Untersuchungen an Glycerinphospholipiden aus wei�er Substanz von MS- und Normalgehirnen

Winterfeld, Manfred; Debuch, Hildegard

doi:10.1007/bf00312497

Cited by 20 publications

(10 citation statements)

References 30 publications

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“…Since autopsy brain materials from 10 MS patients were available to us, the present communication was undertaken as an attempt to clarify some of the above-mentioned uncertainties. In continuation of our previous observations on white matter (Winterfeld and Debuch, 1977), we will now give data on the composition of MS myelin compared with that of 10 normal controls. The CNPase assay within the same myelin samples may serve as a further criterion for their purity.…”

mentioning

confidence: 76%

“…FAME from samples of PC, PE, and PS after twodimensional tlc (see below) as well as DMA from P E fractions were obtained as described by Winterfeld and Debuch (1977).…”

Section: Preparation Of Fame and Dmamentioning

confidence: 99%

“…Neutral lipids were separated by one-dimensional tlc (solvent system: petroleum ether/ether/acetic acid, 80:20:2 by vol. ), phospholipids by two-dimensional tlc, and were analysed a s described by Winterfeld and Debuch (1977).…”

Section: Chromatographymentioning

confidence: 99%

“…Assuming that in the brains of MS patients alterations of the lipids of the 'normal-appearing' white matter may occur, many investigators have looked for such differences in this material. While Cumings and Goodwin (1968), Arnetoli et al (1969), Gerstl et al (1970), and Woelk and Borri (1973~) (for further references see Winterfeld and Debuch, 1977) found a depletion of the total lipid content and changes in the individual lipid classes and/or in the fatty acid pattern of the normal-appearing white matter of MS, only slight or no alterations were detected by Kishirnoto et al (1967) and by Alling et al (1971) in these tissues.…”

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confidence: 94%

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2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

Göpfert

Pytlik

Debuch

1980

Journal of Neurochemistry

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A study of purified myelin samples from normal‐appearing white matter of 10 multiple sclerosis (MS) brains was undertaken and the results were compared with 10 age‐matched control brains. Statistical evaluations were carried out with Student's r‐test for differences. In pathological samples the yield of myelin came to only two‐thirds of the corresponding controls. Enzyme assays of the 2′, 3′‐cyclic 3′‐phosphohydrolase revealed an obviously significant reduction of specific activity to one‐half in MS myelins. In myelin the contents of protein, lipid classes as cholesterol, glycolipids and phospholipids did not differ significantly. No cholesterol esters or any lysophospholipid were detectable either in MS or in controls. Within the individual phospholipids the main components were in the same order, while a significant decrease of the acidic representatives and of sphingomyelin occurred. Analysis of the fatty acid pattern of phosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidylethanolamine (PE), including the aldehydes from the last, revealed quite similar values with no significant differences, except C22: 4 fatty acid in the PE fraction and C20: 1 fatty acid in PS, which were reduced in MS myelin samples.

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mentioning

confidence: 76%

“…FAME from samples of PC, PE, and PS after twodimensional tlc (see below) as well as DMA from P E fractions were obtained as described by Winterfeld and Debuch (1977).…”

Section: Preparation Of Fame and Dmamentioning

confidence: 99%

Section: Chromatographymentioning

confidence: 99%

mentioning

confidence: 94%

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2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

Göpfert

Pytlik

Debuch

1980

Journal of Neurochemistry

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“…A number of investigators have reported decreased total lipid (Cumings, 1953(Cumings, , 1955Davison and Wajda, 1962;Gerstl el al., 1961Gerstl el al., , 1963Gerstl el al., , 1970Kishimoto el al., 1967;Einstein el al., 1970;Woelk and Borri, 1973a;Winterfeld and Dubuch, 1977), decreased phospholipid, particularly ethanolamine plasmalogens (Gerstl el al., 1970;Yanagihara and Cumings, 1969) and acidic phospholipids (Gerstl el al., 1970;Alling el al., 1971;Woelk and Borri, 1973a;Winterfeld and Debuch, 1977;Neu and Woelk, 1982), and los ses of cerebroside (Cumings, 1953;Einstein el al., 1979;Gerstl el al., 1970) and sulfatide (Alling el al., 1971;Woelk and Borri, 1973a;Neu and Woelk, 1982). Cholesterol esters are frequently found (Cumings, 1953(Cumings, , 1955Davison and Wajda, 1962;Alling et al, 1971;Cuzner and Davison, 1973;Wender et al, 1973), and their level correlates with a reduction in the activities of cholesterol ester hydrolases (Shah and Johnson, 1980).…”

Section: B Normal-appearing White Mattermentioning

confidence: 99%

Myelin

1984

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Biochemical alterations in multiple sclerosis lesions and normal‐appearing white matter detected by in vivo ³¹P and ¹H spectroscopic imaging

Husted

Goodin

Hugg

et al. 1994

Annals of Neurology

203

106

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The goals of the current study were threefold: first, to confirm previous single volume proton (1H) magnetic resonance spectroscopy results of reduced N-acetyl aspartate (NAA, a putative marker of neurons) in multiple sclerosis (MS) white matter lesions using multiple volume 1H magnetic resonance spectroscopic imaging (MRSI); second, to measure the phospholipid metabolites phosphomonoesters and phosphodiesters in such lesions using phosphorus (31P) MRSI; and third, to test the hypothesis that biochemical changes occur in the normal-appearing (on spin echo T2-weighted magnetic resonance images) white matter in patients with MS. Thirteen subjects with clinically definite MS were studied with both 1H and 31P MRSI, and 19 controls were studied with either 1H MRSI, 31P MRSI, or both. MS lesion, MS normal-appearing white matter, and region-matched control spectra from the centrum semiovale were analyzed. The major findings of this study were that in both white matter lesions and normal-appearing white matter in patients with MS, the metabolite ratio NAA/creatine and the total 31P peak integrals were significantly reduced compared with controls. In addition, in MS lesions NAA/choline and phosphodiesters/total 31P were significantly reduced compared with controls, and in MS normal-appearing white matter there was a trend for NAA/choline to be reduced compared with controls. In normal-appearing white matter in patients with MS, total creatine and phosphocreatine were significantly increased compared to controls, as detected with both 1H (total creatine peak integrals) and 31P (phosphocreatine/total 31P) MRSI techniques. These results suggest reduced neuronal density and altered phospholipid metabolites in white matter lesions in patients with MS.(ABSTRACT TRUNCATED AT 250 WORDS)

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Untersuchungen an Glycerinphospholipiden aus wei�er Substanz von MS- und Normalgehirnen

Cited by 20 publications

References 30 publications

2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

Myelin

Biochemical alterations in multiple sclerosis lesions and normal‐appearing white matter detected by in vivo ³¹P and ¹H spectroscopic imaging

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Untersuchungen an Glycerinphospholipiden aus wei�er Substanz von MS- und Normalgehirnen

Cited by 20 publications

References 30 publications

2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains

Myelin

Biochemical alterations in multiple sclerosis lesions and normal‐appearing white matter detected by in vivo 31P and 1H spectroscopic imaging

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Product

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Biochemical alterations in multiple sclerosis lesions and normal‐appearing white matter detected by in vivo ³¹P and ¹H spectroscopic imaging