2011). The pharmacological effects induced by BRPs include antagonism of bradykinin effects on smooth muscle, vasodilatation, vasoconstriction, and hyperalgesia (Conceição et al, 2009;Picolo et al, 2010;Zhou et al, 2009). Two singlet sequences showing similarity to BRPs, coined as BK01 and BK02, were found in our database.…”

Section: Tryptophyllinsmentioning

confidence: 91%

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Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Neiva

Vargas

Conceição

et al. 2013

Toxicon

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The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. The marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. The transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution.

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Section: Discussionsupporting

confidence: 79%

Section: Tryptophyllinsmentioning

confidence: 91%

Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Neiva

Vargas

Conceição

et al. 2013

Toxicon

View full text Add to dashboard Cite

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“…After the discovery of these first BPPs, similar proline-rich peptides were isolated from different snake venoms [31–34] and even frogs skin secretion [35, 36]. …”

Section: Discussionmentioning

confidence: 99%

“…The first ever described BPP, isolated from Bothrops jararaca venom, became the precursor for the development of anti-hypertensive drugs, such as Captopril® and Lisinopril® [ 22 ]. After the discovery of these first BPPs, similar proline-rich peptides were isolated from different snake venoms [ 31 – 34 ] and even frogs skin secretion [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

Fucase

Sciani

Viala

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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BackgroundVenoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros.MethodsThe crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (<7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation.ResultsTypical BPP signatures were identified in three RP-HPLC fractions. CID fragmentation presented the usual y-ion of the terminal P-P fragment as a predominant signal at m/z 213.1. De novo peptide sequencing identified one Bothrops-like BPP and one new BPP sequence. The new BPP was synthesized and showed poor inhibition over ACE, but displayed significant bradykinin-induced edema potentiation.ConclusionsSo far, few BPPs are described in Viperinae, and based on the sequenced peptides, two non-canonical sequences were detected. The possible clinical role of this new peptides remains unclear.

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“…Although this study focused on venom gland secretions primarily, poisonous animal lineages with apocrine or holocrine secretion methods (e.g., amphibians) might be similarly harnessed to generate the transcriptome of genome-derived compounds (i.e. proteins and peptides) in the poison glands [74][75][76][77]100].…”

Section: Plos Onementioning

confidence: 99%

A non-lethal method for studying scorpion venom gland transcriptomes, with a review of potentially suitable taxa to which it can be applied

et al. 2021

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Scorpion venoms are mixtures of proteins, peptides and small molecular compounds with high specificity for ion channels and are therefore considered to be promising candidates in the venoms-to-drugs pipeline. Transcriptomes are important tools for studying the composition and expression of scorpion venom. Unfortunately, studying the venom gland transcriptome traditionally requires sacrificing the animal and therefore is always a single snapshot in time. This paper describes a new way of generating a scorpion venom gland transcriptome without sacrificing the animal, thereby allowing the study of the transcriptome at various time points within a single individual. By comparing these venom-derived transcriptomes to the traditional whole-telson transcriptomes we show that the relative expression levels of the major toxin classes are similar. We further performed a multi-day extraction using our proposed method to show the possibility of doing a multiple time point transcriptome analysis. This allows for the study of patterns of toxin gene activation over time a single individual, and allows assessment of the effects of diet, season and other factors that are known or likely to influence intraindividual venom composition. We discuss the gland characteristics that may allow this method to be successful in scorpions and provide a review of other venomous taxa to which this method may potentially be successfully applied.

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Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

Cited by 12 publications

References 26 publications

Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

A non-lethal method for studying scorpion venom gland transcriptomes, with a review of potentially suitable taxa to which it can be applied

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