Untersuchungen über die Jaffesche Pikrinsäurereaktion.

Weise, W.; Tropp, Caspar

doi:10.1515/bchm2.1928.178.1-3.125

Cited by 13 publications

(5 citation statements)

References 3 publications

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“…Approximate E,/ 2 and I d measurements are presented. 4 A zero time blank was withdrawn immediately after mixing. Results of the acid/alkali treatment procedure for alkaline picrate blank solutions, and 1:1, 2:1, 5:1 and 10:1 glucose: picrate test solutions have been tabulated (see tab.…”

Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%

“…Approximate Ej/ 2 and I d measurements are presented. 4 A zero time blank was withdrawn immediately after mixing. 5 Capillary was clogged by precipitate.…”

Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%

“…Approximate E 1/2 and I d measurements are presented. 4 A zero time blank was withdrawn immediately after mixing. The IR spectrum of the reddish-brown coloured product separated by TLC was identical to the IR spectrum obtained for the purified picramic acid.…”

Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%

“…warming, an immediate blood red-coloured reduction Jaffe reported in 1886 that a red colour formed at P^duct of picric acid formed Other investigators had room temperature when creatinine was reacted with also reported that a red colour formed upon the ν ν ο Ά + 11 r ττ ΛΙ\ τν/τ Λ-f Α τ Λ" application of heat (2,4,12,(15)(16)(17)(18)(19) the cold, glucose gave a positive reaction with alkaline procedure for the measurement of creatinine in biof · , logical samples. However, dependent upon the reac-p ; crate -At room *****»?…”

Section: Introductionmentioning

confidence: 99%

“…Τ ί } ΐ *, 8 " ΡΡ ? 8δ10η °f acetone (4,5), acetoacetate (3,6,7), NaOH (8), bili-°° °U r an f d rep°rted that the sum of the chro ™g en rubin (9), protein (10,11), glucose (5,(12)(13)(14), etc. values of a solutl°,n f pure ^u cose and of pure creatinine, separately determined, was greater than Jaffe showed that glucose did not react with alkaline the total chromogen concentration of the same quanpicrate at ambient temperature, except after an ex-tities of the two in combination.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Glucose upon Alkaline Picrate: A Jaffé Interference

Viraraghavan¹,

Blass²

1990

Clinical Chemistry and Laboratory Medicine

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Summary:The reactivity of glucose in aqueous alkaline picrate was investigated by spectrophotometry and polarography at 25 °C in 0.51 mol/1 sodium hydroxide. Thin-layer chromatography and infrared spectroscopy studies have conclusively identified the presence of picramic acid in 5:1 and 10:1 glucose picrate test solutions incubated at 25 °C.The polarographic data of an alkaline picrate blank with a concentration of 0.284 mmol/1, show three welldefined nitro group reduction waves with approximate half-wave potentials of -0.62 V, -0.78 V, and -0.93 V and a fourth broad wave appearing near -1.31 V versus a saturated calomel electrode. The addition of glucose to alkaline picrate resulted in a decreased diffusion current for reduction waves 1-3, with little change in reduction wave 4. The reactivity of test solutions containing glucose: picrate in 1:1, 2:1, 5:1 and 10:1 molar ratios was investigated at varied time intervals between 10 and 180 minutes.The absorption spectra of a 10:1 glucose: picrate solution shifted from 356 nm to 375 nm and a broad tailing shoulder absorbance formed in the 450-600 nm region. An orange coloured minor product, separated by thin-layer chromatography, was observed to fluoresce. The maximum excitation and emission wavelengths were 318 nm and 545 nm, respectively. A major, red-coloured product was isolated and identified as picramic acid by infrared spectroscopy. For 10:1 glucose: picrate test solutions incubated at 25 °C, picramic acid formed within 10 minutes. Within the first minute, the colour was observed to change from yellow to orange and then to red.

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Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%

“…Approximate Ej/ 2 and I d measurements are presented. 4 A zero time blank was withdrawn immediately after mixing. 5 Capillary was clogged by precipitate.…”

Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%

Section: Preparation Of Glucose : Picrate Reaction Productsmentioning

confidence: 99%