Systematic analysis of hsa-miR-363 gene overexpression pattern in endometrial stromal cells

Li, Wenqu; Fan, Xuemei; Zhang, Mi; Huang, Lei; Lv, Shanshan; Wang, Luyu; Wu, Ying; Dai, Chencheng; Xu, Juan; Xu, Pengfei; Fu, Ziyi; Jia, Xuemei; Shi, Xiaoyan

doi:10.3892/ijmm.2018.3840

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…[ 9 , 10 ]. The Matrigel was thawed at 4°C and diluted with serum-free DMEM at a ratio of 1 : 8; then, the mixed solution was added into the upper Transwell chamber at 60 μ l medium/well to incubate for 4 h under 37°C and 5% CO 2 conditions.…”

Section: Methodsmentioning

confidence: 99%

“…MicroRNA-363 (miR-363) is one of the miRNAs screened to be downregulated in papillary thyroid cancer [ 9 ]. Previous experiments prove that the miR-363 overexpression inhibited the invasion ability of endometrial stromal cells [ 10 ], proving that the abnormal expression of miR-363 may be of important significance in the pathogenesis of EMs. Some research verifies that the miR-363 expression is downregulated in multiple malignant tumors, which significantly promotes tumor cell growth and proliferation [ 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Nai

Zhang

et al. 2022

BioMed Research International

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EMs is a kind of benign disease with certain malignant behaviors. The adhesion, invasive growth, and angiogenesis of ectopic endometrial cells are the pathological basis of EMs occurrence, but its etiology and pathogenesis have not been completely illustrated yet. In our research, we aim to investigate the role of miR-363 in the pathogenesis of endometriosis. Real-time quantitative PCR was used to detect the expression of miR-363 before and after ESC/NSC transfection. CCK-8, flow cytometry, and transwell assay were used to detect the effect of the miR-363 expression on cell proliferation, apoptosis, and invasion. The effects of the miR-363 expression on the contents of Fas/APO-1 and ICAM-1 in cell culture supernatant were detected by ELISA. qRT-PCR and WB assay were used to detect the effects of the miR-363 expression on the mRNA and protein expression levels of ICAM-1, MMP-7, and VEGF in ESC. The increased expression of miR-363 could inhibit the proliferation and invasion of ESC, promote apoptosis, and inhibit the secretion of FAS/APO-1 and ICAM-1. The knockdown expression of miR-363 promoted proliferation and invasion of NSC, inhibited apoptosis, and promoted secretion of FAS/APO-1 and ICAM-1. VCAM-1, VEGF, and MMP-7 were detected in ESCs before transfection. The protein expression level was higher than that of NSCs. Compared with pretransfection, the protein levels of VCAM-1, VEGF, and MMP-7 in the M-363 group were significantly downregulated. The downregulated expression of miR-363 was associated with a stronger cell proliferation ability, a lower cell apoptosis rate, and a stronger ESC. Migration is associated with invasiveness, proliferation, angiogenesis, and immune escape. The low expression of miR-363 promotes endogenesis through posttranscriptional regulation of target genes VCAM-1, MMP-7, and VEGF. The differential expression of miR-363 between ESC and NSC may be an important factor in the many biological differences between ESC and NSC.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Nai

Zhang

et al. 2022

BioMed Research International

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“…These miRNAs were chosen as currently these miRNAs have not been identified as having differential expression in endometriosis, so were most appropriate for normalisation of data. However, it is important to note that these miRNAs may regulate endometrial cell processes important in endometriosis pathogenesis such as cell proliferation, adhesion and invasion (Li et al., 2018 ; Pan et al., 2017 ). For future research investigating EV miRNAs in cervical brushes or vaginal swabs, more in‐depth RNA analysis such as sequencing in a larger cohort of case and control samples could help to identify miRNAs that have stable abundance within cervico‐vaginal fluid EVs for normalisation strategies.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cervicovaginal fluid extracellular vesicles isolated from paired cervical brushes and vaginal swabs

Paterson,

Scheck,

McDowell

et al. 2024

J of Extracellular Bio

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Endometriosis is a common gynaecological condition, with a long diagnostic delay. Surgery is required to confirm a diagnosis, highlighting the need for a non‐invasive biomarker. Extracellular vesicles (EVs) may have a role in endometriosis pathogenesis, yet there is limited EV biomarker literature available. This study aimed to investigate the feasibility of isolating cervico‐vaginal fluid EVs sampled using cervical brushes and vaginal swabs and to compare these methods. After providing informed consent, patients undergoing surgery for suspected endometriosis had cervical brush and vaginal swab samples collected under general anaesthetic. Isolated EVs were characterised through negative stain transmission electron microscopy (TEM), Western blotting (TSG101, CD63, Calnexin, ApoB, Albumin), tunable resistive pulse sensing (TRPS), microBCA assays and RT‐qPCR of miRNAs. PCR was performed on samples prior to EV isolation to assess bacteria present in samples. Cervical brush and vaginal swab EVs were intact vesicles with limited co‐isolated contaminants. Cervical brushes had higher concentrations of particles compared to match vaginal swabs, although both samples had low concentrations. Protein and miRNA yield were similar between matched samples. PCR demonstrated only a small amount DNA within samples was bacterial (>0.5%). Cervico‐vaginal fluids EVs were successfully isolated from cervical brushes and vaginal swabs, demonstrating a new method of sampling reproductive EVs. EV yield from both sample types was low. Similar protein and miRNA levels suggest either sampling method may be suitable for biomarker studies.

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“…Treatment and biomarker [102] miR-196b -Affecting BCL-2 expression, activation of caspase-3 and caspase-7 Treatment [103] miR-363 Down-regulated Inducing apoptosis Treatment and biomarker [104] miR-21-5p -Affecting the apoptotic potential of stromal cells. Treatment [92] miR-9 Down-regulated Affecting BCL-2 expression Treatment and biomarker [83] miR181c -NR4A-miR181c-Mst1 pathway regulates mitochondrial apoptosis Treatment [105] miR-2861 Down-regulated Up-regulation of STAT3 and MMP2 and inducing apoptosis Treatment and biomarker [106] miR-141-3p -Down-regulation the expression of Bcl-2 and raising the expression of Bax and contributing to the apoptosis of ectopic ESCs Treatment [82] cell death; TNF: Tumor necrosis factor; MMP: Matrix metalloproteinase; ICE: Interleukin converting enzyme; DAPK1: Death-associated protein kinase 1.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic functions of microRNAs in pathogenesis, diagnosis, and treatment of endometriosis

et al. 2020

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MicroRNAs or miRNAs are a component of the non-coding RNAs family which is engaged in many cellular functions such as cell proliferation, apoptosis, gene expression, signaling pathways, angiogenesis, and etc. Endometriosis is a malignant gynecologic disorder occurring in women before menopausal age. Pathogenesis of this illness is still a discussion subject between the scientists but in our knowledge, microRNAs can be one of the possible involved factors. The purpose of this paper is to investigate the role of apoptotic activities of miRNAs in endometriosis. Accumulative evidence has demonstrated the role of cell proliferation, apoptosis, and invasion in the progression of these diseases. In this review, we looked into the specific role of apoptosis and its related genes and pathways in endometriosis and tied to present an explanation of how miRNAs can affect endometriosis by their apoptotic activities. What we found is that a great extent of miRNAs is involved in this illness and they are responsible for repressing apoptosis and progression of the disease. As a result, miRNAs have two different usages in endometriosis: biomarkers and potential therapeutic targets. In this review we gathered a great amount of evidence to inquire into the role of micro RNAs in inducing apoptosis and how this mechanism can be exerted for therapeutic purposes for endometriosis.

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Systematic analysis of hsa-miR-363 gene overexpression pattern in endometrial stromal cells

Cited by 4 publications

References 42 publications

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Comparison of cervicovaginal fluid extracellular vesicles isolated from paired cervical brushes and vaginal swabs

Apoptotic functions of microRNAs in pathogenesis, diagnosis, and treatment of endometriosis

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