Unraveling the Biochemical and Molecular Networks Involved in Maize Cell Habituation to the Cellulose Biosynthesis Inhibitor Dichlobenil

Mélida, Hugo; Encina, Antonio; Álvarez, Jesús Miguel Muñoz; Acebes, José Luis; Caparrós-Ruiz, David

doi:10.1093/mp/ssq027

Cited by 25 publications

(56 citation statements)

References 67 publications

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“…This alcohol insoluble residue (AIR) was dried at 60ºC for 2 days. AIR was then de-starched, treated with acidified phenol and washed with organic solvents to obtain the cell wall residue as previously described [54,55].…”

Section: Cell Wall Analysismentioning

confidence: 99%

Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize

et al. 2015

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Coumarate 3-hydroxylase (C3H) catalyzes a key step of the synthesis of the two main lignin subunits, guaiacyl (G) and syringyl (S) in dicotyledonous species. As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knockdown maize plants. The results obtained indicate that C3H1 participates in lignin biosynthesis as its down-regulation redirects the phenylpropanoid flux: as a result, increased amounts of phydroxyphenyl (H) units, lignin-associated ferulates and the flavone tricin were detected in transgenic stems cell walls. Altogether, these changes make stem cell walls more degradable in the most C3H1-repressed plants, despite their unaltered polysaccharide content. The increase in H monomers is moderate compared to C3H deficient Arabidopsis and alfalfa plants. This could be due to the existence of a second maize C3H protein (C3H2) that can compensate the reduced levels of C3H1 in these C3H1-RNAi maize plants. The reduced expression of C3H1 alters the macroscopic phenotype of the plants, whose growth is inhibited proportionally to the extent of C3H1 repression. Finally, the down-regulation of C3H1 also increases the synthesis of flavonoids, leading to the accumulation of anthocyanins in transgenic leaves.

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Section: Cell Wall Analysismentioning

confidence: 99%

Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize

et al. 2015

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“…This is the case of bean cell cultures where habituation to dichlobenil is associated with high class III-per (García-Angulo et al, 2009). In the case of maize cells, an increased antioxidant capacity seems to take part in changes associated to the incipient dichlobenil-habituation process (Largo-Gosens et al, 2016), however, antioxidant activities are not implicated in the long-term habituation to high dichlobenil concentrations (Mélida et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

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The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells.

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“…By compositional analysis and structural characterization of the cell walls of DCB‐habituated cells, it has been demonstrated that maize cells have the capacity to cope with DCB through acquisition of a modified cell wall. In DCB‐habituated cells, cellulosic scaffold was partially replaced by a more extensive network of highly cross‐linked feruloylated arabinoxylans (Mélida et al , , , , , de Castro et al , , Largo‐Gosens et al ). Recently, it has been shown that DCB‐habituated maize cells incorporate to the cell walls lignin‐like polymers (Mélida et al ), which stiffens the structure further (Pomar et al , Kärkönen and Kuchitsu ).…”

Section: Introductionmentioning

confidence: 99%

Class III peroxidases in cellulose deficient cultured maize cells during cell wall remodeling

Martínez-Rubio

Acebes

Encina

et al. 2018

Physiologia Plantarum

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Maize (Zea mays L.) suspension-cultured cells habituated to a cellulose biosynthesis inhibitor 2,6-dichlorobenzonitrile (DCB) have a modified cell wall, in which the reduction in the cellulose content is compensated by a network of highly cross-linked feruloylated arabinoxylans and the deposition of lignin-like polymers. For both arabinoxylan cross-linking and lignin polymerization, class III peroxidases (POXs) have been demonstrated to have a prominent role. For the first time, a comparative study of POX activity and isoforms in control and cellulose-impaired cells has been addressed, also taking into account their cellular distribution in different compartments. Proteins from the spent medium (SM), soluble cellular (SC), ionically (ICW) and covalently bound cell wall protein fractions were assayed for total and specific peroxidase activity by using coniferyl and sinapyl alcohol and ferulic acid as substrates. The isoPOX profile was obtained by isoelectric focusing. POX activity was higher in DCB-habituated than in non-habituated cells in all protein fractions at all cell culture stages. For all substrates assayed, SC and ICW fractions showed higher activity at the early log growth phase than at the late log phase. However, the highest POX activity in the spent medium was found at the late log phase. According to the isoPOX profiles, the highest diversity of isoPOXs was detected in the ICW and SM protein fractions. The latter fraction contained isoPOXs with higher activity in DCB-habituated cells. Some of the isoPOXs detected could be involved in cross-linking of arabinoxylans and in the lignin-like polymer formation in DCB-habituated cells.

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Unraveling the Biochemical and Molecular Networks Involved in Maize Cell Habituation to the Cellulose Biosynthesis Inhibitor Dichlobenil

Cited by 25 publications

References 67 publications

Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize

Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Class III peroxidases in cellulose deficient cultured maize cells during cell wall remodeling

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