Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

Baral, Jit B.; Bosland, Paul W.

doi:10.21273/jashs.129.6.0826

Cited by 45 publications

(46 citation statements)

References 16 publications

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“…is very popular in Mexico and the Caribbean region, with Habanero and Scotch Bonnet being the most representative varietal types. These three species, characterised by white flowers and yellow smooth seeds, are phylogenetically close and make up the annuum‐chinense‐frutescens complex 1, 5, 6…”

Section: Introductionmentioning

confidence: 99%

Volatile and capsaicinoid composition of ají (Capsicum baccatum) and rocoto (Capsicum pubescens), two Andean species of chile peppers

Kollmannsberger

Rodríguez‐Burruezo

Nitz

et al. 2011

J. Sci. Food Agric.

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Section: Introductionmentioning

confidence: 99%

Volatile and capsaicinoid composition of ají (Capsicum baccatum) and rocoto (Capsicum pubescens), two Andean species of chile peppers

Kollmannsberger

Rodríguez‐Burruezo

Nitz

et al. 2011

J. Sci. Food Agric.

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“…Furthermore, there is limited number of morphological descriptors in accession assignments (Rodriguez et al 1999;Bosland and Votava 2000;Jarret and Dang 2004;Baral and Bosland 2004). Characterization of accessions or species is an important step for the germplasm conservation, maintenance and breeding studies.…”

Section: Introductionmentioning

confidence: 99%

Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions

İnce

Karaca

Onus

2008

Genet Resour Crop Evol

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A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum. A touch down PCR profile and unique chemical concentration of ingredients resulted in reproducible and reliable DNA amplifications. The number of amplified products varied from 1 to 12 fragments depending on the template DNA and the primers. The DAMD-PCR technique provided a total of 38 accession specific DNA markers (diagnostic DAMD-PCR) which can be utilized in accession identification, preservation and genetic studies of Capsicum germplasm. Based on 1,292 polymorphic and monomorphic DNA markers directed with 22 minisatellite specific primers, accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Capsicum chacoense was found to be the most distinct species.

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“…[26,3,2] Most accessions belong to only one cluster, with the median coefficient of membership to the best-matching cluster being 0.99 (mean ±sd is 0.9471 ±0.13, Supplementary Figure S4). Nevertheless, some of the accessions seem misplaced probably because of mislabeling or misclassification [31] by germplasm providers. Like PCA, admixture clustering is not related to geography (Supplementary Figure S5).…”

Section: Resultsmentioning

confidence: 99%

Genomic diversity and novel genome-wide association with fruit morphology in Capsicum, from 746k polymorphic sites

Colonna

D’Agostino²,

Garrison

et al. 2018

Preprint

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Background: Capsicum is one of the major vegetable crops grown world-wide. Current subdivision in clades and species is based on morphological traits and coarse sets of genetic markers. Fruits broad variability has been driven by breeding programs and has been mainly studied by linkage analysis. Results:We discovered 746k variable sites by sequencing 1.8% of the genome in a collection of 373 accessions belonging to 11 Capsicum species from 51 countries. We describe genomic variation at population-level, confirm major subdivision in clades and species, and show that the known subdivision of C. annuum in two groups separates large and bulky fruits form small ones. In C. annuum, we identify four novel loci associated with phenotypes determining the fruit shape, including a non-synonymous mutation in the gene Longifolia 1-like (CA03g16080). Conclusions:Our collection covers all the economically important species of Capsicum widely used in breeding programs, and represent the widest and largest study so far in terms of the number of species and genetic variants analyzed. We identified a large set of markers that can be used for population genetic studies and genetic association analyses. Our results foster fine genetic association studies and foresee genomic variability at population-level.

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Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

Cited by 45 publications

References 16 publications

Volatile and capsaicinoid composition of ají (Capsicum baccatum) and rocoto (Capsicum pubescens), two Andean species of chile peppers

Volatile and capsaicinoid composition of ají (Capsicum baccatum) and rocoto (Capsicum pubescens), two Andean species of chile peppers

Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions

Genomic diversity and novel genome-wide association with fruit morphology in Capsicum, from 746k polymorphic sites

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