Unraveling the Spatial Distribution of Immunoglobulins, Enzymes, and Polyelectrolytes within Layer-by-Layer Self-Assembled Multilayers. Ellipsometric Studies

Ferreyra, Nancy F.; Forzani, Erica; Teijelo, M. López; Coche-Guérente, Liliane; Labbé, Pierre

doi:10.1021/la061173q

Cited by 3 publications

(3 citation statements)

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“…Ferreyra, Teijelo, and coworkers prepared bioelectrodes composed of LbL films from an anionic alkaline phosphatase (AP)-polyphenol oxidase (PPO) mixture and the polycation PDDA on top of a glassy carbon electrode modified with an immunological layer. 269 They analyzed structural data obtained from these complex structures, including film thickness, the film optical properties, and the ellipsometric mass values assessed from the two models, threelayer film and reorganization layer. Combination of several analytical methods is sometimes necessary to characterize biological LbL films.…”

Section: Advanced Analyses and Novel Propertiesmentioning

confidence: 99%

Layer-by-layer assembly as a versatile bottom-up nanofabrication technique for exploratory research and realistic application

Ariga

Hill

2007

Phys. Chem. Chem. Phys.

1,168

865

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The layer-by-layer (LbL) adsorption technique offers an easy and inexpensive process for multilayer formation and allows a variety of materials to be incorporated within the film structures. Therefore, the LbL assembly method can be regarded as a versatile bottom-up nanofabrication technique. Research fields concerned with LbL assembly have developed rapidly but some important physicochemical aspects remain uninvestigated. In this review, we will introduce several examples from physicochemical investigations regarding the basics of this method to advanced research aimed at practical applications. These are selected mostly from recent reports and should stimulate many physical chemists and chemical physicists in the further development of LbL assembly. In order to further understand the mechanism of the LbL assembly process, theoretical work, including thermodynamics calculations, has been conducted. Additionally, the use of molecular dynamics simulation has been proposed. Recently, many kinds of physicochemical molecular interactions, including hydrogen bonding, charge transfer interactions, and stereo-complex formation, have been used. The combination of the LbL method with other fabrication techniques such as spin-coating, spraying, and photolithography has also been extensively researched. These improvements have enabled preparation of LbL films composed of various materials contained in well-designed nanostructures. The resulting structures can be used to investigate basic physicochemical phenomena where relative distances between interacting groups is of great importance. Similarly, LbL structures prepared by such advanced techniques are used widely for development of functional systems for physical applications from photovoltaic devices and field effect transistors to biochemical applications including nano-sized reactors and drug delivery systems.

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Section: Advanced Analyses and Novel Propertiesmentioning

confidence: 99%

Layer-by-layer assembly as a versatile bottom-up nanofabrication technique for exploratory research and realistic application

Ariga

Hill

2007

Phys. Chem. Chem. Phys.

1,168

865

View full text Add to dashboard Cite

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“…Ellipsometry is a method widely used to study the characteristics of thin films onto modified surfaces [23][24][25]. This technique measures the change in polarization state of light reflected from the surface of a sample in terms of the ellipsometric angles and [26].…”

Section: Fundamentals Of Ellipsometrymentioning

confidence: 99%

“…From the fitting procedure, the complex refractive index, written in terms of its real part, n (refractive index), and imaginary part, k (extinction coefficient), as ň = n -i k [26] were obtained. The optical constants of parallel (extraordinary) and perpendicular (ordinary) direction, referred as n or , k or , n ext and k ext , as well as the thickness for each experimental point, were determined [23].…”

Section: Fundamentals Of Ellipsometrymentioning

confidence: 99%