In many reactions proceeding in living cells the concentration of the reactants changes with time, and hence our knowledge of the nature of the mechanism cannot proceed very far without means of determining, directly or indirectly, the changes of concentration. In photosynthesis the reactants carbon dioxide and water can often be readily maintained at a constant level, and consequently investigations have been centred on the relation between the rate of photosynthesis, when it has reached a stationary value, and the con centration of the reactants and various inhibitors and accelerators. Transitions from one stationary state to another, consequent upon a change in intensity of one of the determining factors, have received little attention. Investigation of such transitions may throw light on the mechanism of the process itself, and possibly on the interaction of this and other processes in the cell. The transition to be considered in this paper is that taking place in the rate of photosynthesis when chlorophyll-containing cells pass from darkness to