Unraveling Cancer Metastatic Cascade Using Microfluidics-based Technologies

Hakim, Maziar; Kermanshah, Leyla; Abouali, Hesam; Hashemi, Hanieh Mohammad; Yari, Alireza; Khorasheh, Farhad; Alemzadeh, Iran; Vossoughi, Manouchehr

doi:10.1007/s12551-022-00944-8

Cited by 6 publications

(4 citation statements)

References 194 publications

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“…68 The mesenchymal phenotype acquired through EMT enables cancer cells to navigate through the ECM and breach the basement membrane, prerequisites for entering the bloodstream. 69,70 Once in the bloodstream, these cells can be transported to distant sites in the body, initiating the formation of metastatic lesions. Utilizing a device that assesses endothelial barrier permeability, the platform demonstrates soluble biochemical factors such as TNF-α in conjunction with the presence of macrophages to enhance the intravascular penetration of cancer cells, while also influencing the interaction between tumor and endothelial cells.…”

Section: Intravasationmentioning

confidence: 99%

Understanding organotropism in cancer metastasis using microphysiological systems

Ko,

Song,

Lee

et al. 2024

Lab Chip

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Section: Intravasationmentioning

confidence: 99%

Understanding organotropism in cancer metastasis using microphysiological systems

Ko,

Song,

Lee

et al. 2024

Lab Chip

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“…These activities are influenced by tumor cell interactions with interstitial flow and diffusion of morphogens to the nearby microvascular network. A wide review is made on the recent developments with microfluidics to understand the cancer microenvironemnt undergoing metastatic cascade with microscopy by Hakim et al, and Del Piccolo et al 143 , 144 .…”

Section: Tumour Barriermentioning

confidence: 99%

Models for barrier understanding in health and disease in lab-on-a-chips

et al. 2023

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The maintenance of body homeostasis relies heavily on physiological barriers. Dysfunction of these barriers can lead to various pathological processes, including increased exposure to toxic materials and microorganisms. Various methods exist to investigate barrier function in vivo and in vitro. To investigate barrier function in a highly reproducible manner, ethically, and high throughput, researchers have turned to non-animal techniques and micro-scale technologies. In this comprehensive review, the authors summarize the current applications of organ-on-a-chip microfluidic devices in the study of physiological barriers. The review covers the blood-brain barrier, ocular barriers, dermal barrier, respiratory barriers, intestinal, hepatobiliary, and renal/bladder barriers under both healthy and pathological conditions. The article then briefly presents placental/vaginal, and tumour/multi-organ barriers in organ-on-a-chip devices. Finally, the review discusses Computational Fluid Dynamics in microfluidic systems that integrate biological barriers. This article provides a concise yet informative overview of the current state-of-the-art in barrier studies using microfluidic devices.

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“…Microfluidic organ-on-a-chip-based cell culture devices are valuable tools that enable co-culturing of 3D cell culture systems in a spatially controlled manner (e.g., controlled perfusion flow and gradient control of cytokines) to better simulate tissue and organ physiologies [23]. Microfluidic technology, with its unique characteristics, has become a promising tool in cancer research, especially for studying the metastasis process [46]. The details of each step in the metastasis process are not yet fully understood, and appropriate models are, therefore, needed to shed light on the complex multistep process.…”

Section: Microfluidic Organ-on-a-chip-based Cell Culture Devicesmentioning

confidence: 99%

“…The details of each step in the metastasis process are not yet fully understood, and appropriate models are, therefore, needed to shed light on the complex multistep process. For example, mouse models have been previously exploited to study the mechanism of metastasis, but animal models have also been challenged as they possess some disadvantages [46]. By mimicking the TME and angiogenesis, microfluidic platforms are used to study in vitro tumor growth, and the platforms are utilized for the detection and identification of CTCs with different phenotypes [46].…”

Section: Microfluidic Organ-on-a-chip-based Cell Culture Devicesmentioning

confidence: 99%

Applications of Tumor Cells in an In Vitro 3D Environment

Hasterok,

Gustafsson,

Gjörloff Wingren

2023

Applied Sciences

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Spherical, multicellular aggregates of tumor cells, or three-dimensional (3D) tumor models, can be grown from established cell lines or dissociated cells from tissues in a serum-free medium containing appropriate growth factors. Air–liquid interfaces (ALIs) represent a 3D approach that mimics and supports the differentiation of respiratory tract and skin 3D models in vitro. Many 3D tumor cell models are cultured in conjunction with supporting cell types, such as fibroblasts, endothelial cells, or immune cells. To further mimic the in vivo situation, several extracellular matrix models are utilized to support tumor cell growth. Scaffolds used for 3D tumor cell culture growth include both natural and synthetic hydrogels. Three-dimensional cell culture experiments in vitro provide more accurate data on cell-to-cell interactions, tumor characteristics, drug discovery, metabolic profiling, stem cell research, and diseases. Moreover, 3D models are important for obtaining reliable precision data on therapeutic candidates in human clinical trials before predicting drug cytotoxicity. This review focuses on the recent literature on three different tissue types of 3D tumor models, i.e., tumors from a colorectal site, prostate, and skin. We will discuss the establishment of 3D tumor cell cultures in vitro and the requirement for additional growth support.

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Unraveling Cancer Metastatic Cascade Using Microfluidics-based Technologies

Cited by 6 publications

References 194 publications

Understanding organotropism in cancer metastasis using microphysiological systems

Understanding organotropism in cancer metastasis using microphysiological systems

Models for barrier understanding in health and disease in lab-on-a-chips

Applications of Tumor Cells in an In Vitro 3D Environment

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