Unraveling the proteomic changes involved in the resistance response of Cajanus platycarpus to herbivory by Helicoverpa armigera

Rathinam, Maniraj; Roschitzki, Bernd; Grossmann, Jonas; Mishra, Pragya; Kunz, Laura; Wolski, Witold; Panse, Christian; Tyagi, Shaily; Rao, Uma; Schlapbach, Ralph; Sreevathsa, Rohini

doi:10.1007/s00253-020-10787-6

Cited by 12 publications

(11 citation statements)

References 43 publications

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“…Insect pests have always been a menace in agriculture as their infestation results in enormous crop losses globally. In this regard, host plant resistance involving both morphological and biochemical traits has been found to be effective at minimizing damage from pests such as the notorious H. armigera [ 21 , 31 , 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…Based on our previous findings, [21,33] a total of 8 genes and their isoforms as listed previously were selected from the transcriptome data to assess their dynamic expression. Since we had earlier assessed the dynamic response of C4H and F3H (Figure 1A) [33], in the comparative proteomic analysis of C. platycarpus and C. cajan during herbivory, we selected other pivotal genes of the pathway. qRT-PCR analysis demonstrated that higher expression (4-11-fold increase) was…”

Section: H Armigeramentioning

confidence: 99%

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Deciphering of Pod Borer [Helicoverpa armigera (Hübner)] Resistance in Cajanus platycarpus (Benth.) Offers Novel Insights on the Reprogramming and Role of Flavonoid Biosynthesis Pathway

Tyagi

Shashank

Chaudhary

et al. 2022

Toxins

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Management of pod borer, Helicoverpa armigera in pigeonpea (Cajanus cajan L.), an important legume crop, has been a pertinent endeavor globally. As with other crops, wild relatives of pigeonpea are bestowed with various resistance traits that include the ability to deter the H. armigera. Understanding the molecular basis of pod borer resistance could provide useful leads for the management of this notorious herbivore. Earlier studies by our group in deciphering the resistance response to herbivory through multiomics approaches in the pigeonpea wild relative, Cajanus platycarpus, divulged the involvement of the flavonoid biosynthesis pathway, speculating an active chemical response of the wild relative to herbivory. The present study is a deeper understanding of the chemical basis of pod borer (H. armigera) resistance in, C. platycarpus, with focus on the flavonoid biosynthesis pathway. To substantiate, quantification of transcripts in H. armigera-challenged C. platycarpus (8 h, 24 h, 48 h, 96 h) showed dynamic upregulation (up to 11-fold) of pivotal pathway genes such as chalcone synthase, dihydroflavonol-4-reductase, flavonoid-3′5′-hydroxylase, flavonol synthase, leucoanthocyanidin reductase, and anthocyanidin synthase. Targeted LC-MS analyses demonstrated a concomitant increase (up to 4-fold) in naringenin, kaempferol, quercetin, delphinidin, cyanidin, epigallocatechin, and epicatechin-3-gallate. Interestingly, H. armigera diet overlaid with the over-produced flavonoids (100 ppm) showed deleterious effects on growth leading to a prolonged larval period demonstrating noteworthy coherence between over-accumulation of pathway transcripts/metabolites. The study depicts novel evidence for the directed metabolic reprogramming of the flavonoid biosynthesis pathway in the wild relative to pod borer; plant metabolic potential is worth exploiting for pest management.

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Section: Resultsmentioning

confidence: 99%

Section: H Armigeramentioning

confidence: 99%

Deciphering of Pod Borer [Helicoverpa armigera (Hübner)] Resistance in Cajanus platycarpus (Benth.) Offers Novel Insights on the Reprogramming and Role of Flavonoid Biosynthesis Pathway

Tyagi

Shashank

Chaudhary

et al. 2022

Toxins

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“…This study is also consistent with the transcriptomic studies of Njaci et al [ 19 ], where there was greater induction of CHS, an important flavonoid enzyme, in the wild, tolerant C. scarabaeoides accession in response to H. armigera infestation than in the susceptible variety, ICPL 87. Rathinam et al [ 39 ], reported a similar observation in the wild pigeonpea, C. platycarpus , with accession having more proteins involved in the phenylpropanoid pathway and flavonoid biosynthesis when compared to a cultivated pigeonpea genotype. Plants appear to reinforce defence against any tissue damage arising either from injuries induced by insects/pathogens or mechanical injuries by reallocating resources and producing secondary metabolites derived from the phenylpropanoid pathway [ 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 69%

Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

Ngugi-Dawit

Njaci

Higgins

et al. 2021

IJMS

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Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

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“…Protease inhibitors were reported to interfere with gut proteinases of H. armigera , leading to inactivation of the insect digestive system followed by insect mortality (Swathi et al, 2015). Subsequently, high expression of the genes associated with α‐linolenic acid metabolism was reported in C. platycarpus (Ramkumar et al, 2020; Rathinam et al, 2020) with an increase in time of pod borer infestation. Simultaneously incremental levels of lipoxygenase enzyme in Fusarium udum resistant accessions of pigeonpea were reported in an experiment employing mechanical wounding and inoculation of α‐linolenic acid, providing further evidence of the involvement of jasmonic acid in response to biotic stress (Uma Maheswari Devi, Srinivas Reddy, Usha Rani, Reddy, Narsa Reddy, & Reddanna, 2000).…”

Section: Discussionmentioning

confidence: 99%

Secondary genepool of Australian Cajanus species contains sources of resistance to Helicoverpa armigera (Hübner)

Vanambathina

Henry

Rachaputi

et al. 2021

Annals of Applied Biology

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A narrow genetic base in cultivated pigeonpea (Cajanus cajan (L.) Millsp.) limits the host plant resistance (HPR) to the devastating pest Helicoverpa armigera (Hübner). Earlier research on pod borer resistance in wild Cajanus relatives has mainly explored the tertiary genepool, making breeding for resistance difficult because of linkage drag. Previous investigations on a wide range of Australian native germplasm from the secondary and tertiary gene pools revealed resistance in the secondary gene pool, associated with total phenolic content (TPC). This study reports the application of the extreme phenotype genome‐wide association (XP‐GWAS) to associate single nucleotide polymorphisms (SNPs) with resistance traits. Mapping of 19 accessions, including 12 wild accessions to a reference pigeonpea genome have identified a total of 16,489 and 16,227 nonsynonymous SNPs for the resistance and TPC traits, respectively. These SNPs were subjected to functional analysis, and KEGG pathway analysis revealed the presence of candidate genes coding for the crucial enzymes in five defence‐related pathways that could be contributing to resistance. These pathways involve ‘Jasmonic acid synthesis’ (palmitoyl‐CoA hydrolase, phospholipase A1 and phospholipase A2), ‘insect hormone biosynthesis’ (aldehyde dehydrogenase) ‘Steroid biosynthesis’ (squalene monooxygenase, farnesyl transferase and lanosterol synthase), ‘Cutin, suberine and wax synthesis’ (O‐acyltransferase) and ‘Brassinosteroid biosynthesis’ (Det2). Analysis of SNPs associated with the TPC trait led to the identification of a divergent pathway of ‘secondary metabolite synthesis’ (flavonoid 3′ hydroxylase, l‐rhamnosyltransferase) in wild Cajanus species consistent with a high concentration of isoorientin and the possible presence of its derivative maysin; a strong insecticidal compound in leaves.

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Unraveling the proteomic changes involved in the resistance response of Cajanus platycarpus to herbivory by Helicoverpa armigera

Cited by 12 publications

References 43 publications

Deciphering of Pod Borer [Helicoverpa armigera (Hübner)] Resistance in Cajanus platycarpus (Benth.) Offers Novel Insights on the Reprogramming and Role of Flavonoid Biosynthesis Pathway

Deciphering of Pod Borer [Helicoverpa armigera (Hübner)] Resistance in Cajanus platycarpus (Benth.) Offers Novel Insights on the Reprogramming and Role of Flavonoid Biosynthesis Pathway

Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

Secondary genepool of Australian Cajanus species contains sources of resistance to Helicoverpa armigera (Hübner)

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