The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Paluch-Oleś, Jolanta; Magryś, Agnieszka; Kozioł-Montewka, Maria; Niedzielski, Artur; Niedźwiadek, Justyna; Niedzielska, G; Kotowski, Michał

doi:10.1016/j.ijporl.2010.10.025

Cited by 27 publications

(24 citation statements)

References 20 publications

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“…The entire ica operon was also observed in other investigations (de Silva et al, 2002;Koskela et al, 2009;Ninin et al, 2006), together with the icaR when it was investigated (Arciola et al, 2005;Cafiso et al, 2004). However, some studies have reported the detection of isolated ica genes (Diemond-Hernández et al, 2010;Cunha, 2010;Paluch-Oleś et al, 2011), sometimes in the absence of icaR gene (Esteban et al, 2010).…”

Section: Discussionsupporting

confidence: 58%

Biofilm production by clinical isolates of Staphylococcus epidermidis and its relationship with genotypic profile, presence of virulence-related genes and antibiotic resistance

Filho¹,

Silva²,

Saramago³

et al. 2015

Afr. J. Microbiol. Res.

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Staphylococcus epidermidis is considered as the main infectious agent associated with implanted medical devices. This study determined biofilm production and composition, pulsed-field gel electrophoresis (PFGE) profile, antimicrobial susceptibility and presence of virulence-related genes (ica operon, aap, bhp, embp, capB and IS256 transposase) in 49 clinical isolates of S. epidermidis. Twenty-five isolates (51%) were classified as biofilm producers in microtiter plate (MTP) assay. In Congo red assay (CRA) test, 14 (29%) showed positive reaction and three (6%) had indeterminate reaction, all were biofilmproducers in MTP assay. Fourteen isolates with positive reaction in CRA test had the chemical nature of biofilm determined as polysaccharide, had the ica operon (PIA-dependent producers) and the majority was strong biofilm producer. Eight biofilm producer isolates showed negative reaction in CRA test and the chemical nature of their biofilm was proteinaceous (PIA-independent producers). Antimicrobial resistance rates were generally higher in biofilm producers and resistance to beta-lactams ranged from 82-96%, while 61% of the isolates were multidrug resistant (≥ 10 drugs). Resistance to daptomycin, quinupristin/dalfopristin, rifampin and trimethoprim/sulfamethoxazole was observed only in PIAdependent isolates, while the resistance to gentamicin was present in all PIA-independent isolates and in just 53% of PIA-dependent of isolates. The most prevalent virulence-related genes were capB (80%) and embp (67%); the other genes were less frequent: ica operon (41%), aap (31%), IS256 transposase (22%) and bhp (10%). The presence of ica operon and IS256 transposase gene showed significant association with biofilm production and strong biofilm production. Moreover, these isolates presented significant higher resistance to levofloxacin, moxifloxacin, rifampin and trimethoprim/sulfamethoxazole. PFGE analysis showed 23 profiles, having the prevalent type 15 isolates. Of these, seven were PIAindependent biofilm producers and just one was PIA-dependent producer, unlike what was observed in other studies, where isolates of prevalent profiles were PIA-dependent biofilm producers.

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Section: Discussionsupporting

confidence: 58%

Biofilm production by clinical isolates of Staphylococcus epidermidis and its relationship with genotypic profile, presence of virulence-related genes and antibiotic resistance

Filho¹,

Silva²,

Saramago³

et al. 2015

Afr. J. Microbiol. Res.

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“…Interestingly, recent studies have shown that CNS is more prevalent in MEEF than are the three traditional pathogens. This may be because of variation in bacterial prevalence over time, antibiotic use, and/or vaccination against H. influenzae and S. pneumoniae [12,14]. …”

Section: Discussionmentioning

confidence: 99%

Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion

Lee

Kim

Lee

et al. 2013

Clin Exp Otorhinolaryngol

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ObjectivesToll-like receptor (TLR)-9 recognizes unmethylated cytidine-phosphate-guanosine (CpG) motifs in bacteria. Therefore, the expression of TLR-9 may differ according to the results of bacterial culture, and thus a change in proinflammatory cytokine induction can also be expected. The authors aimed to assess the differences and relationships between the expression of TLR-9, cytokines, and nitric oxide synthase (NOS) in otitis media with effusion (OME) based on bacterial culture results.MethodsSixty-eight patients with OME were divided into culture-positive and culture-negative groups based on middle ear culture results. mRNA expression of TLR-9, NOS, and cytokines was measured and analyzed.ResultsBacteria were detected in 38.2% of patients, and the distribution was as follows: coagulase negative Staphylococcus (10.3%), Staphylococcus aureus (8.8%), Streptococcus pneumonia (5.9%), and Bacillus spp. and Haemophilus influenza combined (2.9%). There were no significant differences in epidemiologic characteristics according to the culture results. Down-regulation of TLR-9 was observed in the culture-positive group (P=0.019). Cytokines including interleukin (IL)-12 (r=-0.582), tumor necrosis factor (TNF)-α (r=-0.569), interferon (IFN)-γ (r=-0.442), IL-6 (r=-0.395) and inducible NOS (r=-0.256) tended to decrease with the detection of bacteria.ConclusionThe expression of TLR-9 significantly decreased in OME with confirmed bacterial pathogens. IL-12, TNF-α, IFN-β, IL-6 expression tended to decrease with the detection of bacteria. The presence of bacterial pathogens in OME may be related to abnormalities in the innate immune system.

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“…Many features of otitis media support biofilm ear infection. This is formed by bacteria growing in slimeenclosed aggregates and provides an explanation for the lack of success of antibiotic therapy, the presence of negative bacterial cultures, and persistence of bacteria in the middleear space between acute episodes and local inflammatory responses to persistent bacteria [39]. The ability to form a biofilm by opportunistic coagulase-negative staphylococci strains emphasizes the pathogenic character of these strains in some cases of otitis media with effusion.…”

Section: Discussionmentioning

confidence: 99%

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Kosikowska

Chwiejczak

Niedzielski

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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short duration of illness and an acute beginning. OME is the clinical term for the presence of fluid in the middle ear behind the intact eardrum and is characterized by a nonpurulent effusion that may be either mucoid (thick) or serous (thin) [4]. It is an inflammation of the middle ear without signs or symptoms of acute ear infection, pending beyond its intact eardrum and without systemic or otoscopic symptoms. An OME is the catchall term if the tympanic effusion persists for more than 3 months. Near chronic ARTICLE INFO ABSTRACT Introduction. Haemophilus influenzae and Haemophilus parainfluenzae are known as human-restricted respiratory microbiota representatives. The aim of the present paper was to assay haemophili prevalence in middle ear effusion specimens in pediatric patients with otitis media with effusion (OME). Methods. A total of 86 ear effusion specimens (from the left and right ear independently) were collected from 43 pediatric patients with OME. For comparison, 58 nasopharyngeal specimens were taken from 58 pediatric patients prone to recurrent respiratory tract infections (RRTI). Isolation and identification of haemophili biotypes and antimicrobial susceptibility was accomplished by standard microbiological methods. The cell surface hydrophobicity (CSH) of isolates was assayed by the method of aggregation in ammonium sulfate (SAT). Results. Haemophili were isolated in 25.6% (11/43) of all OME patients: in 5/43 (11.6%) -H. influenzae (biotypes II, III), in 5/43 (11.6%) -H. parainfluenzae, in 1/43 (2.3%) -both species were found. Haemophili-positive nasopharyngeal specimen was found in 27/58 (46.6%) RRTI patients: in 19/58 (32.8%) -H. influenzae, in 8/58 (13.8%) -H. parainfluenzae. About 90% of all haemophili isolates were characterised by extreme to strong CSH. Antimicrobial resistance occurred mainly among H. parainfluenzae (80%) and to a much lower percentage among H. influenzae (33.3%) isolates. The obtained data suggest that both H. influenzae and H. parainfluenzae can be involved in pathology of OME in pediatric patients. The high cell surface hydrophobicity can affect on the haemophili prevalence and ear colonization, and induces predisposition to the presence of these bacteria as a biofilm that serves as a virulence factor with great importance for the survival of these opportunistic bacteria and their persistence in the ear environment.

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The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Cited by 27 publications

References 20 publications

Biofilm production by clinical isolates of Staphylococcus epidermidis and its relationship with genotypic profile, presence of virulence-related genes and antibiotic resistance

Biofilm production by clinical isolates of Staphylococcus epidermidis and its relationship with genotypic profile, presence of virulence-related genes and antibiotic resistance

Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

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