The unsteady boundary layer behavior on a turbine cascade is experimentally investigated and the results are presented in this paper. To perform a detailed study on unsteady cascade aerodynamics and heat transfer, a new large-scale, high-subsonic research facility for simulating the periodic unsteady flow has been developed. It is capable of sequentially generating up to four different unsteady inlet flow conditions that lead to four different passing frequencies, wake structures, and freestream turbulence intensities. For a given Reynolds number, three different unsteady wake formations are utilized. Detailed unsteady boundary layer velocity, turbulence intensity, and pressure measurements are performed along the suction and pressure surfaces of one blade. The results presented in the temporal-spatial domain display the transition and further development of the boundary layer, specifically the ensemble-averaged velocity and turbulence intensity.