Unraveling antibiotic resistance mechanisms in Mycobacterium abscessus: the potential role of efflux pumps

Steenwinkel, Jurriaan E M de; Schildkraut, Jodie A.; Ammerman, Nicole C.; Vogel, Corné P. de; Steenwinkel, Jurriaan E.M. de; Ingen, Jakko van; Bax, Hannelore I.

doi:10.1016/j.jgar.2022.10.015

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…Efflux pumps, which are membrane proteins necessary to maintain bacterial homeostasis by expelling toxic substances, are present in all types of bacteria [86]. Bacteria present in the biofilms also use efflux pumps to maintain cytoplasmic concentrations of certain antibacterial compounds below critical thresholds or to expel antibiotics, and the antibiotic resistance of the biofilm is caused by the increased expression or activity of the efflux pumps [87,88]. The ATP binding box (ABC) family, the small multi-drug resistant (SMR) family, the multi-drug and toxic compound extrusion (MATE) family, the drug-resistant nodular cell division (RND) family, and the large promoter superfamily (MFS) are the five major families of efflux pumps, and they play an important role in biofilm antibiotic resistance [89,90].…”

Section: Increased Expression Of Efflux Pumps In Biofilmsmentioning

confidence: 99%

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Yin

Cheng

et al. 2023

IJMS

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Bacterial biofilms can cause widespread infection. In addition to causing urinary tract infections and pulmonary infections in patients with cystic fibrosis, biofilms can help microorganisms adhere to the surfaces of various medical devices, causing biofilm-associated infections on the surfaces of biomaterials such as venous ducts, joint prostheses, mechanical heart valves, and catheters. Biofilms provide a protective barrier for bacteria and provide resistance to antimicrobial agents, which increases the morbidity and mortality of patients. This review summarizes biofilm formation processes and resistance mechanisms, as well as the main features of clinically persistent infections caused by biofilms. Considering the various infections caused by clinical medical devices, we introduce two main methods to prevent and treat biomaterial-related biofilm infection: antibacterial coatings and the surface modification of biomaterials. Antibacterial coatings depend on the covalent immobilization of antimicrobial agents on the coating surface and drug release to prevent and combat infection, while the surface modification of biomaterials affects the adhesion behavior of cells on the surfaces of implants and the subsequent biofilm formation process by altering the physical and chemical properties of the implant material surface. The advantages of each strategy in terms of their antibacterial effect, biocompatibility, limitations, and application prospects are analyzed, providing ideas and research directions for the development of novel biofilm infection strategies related to therapeutic materials.

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Section: Increased Expression Of Efflux Pumps In Biofilmsmentioning

confidence: 99%

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Yin

Cheng

et al. 2023

IJMS

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“…However, they did not appear to have altered MICs to hydrophobic or hydrophilic antibiotics ( 18 ). Mycobacterial efflux pumps have been associated with resistance to antibiotics such as bedaquiline, clofazemine, clarithromycin, amikacin, and tigecycline ( 19 – 22 ). Expression of efflux pump genes has been shown to be upregulated by antibiotic stress ( 22 ).…”

Section: Resultsmentioning

confidence: 99%

“…Mycobacterial efflux pumps have been associated with resistance to antibiotics such as bedaquiline, clofazemine, clarithromycin, amikacin, and tigecycline ( 19 – 22 ). Expression of efflux pump genes has been shown to be upregulated by antibiotic stress ( 22 ). It is unknown whether disabling MAB_4099c directly affects the regulation of efflux pump genes, yet it may be hypothesized that Tn_4099c may have increased cell wall permeability, allowing increased, albeit sublethal, exposure to the antibiotics, which may then lead to upregulation of efflux pump genes and thus paradoxical increased antibiotic resistance.…”

Section: Resultsmentioning

confidence: 99%

Glycopeptidolipid Defects Leading to Rough Morphotypes of Mycobacterium abscessus Do Not Confer Clinical Antibiotic Resistance

et al. 2023

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“…Furthermore, the presence of a class A β-lactamase leads to resistance against β-lactam antibiotics (Soroka et al 2014) and the enzymatic inactivation of tetracycline presents a hurdle in its effectiveness (Luthra et al 2018). Mounting evidence supports the involvement of efflux pumps in antibiotic resistance in M. abscessus (Mudde et al 2022). Efflux pumps play a significant role in drug resistance by actively removing toxic substances, including antibiotics, from the cell.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Strains within the Mycobacterium abscessus Complex and Determination of Resistance to Macrolides and Aminoglycosides

Kania,

Wόjcik,

Czekajewska

et al. 2023

Polish Journal of Microbiology

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One of the most relevant and pathogenic groups among the rapidly growing mycobacteria (RGM) is Mycobacterium abscessus complex (MABC) that includes three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense. The aim of this study was the analysis of prevalence of MABC among other non-tuberculous mycobacteria isolated from patients in the Malopolska Region of Poland, between 2018 and 2021, as well as determination of their subspecies and molecular mechanisms of resistance to macrolides and aminoglycosides. The incidence of MABC was 5,4% (12/223). Eight strains were classified as M. abscessus subsp. abscessus, three as M. abscessus subsp. massiliense and one M. abscessus subsp. bolletii. Molecular analysis showed resistance to macrolides for eight strains of M. abscessus subsp. abscessus associated with erm(41)T28 gene mutations. One strain of M. abscessus subsp. abscessus showed resistance to macrolides (two mutations simultaneously: in erm(41)T28 and rrl genes) and aminoglycosides (point mutation in rrs gene). One strain of M. abscessus subs. bolletii was resistant to macrolides (erm(41)T28 mutation), whereas presented no mutations for aminoglycosides. M. abscessus subsp. massiliense reveal no mutations. High clarithromycin resistance of M. abscessus, determines the urgent need for susceptibility-based treatment. Molecular determination of resistance mechanisms to aminoglycosides and macrolides enables fast and accurate targeted treatment implementation.

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Unraveling antibiotic resistance mechanisms in Mycobacterium abscessus: the potential role of efflux pumps

Cited by 13 publications

References 38 publications

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Glycopeptidolipid Defects Leading to Rough Morphotypes of Mycobacterium abscessus Do Not Confer Clinical Antibiotic Resistance

Molecular Identification of Strains within the Mycobacterium abscessus Complex and Determination of Resistance to Macrolides and Aminoglycosides

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