Unremitting Cell Proliferation in the Secretory Phase of Eutopic Endometriosis: Involvement of pAkt and pGSK3β

Franco-Murillo, Yanira; Miranda-Rodríguez, José Antonio; Rendón-Huerta, Erika P.; Montaño, Luis F.; Cornejo, Gerardo Velázquez; Gomez, L.; Valdez-Morales, Francisco J.; González-Sánchez, Ignacio; Cerbón, Marco

doi:10.1177/1933719114549843

Cited by 17 publications

(11 citation statements)

References 57 publications

(65 reference statements)

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“…Endometrial cell proliferation decreased between the proliferative to mid-secretory phases; however, uncontrolled endometrial epithelial cell proliferation can lead to implantation failure and has been observed in the eutopic secretory endometrium of patients with endometriosis (69). Therefore, endometrial cell proliferation is closely related to pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid‑secretory phases

Kim

Han

et al. 2021

Exp Ther Med

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Successful embryo implantation is the first step for establishing natural pregnancy and is dependent on the crosstalk between the embryo and a receptive endometrium. However, the molecular signaling events for successful embryo implantation are not entirely understood. To identify differentially expressed transcripts [long-noncoding RNAs (lncRNAs), microRNAs (miRNAs) and mRNAs] and competing endogenous RNA (ceRNA) networks associated with endometrial receptivity, the current study analyzed gene expression profiles between proliferative and mid-secretory endometria in fertile women. A total of 247 lncRNAs, 67 miRNAs and 2,154 mRNAs were identified as differentially expressed between proliferative and mid-secretory endometria. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that these differentially expressed genes were significantly enriched for 'cell adhesion molecules.' Additionally, 98 common mRNAs were significantly involved in tryptophan metabolism, metabolic pathways and FoxO signaling. From the differentially expressed lncRNA/miRNA/mRNA ceRNA network, hub RNAs that formed three axes were identified: The DLX6-AS1/miR-141 or miR-200a/OLFM1 axis, the WDFY3-AS2/miR-135a or miR-183/STC1 axis, and the LINC00240/miR-182/NDRG1 axis. These may serve important roles in the regulation of endometrial receptivity. The hub network of the current study may be developed as a candidate marker for endometrial receptivity.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid‑secretory phases

Kim

Han

et al. 2021

Exp Ther Med

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“…41,42 Especially, p-AKT (Ser473) was highly expressed in endometriotic stromal cells, which led to the reduced decidualization and cell survival. 41,43 Therefore, we evaluated the levels of PN, ILK1, and p-Akt with the hypothesis that PN overexpression may upregulate the expressions of ILK1 and p-Akt. Moreover, we verified that the expressions of ILK1 and p-Akt were inhibited by the PN siRNA.…”

Section: Discussionmentioning

confidence: 99%

Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway

Zheng

Zhang

et al. 2015

Reprod. Sci.

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Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), and phospho-Akt (p-Akt) in NSCs, EuSCs as well as EcSCs before and after receiving PN small-interfering RNA (siRNA). The protein and messenger RNA (mRNA) levels of PN were upregulated in EuSCs (P < .05; P = .2261 in proliferative phase and P = .3385 in secretory phase) and EcSCs (P < .001; P < .001 in proliferative phase and P < .05 in secretory phase) compared with NSCs, although there was no significant difference in PN mRNA between EuSCs and NSCs. In EcSCs, abilities of migration, invasion, and adhesion and the expressions of ILK1 and p-Akt were enhanced; and all of those were downregulated after PN siRNA interference. Thus, PN enhanced ESCs migration, invasion, and adhesion due to the ILK1/Akt signal pathway. As an agonist in the development and progression of endometriosis, PN may be a new clinical treatment target of endometriosis.

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“…pathway was activated in endometriosis. Especially, p-AKT was highly expressed in endometriotic stromal cells, leading to the reduced decidualization and cell survival (26,(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

Systematic analysis of hsa-miR-363 gene overexpression pattern in endometrial stromal cells

Fan

Zhang

et al. 2018

Int J Mol Med

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Endometriosis is a benign disease, but has invasion and metastasis characteristics similar to malignant tumors. Clinically, it is a difficult problem of gynecological clinical treatment for its high recurrence rate. It has been confirmed that miR-363 was downregulated in endometriosis tissues and miR-363 overexpression inhibited the invasion ability of endometrial stromal cells (ESCs). In order to explore the potential mechanism of miR-363-reduced ESC migration and invasion progression, we sought to demonstrate the targeted mRNA expression levels of miR-363 through microarray, and performed cluster analysis to identify potential functions of these targeted genes in ESCs. The wound migration assay showed that there was an observable trend of cell migration potential decrease after transfection with hsa-miR-363. The qRT-PCR result showed that compared to miR-363 negative control cell group, miR-363 was upregulated 3,264.58-fold after miR-363 lentiviral transfection in miR-363 mimics group. The microarray data showed that compared to ESCs miR-363 negative control cell group, 249 genes were upregulated in ESCs miR-363 mimics cells group, and 139 genes were downregulated. Gene Ontology analysis and the pathway analysis data demonstrated that these target genes are mainly involved in cell migration, cell adhesion and invasion, proliferation, apoptosis, alteration of endometrial cells and some related signaling pathways. Our study explored the gene expression pattern after miR-363 overexpression, which could expand the insights into the miR-363 function and molecular mechanisms in endometriosis.

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Unremitting Cell Proliferation in the Secretory Phase of Eutopic Endometriosis: Involvement of pAkt and pGSK3β

Abstract: Unceasing cell proliferation in the secretory phase of eutopic endometriosis is linked to deregulation of c-kit/SCF-associated signaling pathways.

Cited by 17 publications

References 57 publications

Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid‑secretory phases

Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid‑secretory phases

Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway

Systematic analysis of hsa-miR-363 gene overexpression pattern in endometrial stromal cells

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