Surface Sensitization Techniques and Recognition Receptors Immobilization on Biosensors and Microarrays

Patterned Biochemical Functionalization Improves Aptamer-Based Detection of Unlabeled Thrombin in a Sandwich Assay

“…As APTES is a short molecule, layer thickness is thin and self-assembled monolayer (SAM) formation ability is restricted compared to SAMs originating from molecules with longer chains. 19 The chemical functionalization steps of planar glass surfaces prepared for further nanoplotting experiments were analyzed using atomic force microscopy (AFM), ellipsometry, contact angle measurements. Zeta-potential was measured with functionalized silicon nanowires in solution and APTES binding could be confirmed with Fourier transform infrared spectroscopy with attenuated total reflectance (ATR-FTIR).…”

“…18,19 High specificity of the whole biochemical scheme determines the detection limit of the technique. In parallel to already well-studied antibodies, 18,20 the use of engineered receptor molecules, which are more stable and can be developed at low cost, i.e., aptamers, still need to be optimized for their wider implementation into biochemical assays.…”

Carbonate hydroxyapatite functionalization: a comparative study towards (bio)molecules fixation

“…Theoretically, surface functionalization with APTES should afford a homogeneous coupling between surface OH groups and silanols from hydrolysed APTES ( figure 1a). However, the experimental result is a mixture of Si-O bond formation between the surface OH and hydrolysed APTES accompanied by physisorption that can also involve molecular inversion of APTES molecules ( figure 1b and c, respectively) [36]. Hydrogen bonding between surface OH and silanols/amino groups, though weak interactions, are competing with the condensation reaction; these physisorption processes hamper/slow down the condensation reaction, which in some cases can be ensured by drying procedures [37].…”

New concepts of integrated photonic biosensors based on porous silicon

“…Multifunctional silane is usually preferred due to its higher reactivity and because it can lead to lower non-specific binding. Silanization with aminopropyl triethoxy silane or 3-glycidopropyl trimethoxy silane is well documented in the literature (Dugas et al, 2010a). With multifunctional silane, additional intermolecular dehydration reactions between adjacent organo silanols lead to a 2D network.…”