Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung

Wang, Zijing; Ma, Peng; Wang, Yisa; Hou, Biyu; Zhou, Can; Tian, He; Li, Bowen; Shui, Guanghou; Yang, Xiuying; Qiang, Guifen; Yin, Chengqian; Du, Guanhua

doi:10.1038/s41598-021-92779-8

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…Among the significantly influenced pathways, glutathione metabolism was closely related to OS and ER stress. 52,53 The increased GPX3 expression in the enriched glutathione metabolism pathway was in line with the elevated hepatic GSH-Px concentration obtained by biochemical analyses in the current study. Moreover, the expression of ACOX1 in the peroxisome pathway was decreased by DEX treatment.…”

Section: ■ Discussionsupporting

confidence: 87%

Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

Yuan

Liu

Zhao

et al. 2023

J. Agric. Food Chem.

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Endoplasmic reticulum (ER) stress plays important roles in oxidative stress (OS), contributing to liver injury. Lactiplantibacillus plantarum P8 (P8) was reported to regulate broiler OS and the gut microbiota in broilers, but its roles in hepatic ER stress remain unclear. In the present study, the role of P8 in liver OS and ER stress was evaluated, and proteomics was performed to determine the mechanism. Results revealed that P8 treatment decreased liver OS and ER stress in dexamethasone (DEX)-induced oxidatively stressed broilers. Proteomics showed that differentially expressed proteins (DEPs) induced by DEX cover the "cellular response to unfold protein" term. Moreover, the DEPs (GGT5, TXNDC12, and SRM) between DEX-and DEX + P8-treated broilers were related to OS and ER stress and enriched in the glutathione metabolism pathway. RT-qPCR further confirmed the results of proteomics. In conclusion, P8 attenuates hepatic OS and ER stress by regulating GGT5, TXNDC12, SRM, and glutathione metabolism in broilers.

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Section: ■ Discussionsupporting

confidence: 87%

Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

Yuan

Liu

Zhao

et al. 2023

J. Agric. Food Chem.

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“…Metabolomic techniques such as targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) are used for the quantification of specific metabolites like glutathione, directly involved in redox homeostasis [ 82 ]. Untargeted Metabolomics gives a comprehensive overview of metabolic changes due to oxidative stress and can provide potential biomarkers for cancer [ 83 ].…”

Section: Methods For Oxidative Stress Profiling In Oncologymentioning

confidence: 99%

Interplay of oxidative stress, cellular communication and signaling pathways in cancer

Iqbal,

Kabeer,

Abbas

et al. 2024

Cell Commun Signal

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Cancer remains a significant global public health concern, with increasing incidence and mortality rates worldwide. Oxidative stress, characterized by the production of reactive oxygen species (ROS) within cells, plays a critical role in the development of cancer by affecting genomic stability and signaling pathways within the cellular microenvironment. Elevated levels of ROS disrupt cellular homeostasis and contribute to the loss of normal cellular functions, which are associated with the initiation and progression of various types of cancer. In this review, we have focused on elucidating the downstream signaling pathways that are influenced by oxidative stress and contribute to carcinogenesis. These pathways include p53, Keap1-NRF2, RB1, p21, APC, tumor suppressor genes, and cell type transitions. Dysregulation of these pathways can lead to uncontrolled cell growth, impaired DNA repair mechanisms, and evasion of cell death, all of which are hallmark features of cancer development. Therapeutic strategies aimed at targeting oxidative stress have emerged as a critical area of investigation for molecular biologists. The objective is to limit the response time of various types of cancer, including liver, breast, prostate, ovarian, and lung cancers. By modulating the redox balance and restoring cellular homeostasis, it may be possible to mitigate the damaging effects of oxidative stress and enhance the efficacy of cancer treatments. The development of targeted therapies and interventions that specifically address the impact of oxidative stress on cancer initiation and progression holds great promise in improving patient outcomes. These approaches may include antioxidant-based treatments, redox-modulating agents, and interventions that restore normal cellular function and signaling pathways affected by oxidative stress. In summary, understanding the role of oxidative stress in carcinogenesis and targeting this process through therapeutic interventions are of utmost importance in combating various types of cancer. Further research is needed to unravel the complex mechanisms underlying oxidative stress-related pathways and to develop effective strategies that can be translated into clinical applications for the management and treatment of cancer.

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“…Significantly altered metabolites and genes were submitted to joint pathway analysis in Metaboanalyst . Out of the 324 biological processes identified, 74 pathways had P value < 0.05 and FDR < 25%.…”

Section: Resultsmentioning

confidence: 99%

The PCOS–NAFLD Multidisease Phenotype Occurred in Medaka Fish Four Generations after the Removal of Bisphenol A Exposure

Chakraborty

Anand

Coe

et al. 2023

Environ. Sci. Technol.

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As a heterogeneous reproductive disorder, polycystic ovary syndrome (PCOS) can be caused by genetic, diet, and environmental factors. Bisphenol A (BPA) can induce PCOS and nonalcoholic fatty liver disease (NAFLD) due to direct exposure; however, whether these phenotypes persist in future unexposed generations is not currently understood. In a previous study, we observed that transgenerational NAFLD persisted in female medaka for five generations (F4) after exposure to an environmentally relevant concentration (10 μg/L) of BPA. Here, we demonstrate PCOS in the same F4 generation female medaka that developed NAFLD. The ovaries contained immature follicles, restricted follicular progression, and degenerated follicles, which are characteristics of PCOS. Untargeted metabolomic analysis revealed 17 biomarkers in the ovary of BPA lineage fish, whereas transcriptomic analysis revealed 292 genes abnormally expressed, which were similar to human patients with PCOS. Metabolomic–transcriptomic joint pathway analysis revealed activation of the cancerous pathway, arginine–proline metabolism, insulin signaling, AMPK, and HOTAIR regulatory pathways, as well as upstream regulators esr1 and tgf signaling in the ovary. The present results suggest that ancestral BPA exposure can lead to PCOS phenotypes in the subsequent unexposed generations and warrant further investigations into potential health risks in future generations caused by initial exposure to EDCs.

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Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung

Cited by 8 publications

References 45 publications

Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

Interplay of oxidative stress, cellular communication and signaling pathways in cancer

The PCOS–NAFLD Multidisease Phenotype Occurred in Medaka Fish Four Generations after the Removal of Bisphenol A Exposure

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