Unraveling Framework Nucleic Acid–Skin Cell Interactions with a Co‐Culture System

Wiraja, Christian; Siantoputri, Maria Esterlita; Liu, Sihan; Shum, Ho Cheung; Xu, Chen

doi:10.1002/adbi.201900169

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Wiraja et al developed an optically clear SE system to visualize the interaction between nucleic acid and skin cells. [79] The keratinocytes and dermal fibroblasts were cocultured in a microfluidic device to permit their crosstalks and facilitate enhanced barrier function. Given its optical clarity, the device allowed real-time visualization of the permeation process through fluorescence imaging, including the internalization kinetic and final localization of nanoparticles inside the skin cells.…”

Section: Sensor Development For Monitoring Of Skin Healthmentioning

confidence: 99%

Recent Progress in Skin‐on‐a‐Chip Platforms

Cui

Wiraja

Zheng

et al. 2021

Advanced Therapeutics

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As the largest organ of the body, the skin acts as a physical barrier for preventing excessive water loss and infection, and the host of many cosmetic and pharmaceutical products. Currently, two-dimensional (2D) cell culture and animal models are extensively used as mainstream platforms to understand skin biology and assess therapeutic drug efficacy. However, 2D cell culture model fails to recapitulate the intricate interactions between cells and the extracellular matrix. The metabolic state of 2D-cultured cells is typically similar, unlike the physiological condition in the body. Also, there is a growing ethical concern over the use of animal studies. Significant advances in microfluidics and tissue engineering have led to the development of three-dimensional (3D) skin-on-a-chip (SOC) technology, which offers a cost-effective alternative to conventional preclinical models for the validation and testing of pharmaceutical and cosmetic products. This review discusses the recent developments in the design of various SOCs, their fabrication strategies and methods for introducing skill cells to the chip, and their respective biomedical applications. Finally, an outlook on current applications, challenges, and future research direction in this field is provided.

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Section: Sensor Development For Monitoring Of Skin Healthmentioning

confidence: 99%

Recent Progress in Skin‐on‐a‐Chip Platforms

Cui

Wiraja

Zheng

et al. 2021

Advanced Therapeutics

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“…So far, a variety of TDD carriers or transdermal penetration enhancers have been developed for treatment of skin diseases. − However, those conventional TDD carriers or enhancers are mostly designed for delivery of small molecular drugs (e.g., with molecular weight below 600 Da) , and usually would be ineffective for macromolecular therapeutics. − Recently, DNA nanostructures formed by self-assembly of single-stranded DNA with good biocompatibility and controllable size/shape have also been explored for transdermal delivery applications. Among them, DNA tetrahedral structure with a side length of 21 base pairs (TH21) has been found to have excellent transdermal effect, − enabling transdermal delivery of macromolecule such as nucleic acids. , However, transdermal delivery of immunotherapy for treatment of skin cancer using DNA-based TDD systems has not yet been reported to our best knowledge.…”

Section: Introductionmentioning

confidence: 99%

Framework Nucleic Acid Immune Adjuvant for Transdermal Delivery Based Chemo-immunotherapy for Malignant Melanoma Treatment

et al. 2022

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Despite the tremendous progresses of cancer immunotherapy, its current clinical responses rate in melanoma remains to be improved. Here, we have reported a skin penetrating tetrahedral framework nucleic acid immune adjuvant (FNAIA) to transdermally deliver chemotherapy drugs into melanoma to induce the immunogenic death of tumor cells and expose tumor antigens, which with assistance of CpG oligodeoxynucleotide incorporated in FNAIA could trigger systemic tumor-specific immune responses. Compared with free CpG, FNAIA could penetrate deeper into subcutaneous tumor tissues and more effectively stimulate dendritic cell maturation. Notably, doxorubicin-loaded FNAIA locally applied on the intact skin above the melanoma could effectively inhibit the growth of mouse B16F10 melanoma and increase tumor CD8 + T cell infiltration. Moreover, combined with immune checkpoint inhibitor, the growth of distant tumors could also be effectively inhibited, suggesting that this strategy could induce systemic immune responses. Therefore, this work provides a new idea for non-invasive treatment of skin cancer.

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Research Progress on Bioactive Factors against Skin Aging

He,

Gao,

Guo

et al. 2024

IJMS

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The relentless pursuit of effective strategies against skin aging has led to significant interest in the role of bioactive factors, particularly secondary metabolites from natural sources. The purpose of this study is to meticulously explore and summarize the recent advancements in understanding and utilization of bioactive factors against skin aging, with a focus on their sources, mechanisms of action, and therapeutic potential. Skin, the largest organ of the body, directly interacts with the external environment, making it susceptible to aging influenced by factors such as UV radiation, pollution, and oxidative stress. Among various interventions, bioactive factors, including peptides, amino acids, and secondary metabolites, have shown promising anti-aging effects by modulating the biological pathways associated with skin integrity and youthfulness. This article provides a comprehensive overview of these bioactive compounds, emphasizing collagen peptides, antioxidants, and herbal extracts, and discusses their effectiveness in promoting collagen synthesis, enhancing skin barrier function, and mitigating the visible signs of aging. By presenting a synthesis of the current research, this study aims to highlight the therapeutic potential of these bioactive factors in developing innovative anti-aging skin care solutions, thereby contributing to the broader field of dermatological research and offering new perspectives for future studies. Our findings underscore the importance of the continued exploration of bioactive compounds for their potential to revolutionize anti-aging skin care and improve skin health and aesthetics.

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Unraveling Framework Nucleic Acid–Skin Cell Interactions with a Co‐Culture System

Cited by 3 publications

References 32 publications

Recent Progress in Skin‐on‐a‐Chip Platforms

Recent Progress in Skin‐on‐a‐Chip Platforms

Framework Nucleic Acid Immune Adjuvant for Transdermal Delivery Based Chemo-immunotherapy for Malignant Melanoma Treatment

Research Progress on Bioactive Factors against Skin Aging

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