Unique genetic cassettes in a Thermoanaerobacterium contribute to simultaneous conversion of cellulose and monosugars into butanol

Abstract

We present a genome-level understanding of how cellulose is metabolized by Thermoanaerobacterium for biobutanol production.

Genomic and metabolic insights into solvent production by Thermoanaerobacterium thermosaccharolyticum GSU5

“…In recent years, analysis of the genome of several strains of T. thermosaccharolyticum revealed important differences in the biosynthesis of butanol and butyrate when compared to the ABE pathway known in the well characterized C. acetobutylicum (Jones and Woods, 1986). Previous reports indicated that the gene coding for the acetoacetate decarboxylase (adc) was absent in T. thermosaccharolyticum M5 (Jiang et al, 2018), and that both adc and ctfAB (that codes for the butyrate-acetoacetate CoA-transferase subunits A and B) were absent in T. thermosaccharolyticum TG57 (Li et al, 2018), indicating that these bacteria were unable to produce acetone. These genes were searched in the genomes of T. thermosaccharolyticum GSU5 and in all available Thermoanaerobacterium genomes using the genes from C. acetobutylicum as queries.…”

“…xylanolyticum, and T. butyriciformans (Onyenwoke and Wiegel, 2015). T. saccharolyticum and T. thermostercoris can produce ethanol (Shaw et al, 2008; Romano et al, 2010) while several T. thermosaccharolyticum isolates have been reported to produce hydrogen (Cao et al, 2009) and/or butanol (Li et al, 2018) from different biomass sources.…”

Modulation of organic compounds of spent fungi of a common fungus in biohumus when using an extracone biopreparation

“…Утилізувати відпрацьований субстрат гливи звичайної можна шляхом використання як кормової добавки до раціону птиці, свиней та ВРХ [1]. Відомий спосіб утилізації субстрату гливи для отримання кормопродукту і біогумусу за допомогою ентомологічної біотехнологічної переробки, де з метою утилізації використовують личинки мух Чорна львінка (Hermetia illucens, L.) [2]. Недоліком цього методу є тривалий період трансформації, висока температура для протікання цього процесу та неповна трансформація щодо ферментації за допомогою консорціуму ґрунтових мікроорганізмів.…”

Expansion segments in bacterial and archaeal 5S ribosomal RNAs

“…However, these conclusions were disproven after the whole genome sequences of several Th. thermosaccharolyticum strains became available in the 2010s (Jiang et al 2018, Li et al 2018, Hemme et al 2010. It is now known that this oversized 5S rRNA is encoded separately from the normal 5S rRNA, has a total length of 155 nucleotides, and contains a single 46-nucleotide insertion embedded deep within an otherwise typical 5S rRNA core sequence.…”