Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

Abstract

Endoplasmic reticulum (ER) stress plays important roles in oxidative stress (OS), contributing to liver injury. Lactiplantibacillus plantarum P8 (P8) was reported to regulate broiler OS and the gut microbiota in broilers, but its roles in hepatic ER stress remain unclear. In the present study, the role of P8 in liver OS and ER stress was evaluated, and proteomics was performed to determine the mechanism. Results revealed that P8 treatment decreased liver OS and ER stress in dexamethasone (DEX)-induced oxidatively stressed broilers. Proteomics showed that differentially expressed proteins (DEPs) induced by DEX cover the "cellular response to unfold protein" term. Moreover, the DEPs (GGT5, TXNDC12, and SRM) between DEX-and DEX + P8-treated broilers were related to OS and ER stress and enriched in the glutathione metabolism pathway. RT-qPCR further confirmed the results of proteomics. In conclusion, P8 attenuates hepatic OS and ER stress by regulating GGT5, TXNDC12, SRM, and glutathione metabolism in broilers.

Bacillus amyloliquefaciens attenuates the intestinal permeability, oxidative stress and endoplasmic reticulum stress: transcriptome and microbiome analyses in weaned piglets

“…Additionally, the DEGs between the SC06 + DQ and DQ groups were mostly enriched in Arginine biosynthesis, Protein digestion and absorption, Glutathione metabolism, etc. Glutathione metabolism is well-known in redox regulation ( Yuan et al, 2023a ). Arginine, a basic amino acid, serves as an essential precursor for the synthesis of biologically important molecules such as protein, ornithine, proline, polyamines, creatine, NO and agmatine ( Wu and Morris, 1998 ).…”

“…RNA extraction and real-time quantitative PCR (RT-qPCR) were performed according to our previous study ( Yuan et al, 2023a ). In brief, total RNA was extracted from ileal mucosa using Trizol reagent (Tiangen Biochemical Technology Co., Ltd., Beijing, China).…”