Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions

Abstract

A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum. A touch down PCR profile and unique chemical concentration of ingredients resulted in reproducible and reliable DNA amplifications. The number of amplified products varied from 1 to 12 fragments depending on the template DNA and the primers. The DAMD-PCR technique provided a total of 38 accession specific DNA markers (diagnostic DAMD-PCR) which can be utilized in accession identification, preservation and genetic studies of Capsicum germplasm. Based on 1,292 polymorphic and monomorphic DNA markers directed with 22 minisatellite specific primers, accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Capsicum chacoense was found to be the most distinct species.

Efficacy of Morphological Characters for Varietal Identification of Chilli

“…The leaf width was found to be short (3 cm) in three genotypes viz., CO1, CA97, Sln1 and CCH1 and medium (3-5 cm) for CO2, K1, K2, PLR1, PKM1, PMK1, KKM1 and CCH1. Eevera (2003) (Moscone et al, 2007 andInce et al, 2009). In chilli genotypes, calyx annular constriction was absent in K1, PKM1 and KKM1 while it was present in CO2, K2, PLR1, PMK1, CCH1, CA 97 and Sln1.…”

Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down-stream processes

“…DNA obtained using this mtDNA extraction method was relatively free of contaminants and was of sufficiently high-quality for other downstream processes. Ince et al (2009) andTurci et al (2011) reported that good DNA extraction methods should yield adequate, intact, and pure DNA in a short period of time at low cost and should be suitable for a large number of plant species.…”

Comparative assessment of ISSR, DAMD and SCoT markers for evaluation of genetic diversity and conservation of landrace chickpea (Cicer arietinum L.) genotypes collected from north-west of Iran

“…Based on the independent replications of DAMD, we observed that reproducible DNA markers were amplified and also noted that all DAMD primers used in this study produced RAPD like results but the numbers of bands were sharp and clear. The relatively high PCR stringencies in DAMD application effectively limited the PCR artifacts which commonly occur in RAPDs (Karaca et al 2002;Ince et al 2009). It was evident from the results that the dendrogram based on Fig.…”