Unique diversity content of a thermophilic anaerobic microbial consortium that utilizes propionate in a synthetic medium

Sugihara, Takafumi; Shiratori, Hatsumi; Domoto, Rieko; Miyake, Taku; Yaegashi, Midori; Ayame, Shohei; Kataoka, Naoki; Miya, Akiko; Beppu, Teruhiko; Uéda, Kenji

doi:10.2323/jgam.53.363

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Despite the lower phylogenetic relationship between OTU_T H S and T. phaeum , the thermophilic growth condition was a shared capability of these species. Searches in the Blast database furthermore revealed that sequences with identical identity to OTU_T H S have been discovered in syntrophic propionate‐degrading communities (Sugihara et al ., ), indicating ability to degrade both propionate and acetate or involvement in acetate degradation in association with syntrophic propionate‐degrading bacteria. 16S rRNA gene sequences identical to OTU_T H S have previously also been found in thermophilic (53°C) dry anaerobic digestion of waste paper‐based medium and suggested to be involved in SAO (OTU 1‐1B‐29 in Tang et al ., ) The fhs gene sequencing indicated that the gene from OTU_T H S was not targeted by the current primers.…”

Section: Discussionmentioning

confidence: 99%

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm

Müller

Singh

et al. 2017

Microbial Biotechnology

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SummaryTo enrich syntrophic acetate‐oxidizing bacteria (SAOB), duplicate chemostats were inoculated with sludge from syntrophic acetate oxidation (SAO)‐dominated systems and continuously supplied with acetate (0.4 or 7.5 g l−1) at high‐ammonia levels. The chemostats were operated under mesophilic (37°C) or thermophilic (52°C) temperature for about six hydraulic retention times (HRT 28 days) and were sampled over time. Irrespective of temperature, a methane content of 64–69% and effluent acetate level of 0.4–1.0 g l−1 were recorded in chemostats fed high acetate. Low methane production in the low‐acetate chemostats indicated that the substrate supply was below the threshold for methanization of acetate via SAO. Novel representatives within the family Clostridiales and genus Syntrophaceticus (class Clostridia) were identified to represent putative SAOB candidates in mesophilic and thermophilic conditions respectively. Known SAOB persisted at low relative abundance in all chemostats. The hydrogenotrophic methanogens Methanoculleus bourgensis (mesophilic) and Methanothermobacter thermautotrophicus (thermophilic) dominated archaeal communities in the high‐acetate chemostats. In line with the restricted methane production in the low‐acetate chemostats, methanogens persisted at considerably lower abundance in these chemostats. These findings strongly indicate involvement in SAO and tolerance to high ammonia levels of the species identified here, and have implications for understanding community function in stressed anaerobic processes.

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Section: Discussionmentioning

confidence: 99%

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm

Müller

Singh

et al. 2017

Microbial Biotechnology

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“…Thermacetogenium ‐related sequences (e.g. GU984434) which were closely affiliated with the clone sequences of FM956805 (91% sequence similarity) retrieved from a hyperthermophilic anaerobic glucose‐degrading reactor (Tang et al ., 2008) and AB332113 (91%) retrieved from a thermal methanogenic bioreactor utilizing propionate (Sugihara et al ., 2007) demonstrated that these detected taxa play a ubiquitously important role in anaerobic digestion under thermal conditions.…”

Section: Discussionmentioning

confidence: 99%

Thermoanaerobacteriaceae oxidize acetate in methanogenic rice field soil at 50°C

Liu

Conrad²

2010

Environmental Microbiology

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“…The D3 OTU commonly detected from the three samples is closely related (100% identity) to uncultured Coprothermobacteria bacterium clone from one of the anaerobic digesters studied by Riviere et al The D5 OTU is close to Thermodesulfovibrio aggregan TGE-P1, a known thermophilic sulfate reducer isolated from methanogenic anaerobic sludge . The D6 OTU was close (99.5% identity) to an uncultured Bacillus bacterium clone obtained from a thermophilic anaerobic consortium using propionate . The D2, D4, D7, and D8 OTUs were not identifiable because of their identities lower than 97%.…”

Section: Resultsmentioning

confidence: 53%

“…32 The D6 OTU was close (99.5% identity) to an uncultured Bacillus bacterium clone obtained from a thermophilic anaerobic consortium using propionate. 33 The D2, D4, D7, and D8 OTUs were not identifiable because of their identities lower than 97%.…”

Section: ■ Resultsmentioning

confidence: 99%

Treatment of Alcohol Distillery Wastewater Using a Bacteroidetes-Dominant Thermophilic Microbial Fuel Cell

Lee²,

Rittmann

et al. 2012

Environ. Sci. Technol.

107

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Simultaneous electricity generation and distillery wastewater (DWW) treatment were accomplished using a thermophilic microbial fuel cell (MFC). The results suggest that thermophilic MFCs, which require less energy for cooling the DWW, can achieve high efficiency for electricity generation and also reduce sulfate along with oxidizing complex organic substrates. The generated current density (2.3 A/m(2)) and power density (up to 1.0 W/m(2)) were higher than previous wastewater-treating MFCs. The significance of the high Coulombic efficiency (CE; up to 89%) indicated that electrical current was the most significant electron sink in thermophilic MFCs. Bacterial diversity based on pyrosequencing of the 16S rRNA gene revealed that known Deferribacteres and Firmicutes members were not dominant in the thermophilic MFC fed with DWW; instead, uncharacterized Bacteroidetes thermophiles were up to 52% of the total reads in the anode biofilm. Despite the complexity of the DWW, one single bacterial sequence (OTU D1) close to an uncultured Bacteriodetes bacterium became predominant, up to almost 40% of total reads. The proliferation of the D1 species was concurrent with high electricity generation and high Coulombic efficiency.

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Unique diversity content of a thermophilic anaerobic microbial consortium that utilizes propionate in a synthetic medium

Cited by 6 publications

References 13 publications

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Thermoanaerobacteriaceae oxidize acetate in methanogenic rice field soil at 50°C

Treatment of Alcohol Distillery Wastewater Using a Bacteroidetes-Dominant Thermophilic Microbial Fuel Cell

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