Untersuchungen über Getreideschleimstoffe. I. Chromatographische Fraktionierung von wasserlöslichen Weizenmehlpentosanen an Diäthylaminoäthyl‐Cellulose

Abstract

Volumen XLIV, Fasciculus 111 (1961) -No. 93-94 823 SUMMARY 1. The rates of the following nucleophilic substitution reactions have been measured in benzene solution : (1) cyanuric chloride and aniline ; (2) 2-anilino-3,5-dichloro-triazine and aniline ; (3) l-N-methylanilino-3,5-dichloro-triazine and N-methylaniline.2. Reaction (1) ist autocatalysed; it is first order with respect to cyanuric chloride and approximatively first order with respect to aniline (weak base catalysis by aniline).3. Reaction (2) is also autocatalys-d. I t is second o r d x with respect to l-anilino-3,5-dichloro-triazine and approximatively first order with respect to aniline.

A Water-soluble Arabinogalactan-Peptide From Wheat Endosperm

“…The polysaccharides in water extracts of Insignia wheat flour prepared by the modified methods of Kundig et al (1961) (preparation A) and Preece and MacDougall (1958) (preparation B) have similar monosaccharide compositions (Table 2) and molecular size distributions (Fig. 1).…”

“…The demonstration of the presence of free protein in preparation A suggests that some of the protein eluted by the stepwise increase of sodium borate concentration from the DEAE-cellulose column of Kundig et al (1961), and designated glycoprotein by these workers, is also free protein.…”

“…The first (method 1, Table 1) was a modification of the procedure of Kundig et al (1961). This procedure differed from that of Kundig and co-workers in that human salivary amylase was substituted for porcine pancreatic amylase Table 1.…”

“…This procedure differed from that of Kundig and co-workers in that human salivary amylase was substituted for porcine pancreatic amylase Table 1. Procedures for extraction of water-soluble polysaccbarides Method 1 based on the method of Kundig et al (1961) and method 2 on that of Preece and MacDougall (1958) and after starch digestion the amylase was removed by heat denaturation rather than by trichloroacetic acid precipitation. The use of trichloroacetic acid was avoided to minimize possible chemical degradation (e.g.…”

Chemical Analysis and Inhibition Reactions of the Group and Type Antigens of Group F Streptococci

“…The galactose determination was corrected for the presence of the previously determined amounts of glucose and rhamnose; the mannose determination was corrected for the presence of the previously determined amounts of glucose, galactose and rhamnose, Anion exchange chromatography. Column chromatography of formamide extracts was carried out on DEAE-cellulose (Serva, Heidelberg), as described by Kundig, Neukom & Deuel (1961) for cereals. The column was eluted with the following solutions: distilled water, phosphate buffer pH 7, 0.0175 M (PB), PB+O-1 M-NaCl, PB +0.25 M-NaCl, 0.1 N-NaOH.…”