Uniform-size Macroporous Polymer-based Stationary Phase for HPLC Prepared through Molecular Imprinting Technique

Hosoya, Ken; Yoshizako, Kimihiro; Tanaka, Nobuo; Kimata, Kazuhiro; Araki, Takeo; Haginaka, Jun

doi:10.1246/cl.1994.1437

Cited by 89 publications

(51 citation statements)

References 4 publications

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“…24 The SMIPs, which could be used as an HPLC stationary phase after simple washing, exhibited better flow characteristics in chromatography and higher load capacities compared with ordinary bulk polymers and monolithic polymers. 25 On the other hand, because polar solvents, especially water, can weaken the interaction between template molecules and functional monomers, most noncovalently imprinted polymers were prepared in less-polar solvents. From a practical point of view, however, much more research is required to extend the scope of the environment, the aqueous system for example, in which imprinting process can be performed.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Zhang

et al. 2006

ANAL. SCI.

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Ephedrine-imprinted polymeric microspheres have been prepared in an aqueous system by multi-step swelling and suspension polymerization, using methacrylic acid (MAA) as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. Scanning electron microscopy (SEM) was used as a means to identify the structure features of the obtained polymers. Further, we examined the recognition mechanism of the polymers and the influences of some chromatographic conditions, such as the mobile-phase composition, flow-rate, column temperature and sample amount on the retentivity and selectivity for (-)-ephedrine and (+)-ephedrine. The results reveal that stable macroporous polymer beads with good size monodispersity were obtained, the average size of which was 3 -5 μm. Baseline chiral separation of the template isomers was achieved on a short column (50 mm × 4.6 mm i.d.) when the prepared polymer beads were used as a stationary phase, while the non-imprinted polymers (NIPs) did not show such ability. The optimized chromatographic condition was as follows: acetonitrile-acetic acid (99.8/0.2, v/v) as the mobile phase; sample amount, 40 -80 μg; flow rate, 1.0 ml min -1 ; and column temperature, room temperature, respectively. It is assumed that two classes of binding sites exist in the porous polymers, one being hydrophilic binding sites, the other being hydrophobic binding sites.

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Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Zhang

et al. 2006

ANAL. SCI.

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“…Diante destas considerações, novas concepções de preparo dos MIP são relatadas na literatura com intuito de produzir partículas com tamanhos uniformes. Ao contrário do método de polimerização em "bulk", cuja síntese é realizada num sistema homogêneo, a maioria dos novos métodos de preparo contemplam reações em sistemas heterogêneos, incluindo a polimerização por suspensão 28 , por precipitação 48 e por expansão em multi-etapas 49 . Outros méto-dos de preparo dos MIP contemplam reações de polimerização dentro de poros de sólidos (sílica e resinas) 50,51 e reações de polimerização sobre superfície modificada de suporte sólido 52 .…”

Section: Métodos Recentes De Preparo Dos Mipunclassified

Polímeros biomiméticos em química analítica. Parte 1: preparo e aplicações de MIP ("Molecularly Imprinted Polymers") em técnicas de extração e separação

2005

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“…To overcome this problem, the MIPs, which give uniformly-sized and monodispersed particles, have been prepared using a multi-step swelling and polymerization method. 9 The MIPs prepared by this method have been applied for chiral separations of drugs, 10,11 and on-line extractions of drugs 12 and environmental pollutants 13 using aqueous mobile phases. This is because the use of aqueous mobile phases is desirable for the biomedical and environmental applications.…”

Section: Introductionmentioning

confidence: 99%

Retentivity and Enantioselectivity of Uniformly-sized Molecularly Imprinted Polymers for (S)-Nilvadipine in Aqueous and Non-Aqueous Mobile Phases

2005

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Uniformly-sized molecularly imprinted polymers (MIPs) for (S)-nilvadipine have been prepared by a multi-step swelling and polymerization method using methacrylic acid or 4-vinylpyridine (4-VPY) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linker, and toluene, chloroform, cyclohexanol or phenylacetonitrile as a porogen. The chiral recognition abilities of the MIPs for nilvadipine were evaluated using aqueous and non-aqueous mobile phases. Among the MIPs, the (S)-nilvadipine-imprinted 4-VPY-co-EDMA polymers prepared using toluene as a porogen showed the highest recognition ability for nilvadipine in both aqueous and non-aqueous mobile phases. In addition to molecular shape recognition, hydrogen-bonding interactions of the NH proton of nilvadipine with a pyridyl group of the (S)-nilvadipine-imprinted 4-VPY-co-EDMA polymers could play an important role in the retention and chiral recognition of nilvadipine in aqueous and non-aqueous mobile phases. Furthermore, the MIP for (S)-nilvadipine gave the highest molecular recognition ability when a porogenic solvent during polymerization was used as the mobile phase modifier.

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Uniform-size Macroporous Polymer-based Stationary Phase for HPLC Prepared through Molecular Imprinting Technique

Cited by 89 publications

References 4 publications

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Polímeros biomiméticos em química analítica. Parte 1: preparo e aplicações de MIP ("Molecularly Imprinted Polymers") em técnicas de extração e separação

Retentivity and Enantioselectivity of Uniformly-sized Molecularly Imprinted Polymers for (S)-Nilvadipine in Aqueous and Non-Aqueous Mobile Phases

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