UNIVmAb reactive albumin associated hyaladherin as a potential biomarker for colorectal cancer

Abstract

Colorectal cancer (CRC) is the third most common cancer; cancer biomarker discovery is important for disease detection and management. It is known that hyaluronic acid and its receptors are ubiquitously expressed in almost all human tissues. Earlier we have shown that a monoclonal antibody H11B2C2, presently known as UNIVmAb, reactive hyaladherin expressed in multiple human cancers mainly using immunohistochemistry. However, the nature of the antigen and its sequence homology are not known. In the current study, a comprehensive investigation was performed to explore the nature of the antigen and its homology using both biochemical and proteomic analysis. Our results showed that UNIVmAb reactive 57 kDa antigen was overexpressed in advanced grade colorectal cancer tissues compared to benign and its adjacent benign tissues. Biochemical investigations including biotinylated hyaluronic acid-pulldown, Immunoprecipitation, HA-oligo competition experiments confirmed that the UNIVmAb reactive 57 kDa antigen is a member of hyaladherin. Further Proteomic analysis showed that the antigen has homology with IGHG1 (Igγ-1 chain C region), IGg superfamily, and is associated with human serum albumin.

Typical Fluorescent Sensors Exploiting Molecularly Imprinted Hydrogels for Environmentally and Medicinally Important Analytes Detection

“…The images shown in Figure 4 A,B correspond to the fluorescence intensity analysis for Hela cells incubated with MIPG_GA or NIPG_GA, respectively, indicating that the MIPG_GA specifically bound towards the epitope of hyaluronic acid on Hela cells. Our fluorescent probe is more convenient for commercializing due to its excellent stability, though less sensitive with respect to the hyaluronic acid-binding protein (HABP, Figure S6 ) [ 35 , 36 , 37 ]. Moreover, compared with nano-sized MIP containing quantum dots [ 28 , 29 ], our MIPG_GA is easier to fabricate using a broad-spectrum cross-linker FAM-based monomer for signaling, showing the universal utility of this strategy.…”