Universal Primers for Detection of Common Bacterial Pathogens Causing Prosthetic Joint Infection

Šauer, Pavel; Gallo, Jiří; Kesselová, Michaela; Kolář, Milan; Koukalová, Dagmar

doi:10.5507/bp.2005.043

Cited by 24 publications

(19 citation statements)

References 23 publications

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“…The sequence of primers was used as follows: outer forward UNI_OL 5 0 -GTGTAGCGGTGAAATGCG-3 0 , outer reverse UNI_OR 5 0 -CGGGCGGTGTGTACAA-3 0 , inner forward UNI_IL 5 0 -GGTGGAG-CATGTGGTTTA-3 0 , inner reverse UNI_IR 5 0 -CCATTGTAG-CACGTGTGT-3 0 . Amplicons of expected sizes 709 bp and 287 bp were detected with ethidium bromide, 2% agarose gel after the first and the second run of nested-PCR [10].…”

Section: Pcrmentioning

confidence: 99%

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Paluch-Oleś¹,

Magryś²,

Kozioł-Montewka³

et al. 2011

International Journal of Pediatric Otorhinolaryngology

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Section: Pcrmentioning

confidence: 99%

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Paluch-Oleś¹,

Magryś²,

Kozioł-Montewka³

et al. 2011

International Journal of Pediatric Otorhinolaryngology

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“…Nowadays, PCR-based methods, in particular quantitative PCR, are used primarily to identify and quantify either pathogens or beneficial populations, including Bacillus sp., Campylobacter sp., Legionella sp., Pseudomonas sp., Salmonella sp., and Vibrio sp., etc., based on the 16S rRNA genes or their specific functional genes (Amri et al 2007;Anbazhagan et al 2010;Brolund et al 2010;dos Santos et al 2001;Fiume et al 2005;Goarant and Merien 2006;Le Dréan et al 2010;Maligoy et al 2008;Masco et al 2007;Mashsouf et al 2008;Nakano et al 2003;Postollec et al 2011;Sauer et al 2005;Smith and Osborn 2008;Sun et al 2009;Vanniasinkam et al 1999;Wehrle et al 2010). Development of multiple PCR to simultaneously detect common pathogens has also been recommended, such as the PCR methods developed to detect Enterobacteriaceae and clinically important bacteria (Cheng et al 2006;Lu et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

et al. 2012

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Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0× 10 3 CFU ml −1 was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml −1 was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.

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“…Previous use of antibiotics has been implicated as one of the main causes of this problem (Trampuz et al 2007), but other causes are also possible. To solve the problem, molecular biological techniques have been proposed in order to obtain faster and more accurate results than conventional culture (Tunney et al 1999, Sauer et al 2005, Dempsey et al 2007, Fihman et al 2007, Moojen et al 2007, Gallo et al 2008, Kobayashi et al 2008, Vandercam et al 2008, De Man et al 2009, Piper et al 2009, Achermann et al 2010, Riggio et al 2010, Marin et al 2012). Most of these reports were based on protocols that were developed in-house, which are difficult to integrate into clinical microbiology routines, even though they may give good results.…”

mentioning

confidence: 99%

PCR-hybridization after sonication improves diagnosis of implant-related infection

Esteban¹,

Alonso-Rodriguez²,

del-Prado³

et al. 2012

Acta Orthopaedica

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PurposeWe wanted to improve the diagnosis of implant-related infection using molecular biological techniques after sonication.MethodsWe studied 258 retrieved implant components (185 prosthetic implants and 73 osteosynthesis implants) from 126 patients. 47 patients had a clinical diagnosis of infection (108 components) and 79 patients did not (150 components). The fluids from sonication of retrieved implants were tested in culture and were also analyzed using a modified commercial PCR kit for detection of Gram-positive and Gram-negative bacteria (GenoType BC; Hain Lifescience) after extraction of the DNA.Results38 of 47 patients with a clinical diagnosis of infection were also diagnosed as being infected using culture and/or PCR (35 by culture alone). Also, 24 patients of the 79 cases with no clinical diagnosis of infection were identified microbiologically as being infected (4 by culture, 16 by PCR, and 4 by both culture and PCR). Comparing culture and PCR, positive culture results were obtained in 28 of the 79 patients and positive PCR results were obtained in 35. There were 21 discordant results in patients who were originally clinically diagnosed as being infected and 28 discordant results in patients who had no clinical diagnosis of infection.InterpretationFor prosthetic joint infections and relative to culture, molecular detection can increase (by one tenth) the number of patients diagnosed as having an infection. Positive results from patients who have no clinical diagnosis of infection must be interpreted carefully.

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Universal Primers for Detection of Common Bacterial Pathogens Causing Prosthetic Joint Infection

Cited by 24 publications

References 23 publications

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

PCR-hybridization after sonication improves diagnosis of implant-related infection

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