Unraveling the camel rumen microbiome through metaculturomics approach for agriculture waste hydrolytic potential

Srivastava, Shweta; Dafale, Nishant A.; Jakhesara, Subhash J.; Joshi, Chaitanya; Patil, N. B.; Purohit, Hemant J.

doi:10.1007/s00203-020-02010-x

Cited by 18 publications

(5 citation statements)

References 31 publications

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“…Cellulase and xylanase production is inducible as it depends on various factors such as inoculum size, pH value, temperature, medium additives, fermentation time, and growth substrate [12,23,26,28,63]. Xylanase and cellulase productions were in the range indicated by similar studies [64][65][66] for rumen bacteria. On the other hand, the anaerobic bacterial community in this study produced more xylanase and cellulase than the aerobic fungi [67] and anaerobic rumen fungi of the camel gut [26].…”

Section: Enzymes Productionmentioning

confidence: 85%

Effect of barley straw and Egyptian clover hay on the rumen fermentation and structure and fibrolytic activities of rumen bacteria in dromedary camel

Rabee

2022

Vet World

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Background and Aim: Understanding the regulations of rumen microbiota and their fibrolytic capabilities under different forages are essential to improve rumen fermentation and animal feed efficiency. This study aimed to evaluate the changes in the rumen fermentation and the structure and fibrolytic activities of rumen bacteria in camels fed barley straw and Egyptian clover hay. Materials and Methods: Three fistulated camels were fed a diet containing barley straw for 30 days; then transitioned to a diet containing Egyptian clover hay for 30 days. In addition, bacterial media enriched with xylan and different cellulose sources, namely, filter paper, wheat straw, and alfalfa hay, were used to evaluate the ability of camel rumen bacteria to produce xylanase and cellulase enzymes. Results: The camel group fed Egyptian clover hay showed higher crude protein intake, rumen ammonia, total volatile fatty acids, and acetic acid. Moreover, the camel group fed barley straw showed higher neutral detergent fiber intake, rumen pH, and propionic and butyric acids. Principal component analysis showed that bacterial communities were separated based on the forage type. Forage type affected the composition of rumen bacteria and most of the bacterial community was assigned to phylum Bacteroidetes and Firmicutes. Egyptian clover hay diet increased the proportions of genus Prevotella and Ruminococcus; while fed barley straw diet increased the Butyrivibrio, RC9_gut_group, and Fibrobacteres. The bacterial culture of the Egyptian clover hay fed group produced the greatest xylanase and the bacterial culture of the barley straw fed group produced the maximum cellulase. Conclusion: Egyptian clover hay is recommended to feed camels in intensive production. Moreover, the bacterial community in the camel rumen is a promising source of lignocellulolytic enzymes.

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Section: Enzymes Productionmentioning

confidence: 85%

Effect of barley straw and Egyptian clover hay on the rumen fermentation and structure and fibrolytic activities of rumen bacteria in dromedary camel

Rabee

2022

Vet World

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“…Thus, understanding of the enzymology of plant cell wall hydrolysis is the key step to the sustainable utilization of cellulosic biomass [36]. Cellulase and xylanase production by rumen inocula (Table 1) from camel and sheep were higher than the production of bacterial cultures isolated from camel rumen by Srivastava et al (2021) [50]. Moreover, cellulase production was similar to the production of some commercial bacterial strains such as E. coli [18] and cellulolytic bacterial consortium [22], which highlight the higher cellulolytic activities of rumen bacteria in sheep and camel.…”

Section: Hydrolysis Of Barely Straw Ethanol and Gas Productionmentioning

confidence: 99%

Fibrolytic rumen bacteria of camel and sheep and their applications in the bioconversion of barley straw to soluble sugars for biofuel production

et al. 2022

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Lignocellulosic biomass such as barley straw is a renewable and sustainable alternative to traditional feeds and could be used as bioenergy sources; however, low hydrolysis rate reduces the fermentation efficiency. Understanding the degradation and colonization of barley straw by rumen bacteria is the key step to improve the utilization of barley straw in animal feeding or biofuel production. This study evaluated the hydrolysis of barley straw as a result of the inoculation by rumen fluid of camel and sheep. Ground barley straw was incubated anaerobically with rumen inocula from three fistulated camels (FC) and three fistulated sheep (FR) for a period of 72 h. The source of rumen inoculum did not affect the disappearance of dry matter (DMD), neutral detergent fiber (NDFD). Group FR showed higher production of glucose, xylose, and gas; while higher ethanol production was associated with cellulosic hydrolysates obtained from FC group. The diversity and structure of bacterial communities attached to barley straw was investigated by Illumina Mi-Seq sequencing of V4-V5 region of 16S rRNA genes. The bacterial community was dominated by phylum Firmicutes and Bacteroidetes. The dominant genera were RC9_gut_group, Ruminococcus, Saccharofermentans, Butyrivibrio, Succiniclasticum, Selenomonas, and Streptococcus, indicating the important role of these genera in lignocellulose fermentation in the rumen. Group FR showed higher RC9_gut_group and group FC revealed higher Ruminococcus, Saccharofermentans, and Butyrivibrio. Higher enzymes activities (cellulase and xylanase) were associated with group FC. Thus, bacterial communities in camel and sheep have a great potential to improve the utilization lignocellulosic material in animal feeding and the production of biofuel and enzymes.

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“…En un estudio reciente, realizado por Srivastava y col. [25] demostraron que, las bacterias celulolíticas y hemicelulolíticas del rumen de camello suelen ser excelentes bioprocesadoras celulósicas a partir de residuos agrícolas para la obtención de biocombustibles; y Mateo y col. [14] afirman, que en un cultivo de bacterias ruminales degradadoras de aserrín, un incremento en su degradación in vitro, del 21 y 59,7 %. Arcos y col. [2] encontraron a F. succinogenes aislados de bovinos en pastoreo con mayor capacidad degradativa de pared celular de Bouteloua repens (Navajita rastrera) que la cepa de referencia F. succinogenes ATCC 19169 (13,77 vs. 7,94 %).…”

Section: Bajounclassified

Bacterias fibrolíticas aisladas de rumen de alpaca, ovino y vacuno con capacidad biodegradadora de celulosa

et al. 2022

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Las bacterias ruminales celulolíticas se utilizan en la alimentación de rumiantes por su capacidad biodegradable de forrajes fibrosos. Sin embargo, existen escasos estudios en alpaca, ovino y vacuno si constituyen fuentes de microrganismos degradadores de celulosa para aplicaciones en biotecnología alimentaria. En esta investigación se aisló y evaluó el potencial degradador in vitro de la celulosa de las bacterias ruminales celulíticas de alpaca, ovino y vacuno. Se recolectaron muestras de líquido ruminal de ochos especímenes de alpaca, vacuno y ovino del matadero municipal de la localidad de Huancavelica – Perú, ubicado a 3820 metros sobre el nivel del mar en Perú. Las muestras ruminales fueron cultivadas en medios con carboximetilcelulosa, enriquecidos con caldo infusión cerebro corazón en condiciones aeróbicas y anaeróbicas hasta lograr desarrollo de colonias bacterianas. Luego se realizó la caracterización microbiológica, bioquímica y análisis de producción de celulasas de cada aislado bacteriano usando el método de coloración de rojo Congo y se evaluó el diámetro de los halos (mayor a 10-14 milímetros (mm)) de degradabilidad de celulosa. Se encontró bacterias celulolíticas Ruminococcus flavefaciens, Ruminococcus albus y Fibrobacter succinogenes con alta capacidad de degradabilidad de celulosa (halo mayor a 14 mm). Este hallazgo indica que los líquidos ruminales de alpaca, vacuno y ovino son excelentes fuentes de bacterias productoras de celulasas con alta capacidad degradadora de celulosa.

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Unraveling the camel rumen microbiome through metaculturomics approach for agriculture waste hydrolytic potential

Cited by 18 publications

References 31 publications

Effect of barley straw and Egyptian clover hay on the rumen fermentation and structure and fibrolytic activities of rumen bacteria in dromedary camel

Effect of barley straw and Egyptian clover hay on the rumen fermentation and structure and fibrolytic activities of rumen bacteria in dromedary camel

Fibrolytic rumen bacteria of camel and sheep and their applications in the bioconversion of barley straw to soluble sugars for biofuel production

Bacterias fibrolíticas aisladas de rumen de alpaca, ovino y vacuno con capacidad biodegradadora de celulosa

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