This paper focuses on the processes of translation in nanomedical research practices and contributes to a context- and object-centred research agenda in Science and Technology Studies. In particular, it addresses how nano-specific issues in medical research are exacerbated by uncertainty and unpredictability. Analyzing the relationship between nanomedicine and nanotoxicology I discuss how scientists are involved in highly uncertain processes, which require a contingent and experimental approach to nano-objects in everyday laboratory practices. Consequently, the dealings with nanomedical materials evoke a reformulation of numerous traditional forms of toxicological knowledge and knowledge practices, and challenge the self-concept of toxicology as a testing discipline.